Anti-CD90 / Thy1 antibody [7E1B11]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD90 / Thy1 antibody [7E1B11] (AB181469)

Immunohistochemical analysis of paraffin-embedded Human cerebellum tissue labeling CD90 / Thy1 with ab181469 at 1/200 dilution with DAB staining.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CD90 / Thy1 antibody [7E1B11] (AB181469)

Immunofluorescent analysis of HeLa cells labeling CD90 / Thy1 with ab181469 at 1/200 dilution (green). Blue : DRAQ5 fluorescent DNA dye. Red : Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD90 / Thy1 antibody [7E1B11] (AB181469)

Immunohistochemical analysis of paraffin-embedded Human endometrial cancer tissue labeling CD90 / Thy1 with ab181469 at 1/200 dilution with DAB staining.

• WB

Lab

Western blot - Anti-CD90 / Thy1 antibody [7E1B11] (AB181469)

Anti-THY1 antibody [7E1B11] (ab181469) staining at 1/1000 dilution, shown in green; Rabbit anti-alpha Tubulin antibody [EP1332Y] (ab52866) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab181469 was shown to bind specifically to THY1. A band was observed at 25-37 kDa in wild-type U-2 OS cell lysates with no signal observed at this size in THY1 knockout cell line ab262490 (knockout cell lysate ab263925). To generate this image, wild-type and THY1 knockout U-2 OS cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L 800CW and Goat anti-Rabbit IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-CD90 / Thy1 antibody [7E1B11] (ab181469) at 1/1000 dilution

Lanes 1 - 5:

Western blot at 20 µg

Lane 2:

Western blot - Human THY1 (CD90) knockout U-2 OS cell lysate (<a href='/en-us/products/cell-lysates/human-thy1-cd90-knockout-u-2-os-cell-lysate-ab263925'>ab263925</a>)

Lane 2:

Western blot - Human THY1 (CD90) knockout U-2 OS cell line (<a href='/en-us/products/cell-lines/human-thy1-cd90-knockout-u-2-os-cell-line-ab262490'>ab262490</a>)

Secondary

All lanes:

Goat anti-Mouse IgG H&L 800CW and Goat anti-Rabbit IgG H&L 680RD at 1/20000 dilution

Predicted band size: 17 kDa

false

• WB

Lab

Western blot - Anti-CD90 / Thy1 antibody [7E1B11] (AB181469)

False colour image of Western blot : Anti-CD90 / Thy1 antibody [7E1B11] staining at 1/2000 dilution, shown in green; Rabbit anti-alpha Tubulin antibody [EP1332Y] (ab52866) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab181469 was shown to bind specifically to CD90 / Thy1. A band was observed at 35-50 kDa in wild-type U-2 OS cell lysates with no signal observed at this size in Thy1 knockout cell line ab262490 (knockout cell lysate ab263925). To generate this image, wild-type and Thy1 knockout U-2 OS cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L (IRDye^® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye^® 680RD) preabsorbed (ab216777) at 1/20000 dilution.

All lanes:

Western blot - Anti-CD90 / Thy1 antibody [7E1B11] (ab181469) at 1/2000 dilution

Lane 1:

Wild-type U-2 OS cell lysate at 20 µg

Lane 2:

THY1 knockout U-2 OS cell lysate at 20 µg

Lane 2:

Western blot - Human THY1 (CD90) knockout U-2 OS cell line (<a href='/en-us/products/cell-lines/human-thy1-cd90-knockout-u-2-os-cell-line-ab262490'>ab262490</a>)

Lane 3:

HepG2 cell lysate at 20 µg

Lane 4:

PC-12 cell lysate at 20 µg

Lane 5:

Human Brain cell lysate at 20 µg

Predicted band size: 17 kDa

Observed band size: 30-50 kDa,35-50 kDa

false

• WB

Supplier Data

Western blot - Anti-CD90 / Thy1 antibody [7E1B11] (AB181469)

All lanes:

Western blot - Anti-CD90 / Thy1 antibody [7E1B11] (ab181469) at 1/500 dilution

Lane 1:

Non-transfected HEK293 cell lysate

Lane 2:

CD90 / Thy1 (aa 17-132)-hIgGFc transfected HEK293 cell lysate

Predicted band size: 17 kDa

false

• WB

Supplier Data

Western blot - Anti-CD90 / Thy1 antibody [7E1B11] (AB181469)

Expected MWt is 38.5 kDa.

All lanes:

Western blot - Anti-CD90 / Thy1 antibody [7E1B11] (ab181469) at 1/500 dilution

All lanes:

CD90 / Thy1 recombinant protein

Predicted band size: 17 kDa

false

• WB

Supplier Data

Western blot - Anti-CD90 / Thy1 antibody [7E1B11] (AB181469)

All lanes:

Western blot - Anti-CD90 / Thy1 antibody [7E1B11] (ab181469) at 1/500 dilution

Lane 1:

T47D cell lysate

Lane 2:

HepG2 cell lysate

Lane 3:

PC-12 cell lysate

Predicted band size: 17 kDa

false