AB23894

Anti-CD90 / Thy1 antibody [AF-9]

Name: Anti-CD90 / Thy1 antibody [AF-9] (ab23894) | Abcam
Brand: Abcam Limited
SKU: AB23894
Price: 252 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(33 Publications)

Mouse Monoclonal CD90 / Thy1 antibody. Suitable for Flow Cyt and reacts with Human samples. Cited in 33 publications. Immunogen corresponding to Cell preparation containing THY1 protein.

View Alternative Names

CD90, Thy-1 membrane glycoprotein, CDw90, Thy-1 antigen, THY1

1 Images

Flow Cytometry - Anti-CD90 / Thy1 antibody [AF-9] (AB23894)

Flow Cyt

Unknown

Flow Cytometry - Anti-CD90 / Thy1 antibody [AF-9] (AB23894)

Overlay histogram showing Jurkat cells stained with ab23894 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab23894, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

AF-9

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing THY1 protein. The exact immunogen used to generate this antibody is proprietary information.

P04216

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }

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Why is this recommended?

We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 7.3 Preservative: 0.05% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD90 also known as Thy1 is a glycoprotein with a molecular mass of approximately 25-37 kDa. It is highly conserved across species and found on the surface of various cell types including fibroblasts neurons and endothelial cells. CD90 is expressed in these cells in tissues such as the brain thymus and lungs. Researchers use anti-CD90 antibodies for detection often in combination with CD90 FITC in immunofluorescence assays. CD90 serves as a useful cell marker particularly in identifying mesenchymal stem cells (MSCs).

Biological function summary

CD90 plays a role in cell-cell and cell-matrix interactions contributing to cellular adhesion migration and signal transduction. It functions as part of a complex involving integrins which mediate its interaction with the extracellular matrix. This protein also influences T-cell activation reflecting its importance in immune response regulation. Its presence aids in modulating response to injury and promoting tissue repair due to its association with stem and progenitor cells.

Pathways

CD90 engagement affects focal adhesion and MAPK signaling pathways. It interacts with integrins like αvβ3 and α5β1 influencing cell movement and proliferation processes. CD90 involvement in these pathways allows it to regulate responses important for cell survival and communication within various tissues. These interactions highlight the protein's role in maintaining cellular homeostasis and regulating physiological processes.

CD90 has associations with fibrosis and cancer. In fibrotic conditions CD90 expression affects fibroblast activity influencing tissue scarring in organs such as the liver and lungs. In cancer CD90 modifies tumor progression and metastasis by impacting cell adhesion and migration with connections to proteins like TGF-beta which further drive these processes. Understanding CD90 in disease contexts facilitates the development of therapeutic strategies aimed at modulating its activity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.

See full target information THY1

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Publications (33)

Recent publications for all applications. Explore the full list and refine your search

Biomolecules & biomedicine : PubMed38059910

2023

Mechanism of adipose tissue-derived stromal cell-extracellular vesicles in treating oral submucous fibrosis by blocking the TGF-β1/Smad3 pathway via the miR-760-3p/IGF1R axis

Applications

Unspecified application

Species

Unspecified reactive species

Fengcong Wang,Li Jiang,Ping Liu,Yanjun Jiang

Stem cell research & therapy 14:344 PubMed38031182

2023

The effect of TLR3 priming conditions on MSC immunosuppressive properties.

Applications

Unspecified application

Species

Unspecified reactive species

Tatiana Tolstova,Ekaterina Dotsenko,Peter Kozhin,Svetlana Novikova,Victor Zgoda,Alexander Rusanov,Nataliya Luzgina

Bioactive materials 25:256-272 PubMed36825224

2023

"Slow walk" mimetic tensile loading maintains human meniscus tissue resident progenitor cells homeostasis in photocrosslinked gelatin hydrogel.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Sun,Yau Tsz Chan,Ki Wai Kevin Ho,Li Zhang,Liming Bian,Rocky S Tuan,Yangzi Jiang

Frontiers in oncology 12:1077024 PubMed36605441

2022

Stem-cell therapy gastroscopy improves the outcome of esophageal anastomotic leakage.

Applications

Unspecified application

Species

Unspecified reactive species

Yannan Hu,Heng Chu,Xiang Xue,Yan Yan,Wenbang Chen,Xilong Lang,Hao Zhang

Acta haematologica 146:505-517 PubMed36327876

2022

Extracellular Vesicles Derived from Human Bone Marrow Stem Cells Inhibit Acute Lymphoblastic Leukemia Cell Growth by Inhibiting MAPK Pathway via the miR-29b-3p/GDF15 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Li Zhou,Zhe Shan,Jiangsha Fan

Cellular & molecular biology letters 27:86 PubMed36209059

2022

Anti-aging effect of β-carotene through regulating the KAT7-P15 signaling axis, inflammation and oxidative stress process.

Applications

Unspecified application

Species

Unspecified reactive species

Wei V Zheng,Wang Xu,Yaqin Li,Jie Qin,Tao Zhou,Dezhi Li,Yanwei Xu,Xianyi Cheng,Yu Xiong,Zaizhong Chen

Bioengineering (Basel, Switzerland) 9: PubMed36134958

2022

Optimized Decellularization Protocol for Large Peripheral Nerve Segments: Towards Personalized Nerve Bioengineering.

Applications

Unspecified application

Species

Unspecified reactive species

Alois Hopf,Lina Al-Bayati,Dirk J Schaefer,Daniel F Kalbermatten,Raphael Guzman,Srinivas Madduri

Analytical cellular pathology (Amsterdam) 2022:9302496 PubMed35127344

2022

Extracellular Vesicle-Encapsulated MicroRNA-375 from Bone Marrow-Derived Mesenchymal Stem Cells Inhibits Hepatocellular Carcinoma Progression through Regulating HOXB3-Mediated Wnt/-Catenin Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zhaoxia Yu,Ju Liu,Qiqi Fan,Jun Yu,Xiaoting Ren,Xiaobin Wang

Heliyon 7:e07996 PubMed34589625

2021

Mesenchymal stem cells act as stimulators of neurogenesis and synaptic function in a rat model of Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Maryam Doshmanziari,Sadegh Shirian,Mohammad-Reza Kouchakian,Seyedeh Farzaneh Moniri,Saranaz Jangnoo,Niloofar Mohammadi,Fariba Zafari

Experimental and therapeutic medicine 22:1091 PubMed34504545

2021

Osteogenic ability using porous hydroxyapatite scaffold-based delivery of human placenta-derived mesenchymal stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaohua Ren,Qingwei Wang,Chunhui Liu,Qian Zhao,Jiajun Zheng,Kun Tian,Huijuan Xu,Yandong Mu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

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