Anti-CD90 / Thy1 antibody [EPR28145-53] KO tested (ab307736)

Knockout Tested Rabbit Recombinant Monoclonal CD90 / Thy1 antibody. Suitable for WB, ICC/IF, Flow Cyt, IP and reacts with Human, Mouse, Rat samples. Cited in 2 publications.

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Images

Immunoprecipitation - Anti-CD90 / Thy1 antibody [EPR28145-53] (AB307736), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	WB	ICC/IF	Flow Cyt	IP	IHC-P
Human	Tested	Tested	Tested	Tested	Not recommended
Mouse	Tested	Tested	Tested	Expected	Not recommended
Rat	Tested	Tested	Tested	Expected	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/1000 - 1/5000	Notes Observed molecular weight may vary depending on the glycosylation level of the target.
Species Mouse	Dilution info 1/1000 - 1/5000	Notes Observed molecular weight may vary depending on the glycosylation level of the target.
Species Rat	Dilution info 1/1000 - 1/5000	Notes Observed molecular weight may vary depending on the glycosylation level of the target.

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/250 - 1/1000	Notes -
Species Mouse	Dilution info 1/250 - 1/1000	Notes -
Species Rat	Dilution info 1/250 - 1/1000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/500	Notes -
Species Mouse	Dilution info 1/500	Notes -
Species Rat	Dilution info 1/500	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/30	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Target data

See full target information THY1

Function

May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.

Alternative names

CD90, Thy-1 membrane glycoprotein, CDw90, Thy-1 antigen, THY1

Recommended products

Knockout Tested Rabbit Recombinant Monoclonal CD90 / Thy1 antibody. Suitable for WB, ICC/IF, Flow Cyt, IP and reacts with Human, Mouse, Rat samples. Cited in 2 publications.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR28145-53
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CD90 also known as Thy1 is a glycoprotein with a molecular mass of approximately 25-37 kDa. It is highly conserved across species and found on the surface of various cell types including fibroblasts neurons and endothelial cells. CD90 is expressed in these cells in tissues such as the brain thymus and lungs. Researchers use anti-CD90 antibodies for detection often in combination with CD90 FITC in immunofluorescence assays. CD90 serves as a useful cell marker particularly in identifying mesenchymal stem cells (MSCs).

Biological function summary

CD90 plays a role in cell-cell and cell-matrix interactions contributing to cellular adhesion migration and signal transduction. It functions as part of a complex involving integrins which mediate its interaction with the extracellular matrix. This protein also influences T-cell activation reflecting its importance in immune response regulation. Its presence aids in modulating response to injury and promoting tissue repair due to its association with stem and progenitor cells.

Pathways

CD90 engagement affects focal adhesion and MAPK signaling pathways. It interacts with integrins like αvβ3 and α5β1 influencing cell movement and proliferation processes. CD90 involvement in these pathways allows it to regulate responses important for cell survival and communication within various tissues. These interactions highlight the protein's role in maintaining cellular homeostasis and regulating physiological processes.

Associated diseases and disorders

CD90 has associations with fibrosis and cancer. In fibrotic conditions CD90 expression affects fibroblast activity influencing tissue scarring in organs such as the liver and lungs. In cancer CD90 modifies tumor progression and metastasis by impacting cell adhesion and migration with connections to proteins like TGF-beta which further drive these processes. Understanding CD90 in disease contexts facilitates the development of therapeutic strategies aimed at modulating its activity.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

Immunoprecipitation - Anti-CD90 / Thy1 antibody [EPR28145-53] (ab307736)
CD90 / Thy1 was immunoprecipitated from 0.35 mg HuT-78 (human sezary syndrome cutaneous t lymphocyte) whole cell lysate 10 µg with ab307736 at 1/30 dilution (2 µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab307736 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: HuT-78 whole cell lysate 10 µg
Lane 2: ab307736 IP in HuT-78 whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab307736 in HuT-78 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 15 seconds.
The molecular weight observed is consistent with what has been described in the literature (PMID: 16770003) and due to the high glycosylation of the protein.
Immunoprecipitation - Anti-CD90 / Thy1 antibody [EPR28145-53] (ab307736)
CD90 / Thy1 was immunoprecipitated from 0.35 mg U-2 OS (human bone osteosarcoma epithelial cell) whole cell lysate 10 µg with ab307736 at 1/30 dilution (2 µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab307736 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: U-2 OS whole cell lysate 10 µg
Lane 2: ab307736 IP in U-2 OS whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab307736 in HU-2 OS whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 15 seconds.
The molecular weight observed is consistent with what has been described in the literature (PMID: 16770003) and due to the high glycosylation of the protein.
Western blot - Anti-CD90 / Thy1 antibody [EPR28145-53] (ab307736)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID: 16770003) and due to the highglycosylation of the protein.

Exposure time: 48 seconds.
All lanes: Western blot - Anti-CD90 / Thy1 antibody [EPR28145-53] (ab307736) at 1/1000 dilution
Lane 1: Human spleen tissue lysate at 20 µg
Lane 2: HuT-78 (human sezary syndrome cutaneous t lymphocyte) whole cell lysate at 20 µg
Lane 3: Mouse brain tissue lysate at 20 µg
Lane 4: Rat brain tissue lysate at 20 µg
Lane 5: PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 25 kDa, 37 kDa
Exposure time: 48s
Western blot - Anti-CD90 / Thy1 antibody [EPR28145-53] (ab307736)
Western blot: Anti-THY1 antibody [EPR28145-53] (ab307736) staining at 1/5000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab307736 was shown to bind specifically to THY1. A band was observed at 25-37 kDa in wild-type U-2 OS cell lysates with no signal observed at this size in THY1 knockout cell line Human THY1 (CD90) knockout U-2 OS cell line ab262490 (knockout cell lysate Human THY1 (CD90) knockout U-2 OS cell lysate ab263925). To generate this image, wild-type and THY1 knockout U-2 OS cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
All lanes: Western blot - Anti-CD90 / Thy1 antibody [EPR28145-53] (ab307736) at 1/5000 dilution
Lane 1: Wild-type U-2 OS cell lysate at 30 µg
Lane 2: Western blot - Human THY1 (CD90) knockout U-2 OS cell lysate (Human THY1 (CD90) knockout U-2 OS cell lysate ab263925)
Lane 2: Western blot - Human THY1 (CD90) knockout U-2 OS cell line (Human THY1 (CD90) knockout U-2 OS cell line ab262490)
Lane 2: THY1 knockout U-2 OS cell lysate at 30 µg
Lane 3: Human brain cell lysate at 2 µg
Lane 4: Human kidney cell lysate at 20 µg
Lane 5: K562 cell lysate at 30 µg
Secondary
All lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 17 kDa
Flow Cytometry (Intracellular) - Anti-CD90 / Thy1 antibody [EPR28145-53] (ab307736)
Flow cytometric analysis of PC-12 (rat adrenal gland pheochromocytoma) cells labeling CD90 / Thy1 with ab307736 at 1/500 dilution (0.1µg) (Red) compared with a Rabbit monoclonal IgG isotype control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.
Flow Cytometry (Intracellular) - Anti-CD90 / Thy1 antibody [EPR28145-53] (ab307736)
Flow cytometric analysis of Rabbit monoclonal IgG isotype control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Left panel) compared to mouse peripheral blood mononuclear cell (PBMC) (Right panel) labeling CD90 / Thy1 with ab307736 at 1/500 dilution (0.1 µg). Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody.
Cells were stained with rabbit IgG or ab307736 then stained with anti-CD3 conjugated to Alexa Fluor® 647.
Gated on viable cells.
Flow Cytometry (Intracellular) - Anti-CD90 / Thy1 antibody [EPR28145-53] (ab307736)
Flow cytometric analysis of K-562 (human chronic myelogenous leukemia lymphoblast) (Left panel) compared to U-2 OS (human bone osteosarcoma epithelial cell) (Right panel) labeling CD90 / Thy1 with ab307736 at 1/500 dilution (0.1 µg) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody.
Negative control: K-562.
Gated on viable cells.
Immunocytochemistry/ Immunofluorescence - Anti-CD90 / Thy1 antibody [EPR28145-53] (ab307736)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized PC-12 (rat adrenal gland pheochromocytoma small irregularly shaped cells) cells labeling CD90 / Thy1 with ab307736 at 1/250 dilution (1.8 µg/ml) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2 µg/ml) (Green).
Confocal image showing membranous and cytoplasmic staining in PC-12 cell line.
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 µg/ml) (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/ml).
Immunocytochemistry/ Immunofluorescence - Anti-CD90 / Thy1 antibody [EPR28145-53] (ab307736)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized EL4 (mouse lymphoma T lymphocyte) cells labeling CD90 / Thy1 with ab307736 at 1/250 dilution (1.8 µg/ml) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2 µg/ml) (Green).
Confocal image showing membranous and cytoplasmic staining in EL4 cell line.
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 µg/ml) (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/ml).
Immunocytochemistry/ Immunofluorescence - Anti-CD90 / Thy1 antibody [EPR28145-53] (ab307736)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell) cells labeling CD90 / Thy1 with ab307736 at 1/250 dilution (1.8 µg/ml) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2 µg/ml) (Green).
Confocal image showing membranous and cytoplasmic staining in U-2 OS cell line, no staining was observed in K-562 cell line.
Negative control: K-562 (PMID: 7683034).
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 µg/ml) (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/ml).