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Knockout Tested Rabbit Monoclonal CD90 / Thy1 antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 4 publications.

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Images

Western blot - Anti-CD90 / Thy1 antibody [EPR3133] - Low endotoxin, Azide free (AB216449), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-CD90 / Thy1 antibody [EPR3133] - Low endotoxin, Azide free (AB216449), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD90 / Thy1 antibody [EPR3133] - Low endotoxin, Azide free (AB216449), expandable thumbnail
  • Western blot - Anti-CD90 / Thy1 antibody [EPR3133] - Low endotoxin, Azide free (AB216449), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPFlow CytWBICC/IF
Human
Tested
Not recommended
Not recommended
Tested
Tested

Tested
Tested

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

Observed molecular weight may vary depending on the glycosylation level of the target.

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Associated Products

Select an associated product type

15 products for Alternative Product

6 products for Alternative Version

Target data

Function

May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.

Alternative names

Recommended products

Knockout Tested Rabbit Monoclonal CD90 / Thy1 antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 4 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR3133

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab216449 is the carrier-free version of Anti-CD90 / Thy1 antibody [EPR3133] ab133350.

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Our Low endotoxin, azide-free formats have low endotoxin level (1 EU/mg, determined by the TAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CD90 also known as Thy1 is a glycoprotein with a molecular mass of approximately 25-37 kDa. It is highly conserved across species and found on the surface of various cell types including fibroblasts neurons and endothelial cells. CD90 is expressed in these cells in tissues such as the brain thymus and lungs. Researchers use anti-CD90 antibodies for detection often in combination with CD90 FITC in immunofluorescence assays. CD90 serves as a useful cell marker particularly in identifying mesenchymal stem cells (MSCs).

Biological function summary

CD90 plays a role in cell-cell and cell-matrix interactions contributing to cellular adhesion migration and signal transduction. It functions as part of a complex involving integrins which mediate its interaction with the extracellular matrix. This protein also influences T-cell activation reflecting its importance in immune response regulation. Its presence aids in modulating response to injury and promoting tissue repair due to its association with stem and progenitor cells.

Pathways

CD90 engagement affects focal adhesion and MAPK signaling pathways. It interacts with integrins like αvβ3 and α5β1 influencing cell movement and proliferation processes. CD90 involvement in these pathways allows it to regulate responses important for cell survival and communication within various tissues. These interactions highlight the protein's role in maintaining cellular homeostasis and regulating physiological processes.

Associated diseases and disorders

CD90 has associations with fibrosis and cancer. In fibrotic conditions CD90 expression affects fibroblast activity influencing tissue scarring in organs such as the liver and lungs. In cancer CD90 modifies tumor progression and metastasis by impacting cell adhesion and migration with connections to proteins like TGF-beta which further drive these processes. Understanding CD90 in disease contexts facilitates the development of therapeutic strategies aimed at modulating its activity.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-CD90 / Thy1 antibody [EPR3133] - Low endotoxin, Azide free (ab216449), expandable thumbnail

    Western blot - Anti-CD90 / Thy1 antibody [EPR3133] - Low endotoxin, Azide free (ab216449)

    This data was developed using Anti-CD90 / Thy1 antibody [EPR3133] ab133350, the same antibody clone in a different buffer formulation.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 24116172 and PMID: 30177788).

    Blocking Buffer and concentration: 5% NFDM/TBST

    All lanes: Western blot - Anti-CD90 / Thy1 antibody [EPR3133] (Anti-CD90 / Thy1 antibody [EPR3133] ab133350) at 1/1000 dilution

    Lane 1: Human glioma lysate at 15 µg

    Lane 2: Human brain lysate at 15 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 17 kDa

    Observed band size: 25-35 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-CD90 / Thy1 antibody [EPR3133] - Low endotoxin, Azide free (ab216449), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CD90 / Thy1 antibody [EPR3133] - Low endotoxin, Azide free (ab216449)

    This data was developed using Anti-CD90 / Thy1 antibody [EPR3133] ab133350, the same antibody clone in a different buffer formulation.
    Immunocytochemistry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling CD90 / Thy1 with Purified ab216449 at 1:50 dilution (2.44 ?g/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD90 / Thy1 antibody [EPR3133] - Low endotoxin, Azide free (ab216449), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD90 / Thy1 antibody [EPR3133] - Low endotoxin, Azide free (ab216449)

    This data was developed using Anti-CD90 / Thy1 antibody [EPR3133] ab133350, the same antibody clone in a different buffer formulation.
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue sections labeling CD90 / Thy1 with Purified ab216449 at 1:4000 dilution (0.03 μg/ml). Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Western blot - Anti-CD90 / Thy1 antibody [EPR3133] - Low endotoxin, Azide free (ab216449), expandable thumbnail

    Western blot - Anti-CD90 / Thy1 antibody [EPR3133] - Low endotoxin, Azide free (ab216449)

    This data was developed using Anti-CD90 / Thy1 antibody [EPR3133] ab133350, the same antibody clone in a different buffer formulation.

    Anti-THY1 antibody [EPR3133] (Anti-CD90 / Thy1 antibody [EPR3133] ab133350) staining at 1/2000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, Anti-CD90 / Thy1 antibody [EPR3133] ab133350 was shown to bind specifically to THY1. A band was observed at 25-37 kDa in wild-type U-2 OS cell lysates with no signal observed at this size in THY1 knockout cell line Human THY1 (CD90) knockout U-2 OS cell line ab262490 (knockout cell lysate Human THY1 (CD90) knockout U-2 OS cell lysate ab263925). To generate this image, wild-type and THY1 knockout U-2 OS cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

    All lanes: Western blot - Anti-CD90 / Thy1 antibody [EPR3133] (Anti-CD90 / Thy1 antibody [EPR3133] ab133350) at 1/2000 dilution

    All lanes: Western blot at 20 µg

    Secondary

    All lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

    Performed under reducing conditions.

    Predicted band size: 17 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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