AB222781

Anti-CD90 / Thy1 antibody [MRC OX-7] - BSA and Azide free

Name: Anti-CD90 / Thy1 antibody [MRC OX-7] - BSA and Azide free (ab222781) | Abcam
Brand: Abcam Limited
SKU: AB222781
Price: 905 USD
Availability: InStock

Be the first to review this product! Submit a review

(4 Publications)

Mouse Monoclonal CD90 / Thy1 antibody. Carrier free. Suitable for ICC, Flow Cyt (Intra), WB and reacts with Rat samples. Cited in 4 publications.

View Alternative Names

CD90, Thy-1, Thy1, Thy-1 membrane glycoprotein, Thy-1 antigen

2 Images

Flow Cytometry - Anti-CD90 / Thy1 antibody [MRC OX-7] - BSA and Azide free (AB222781)

Flow Cyt

Unknown

Flow Cytometry - Anti-CD90 / Thy1 antibody [MRC OX-7] - BSA and Azide free (AB222781)

Flow cytometry staining of Lewis rat splenocytes with ab222781 (right) or mouse IgG1κ (ab170190) isotype (left). Cells were incubated for 30 min on ice in 1x PBS containing 10 % rat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab222781) or mouse IgG1κ (ab170190) isotype (1x10⁶ in 100 μL at 0.2 μg/ml) for 30 min on ice.

The secondary antibody Goat anti-mouse IgG H&L (Alexa Fluor ^® 488, pre-adsorbed) (ab150117) was used at dilution for 30 min on ice.

The cells were simultaneously stained with CD4.

Acquisition of >30000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on live CD3 positive T cells.

Flow Cytometry (Intracellular) - Anti-CD90 / Thy1 antibody [MRC OX-7] - BSA and Azide free (AB222781)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-CD90 / Thy1 antibody [MRC OX-7] - BSA and Azide free (AB222781)

The flow cytometry data shown was generated using the same antibody clone in a different buffer formulation (ab225).

Overlay histogram showing PC12 cells stained with ab225 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab225, 0.1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight^® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [B11/6](ab91353, 1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive result in 80% methanol (5 min) fixed PC12 cells used under the same conditions.

Unconjugated

Anti-CD90 / Thy1 antibody [MRC OX-7] - Hematopoietic Stem Cell Marker
519 FITC

FITC Anti-CD90 / Thy1 antibody [MRC OX-7]
HRP

HRP Anti-CD90 / Thy1 antibody [MRC OX-7]
578 PE

PE Anti-CD90 / Thy1 antibody [MRC OX-7]
675 PerCP

PerCP Anti-CD90 / Thy1 antibody [MRC OX-7]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MRC OX-7

Isotype

IgG1

Light chain type

kappa

Carrier free

Yes

Reacts with

Rat

Applications

WB, ICC, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "Observed molecular weight may vary depending on the glycosylation level of the target." }, "Guinea pig": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

Product details

The affinity of the Fab' of MRC OX-7 for rat Thy-1 is 3 x 10⁹m^-1 and for mouse Thy-1.1 is 3 x 10⁸m^-1.

ab222781 is the carrier-free version of ab225.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD90 also known as Thy1 is a glycoprotein with a molecular mass of approximately 25-37 kDa. It is highly conserved across species and found on the surface of various cell types including fibroblasts neurons and endothelial cells. CD90 is expressed in these cells in tissues such as the brain thymus and lungs. Researchers use anti-CD90 antibodies for detection often in combination with CD90 FITC in immunofluorescence assays. CD90 serves as a useful cell marker particularly in identifying mesenchymal stem cells (MSCs).

Biological function summary

CD90 plays a role in cell-cell and cell-matrix interactions contributing to cellular adhesion migration and signal transduction. It functions as part of a complex involving integrins which mediate its interaction with the extracellular matrix. This protein also influences T-cell activation reflecting its importance in immune response regulation. Its presence aids in modulating response to injury and promoting tissue repair due to its association with stem and progenitor cells.

Pathways

CD90 engagement affects focal adhesion and MAPK signaling pathways. It interacts with integrins like αvβ3 and α5β1 influencing cell movement and proliferation processes. CD90 involvement in these pathways allows it to regulate responses important for cell survival and communication within various tissues. These interactions highlight the protein's role in maintaining cellular homeostasis and regulating physiological processes.

CD90 has associations with fibrosis and cancer. In fibrotic conditions CD90 expression affects fibroblast activity influencing tissue scarring in organs such as the liver and lungs. In cancer CD90 modifies tumor progression and metastasis by impacting cell adhesion and migration with connections to proteins like TGF-beta which further drive these processes. Understanding CD90 in disease contexts facilitates the development of therapeutic strategies aimed at modulating its activity.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.

See full target information Thy1

style

left-column

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cellular & molecular biology letters 30:8 PubMed39833662

2025

Feedback loop centered on MAF1 reduces blood-brain barrier damage in sepsis-associated encephalopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Xuebiao Wei,Wenqiang Jiang,Zhonghua Wang,Yichen Li,Yuanwen Jing,Yongli Han,Linqiang Huang,Shenglong Chen

Progress in biomaterials 11:385-396 PubMed36271317

2022

Encapsulation of rat bone marrow-derived mesenchymal stem cells (rBMMSCs) in collagen type I containing platelet-rich plasma for osteoarthritis treatment in rat model.

Applications

Unspecified application

Species

Unspecified reactive species

Md Shahidul Islam,Somayeh Ebrahimi-Barough,Mamun Al Mahtab,Sadegh Shirian,Hamid Reza Aghayan,Babak Arjmand,Amir Allahverdi,Faezeh Esmaeili Ranjbar,Amin Bigham Sadeg,Jafar Ai

Molecular medicine reports 24: PubMed34414446

2021

Porcine pancreas mesenchymal cell characterization and functional differentiation into insulin‑producing cells .

Applications

Unspecified application

Species

Unspecified reactive species

Shang Zhang,Qi Wang,Hongbing Ji,Huidi Lu,Qin Yang,Jiahui Yin,Weijun Guan

Clinical cancer research : an official journal of 17:5392-401 PubMed21705450

2011

Induction of HLA-DP4-restricted anti-survivin Th1 and Th2 responses using an artificial antigen-presenting cell.

Applications

Unspecified application

Species

Unspecified reactive species

Makito Tanaka,Marcus O Butler,Sascha Ansén,Osamu Imataki,Alla Berezovskaya,Lee M Nadler,Naoto Hirano

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD90 / Thy1 antibody [MRC OX-7] - BSA and Azide free

Flow Cytometry - Anti-CD90 / Thy1 antibody [MRC OX-7] - BSA and Azide free (AB222781)

Flow Cytometry (Intracellular) - Anti-CD90 / Thy1 antibody [MRC OX-7] - BSA and Azide free (AB222781)

Related conjugates and formulations (5)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise