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Rabbit Recombinant Monoclonal CD93 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human, Rat samples. Cited in 5 publications.

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Images

Flow Cytometry (Intracellular) - Anti-CD93 antibody [EPR5386] (AB134079), expandable thumbnail
  • Western blot - Anti-CD93 antibody [EPR5386] (AB134079), expandable thumbnail
  • OI-RD Scanning - Anti-CD93 antibody [EPR5386] (AB134079), expandable thumbnail
  • Western blot - Anti-CD93 antibody [EPR5386] (AB134079), expandable thumbnail
  • Western blot - Anti-CD93 antibody [EPR5386] (AB134079), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-PFlow Cyt (Intra)ELISA
Human
Not recommended
Tested
Not recommended
Tested
Not recommended
Mouse
Not recommended
Predicted
Not recommended
Predicted
Not recommended
Rat
Not recommended
Tested
Not recommended
Expected
Not recommended

Not recommended
Not recommended

Species
Rat, Human, Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Species
Rat
Dilution info
1/1000 - 1/10000
Notes

-

Predicted
Predicted

Species
Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Rat, Human, Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100 - 1/500
Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Cell surface receptor that plays a role in various physiological processes including inflammation, phagocytosis, and cell adhesion. Plays a role in phagocytosis and enhances the uptake of apoptotic cells and immune complexes by acting as a receptor for defense collagens including surfactant protein A/SFTPA1, C1q, and mannose-binding lectin (MBL2) (PubMed:7977768). Plays a role in the regulation of endothelial cell function and adhesion by activating angiogenesis (PubMed:24809468). Mechanistically, exerts its angiogenic function by associating with beta-dystroglycan, leading to SRC-dependent phosphorylation and subsequent recruitment of CBL. In turn, CBL provides a docking site for downstream signaling components, such as CRKL to enhance cell migration (PubMed:26848865). Participates in angiogenesis also by acting as a receptor for the ECM pan-endothelial glycoprotein multimerin-2/MMRN2 and IGFBP7 ligands (PubMed:28671670, PubMed:36265539, PubMed:38218180). Both ligands play a non-redundant role in CD93-mediated endothelial cell function (PubMed:38218180). Acts as a key regulator of endothelial barrier function through modulating VEGFR2 function (By similarity).

Alternative names

CD93, C1QR1, MXRA4, Complement component C1q receptor, C1q/MBL/SPA receptor, CDw93, Complement component 1 q subcomponent receptor 1, Matrix-remodeling-associated protein 4, C1qR, C1qR(p), C1qRp

Recommended products

Rabbit Recombinant Monoclonal CD93 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human, Rat samples. Cited in 5 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR5386
Purification technique
Affinity purification Protein A
Dissociation constant
1.8 x 10-11 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

CD93 also known as C1q receptor and a type I transmembrane glycoprotein weighs approximately 68 kDa. This protein is mainly expressed on platelets monocytes endothelial cells and some cell lines within the immune system. CD93 is known for engaging in cell adhesion and phagocytosis processes. It has a notable presence on the surface of circulating cells indicating its involvement in physiological interactions essential for cellular communication.

Biological function summary

CD93 contributes to cellular processes through its role in the clearance of apoptotic cells and immune surveillance. It also partakes in angiogenesis facilitating the growth of new blood vessels. CD93 often functions as part of a larger complex in binding with other molecules to support cellular communication. By modulating the immune response CD93 ensures efficient removal of cellular debris maintaining homeostasis and preventing autoimmune reactions.

Pathways

CD93 operates within the innate immune response and complement system pathways. It closely interacts with other proteins such as C1q which is part of the complement activation pathway. This interaction supports the clearance of immune complexes and cellular debris. By facilitating these processes CD93 aids in maintaining the balance between immune activation and tolerance.

Associated diseases and disorders

CD93 has associations with inflammatory diseases and cancer progression. In inflammatory diseases its role in the immune system implicates it in conditions characterized by excessive inflammation often relating to the misregulation of immune functions. As for cancer CD93's involvement in angiogenesis links it to tumor growth with connections seen alongside vascular endothelial growth factor (VEGF) pathways. These insights into CD93's role suggest its potential as a therapeutic target in treating related pathological states.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Flow Cytometry (Intracellular) - Anti-CD93 antibody [EPR5386] (ab134079), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-CD93 antibody [EPR5386] (ab134079)

    Intracellular flow cytometric analysis of permeabilized THP-1 cells labelling CD93 red with ab134079 at 1/100 and a rabbit IgG negative (green).

  • Western blot - Anti-CD93 antibody [EPR5386] (ab134079), expandable thumbnail

    Western blot - Anti-CD93 antibody [EPR5386] (ab134079)

    All lanes: Western blot - Anti-CD93 antibody [EPR5386] (ab134079) at 1/1000 dilution

    Lane 1: THP-1 lysate at 10 µg

    Lane 2: Rat kidney lysate at 10 µg

    Lane 3: Human platelet lysate at 10 µg

    Secondary

    All lanes: HRP labelled Goat anti-rabbit at 1/2000 dilution

    Predicted band size: 69 kDa

    Observed band size: 120 kDa

  • OI-RD Scanning - Anti-CD93 antibody [EPR5386] (ab134079), expandable thumbnail

    OI-RD Scanning - Anti-CD93 antibody [EPR5386] (ab134079)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Western blot - Anti-CD93 antibody [EPR5386] (ab134079), expandable thumbnail

    Western blot - Anti-CD93 antibody [EPR5386] (ab134079)

    Bands around 100kDa and 75kDa are full length CD93 with different level of glycosylation (PMID: 19603257). We are not sure the identity of bands below 70kDa.

    The expression profile observed in Raji and 293T is consistent with the literatures (PMID: 31182688, PMID:15459234).

    Negative control: Raji and 293T (PMID: 31182688, PMID:15459234).

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-CD93 antibody [EPR5386] (ab134079) at 1/1000 dilution

    Lane 1: U937 (human histiocytic lymphoma monocyte) whole cell lysate at 20 µg

    Lane 2: Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate at 20 µg

    Lane 3: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

    Lane 4: Rat spleen tissue lysate at 20 µg

    Lane 5: Mouse spleen tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 69 kDa

    Observed band size: 100 kDa, 50-80 kDa

    Exposure time: 180s

  • Western blot - Anti-CD93 antibody [EPR5386] (ab134079), expandable thumbnail

    Western blot - Anti-CD93 antibody [EPR5386] (ab134079)

    The expression profile observed in Raji and 293T is consistent with the literatures (PMID: 31182688, PMID:15459234).

    Negative control: Raji and 293T (PMID: 31182688, PMID:15459234).

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-CD93 antibody [EPR5386] (ab134079) at 1/1000 dilution

    Lane 1: U937 (human histiocytic lymphoma monocyte) whole cell lysate at 20 µg

    Lane 2: Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate at 20 µg

    Lane 3: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

    Lane 4: Rat spleen tissue lysate at 20 µg

    Lane 5: Mouse spleen tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 100 kDa, 50-80 kDa

    Exposure time: 180s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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