AB134079

Anti-CD93 antibody [EPR5386]

RabMAb
Recombinant
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(12 Publications)

Rabbit Recombinant Monoclonal CD93 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human, Rat samples. Cited in 12 publications.

View Alternative Names

CD93, C1QR1, MXRA4, Complement component C1q receptor, C1q/MBL/SPA receptor, CDw93, Complement component 1 q subcomponent receptor 1, Matrix-remodeling-associated protein 4, C1qR, C1qR(p), C1qRp

5 Images

Flow Cytometry (Intracellular) - Anti-CD93 antibody [EPR5386] (AB134079)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-CD93 antibody [EPR5386] (AB134079)

Intracellular flow cytometric analysis of permeabilized THP-1 cells labelling CD93 red with ab134079 at 1/100 and a rabbit IgG negative (green).

Unknown

Western blot - Anti-CD93 antibody [EPR5386] (AB134079)

All lanes:

Western blot - Anti-CD93 antibody [EPR5386] (ab134079) at 1/1000 dilution

Lane 1:

THP-1 lysate at 10 µg

Lane 2:

Rat kidney lysate at 10 µg

Lane 3:

Human platelet lysate at 10 µg

Secondary

All lanes:

HRP labelled Goat anti-rabbit at 1/2000 dilution

Predicted band size: 69 kDa

Observed band size: 120 kDa

false

Lab

Western blot - Anti-CD93 antibody [EPR5386] (AB134079)

The expression profile observed in Raji and 293T is consistent with the literatures (PMID : 31182688, PMID : 15459234).

Negative control : Raji and 293T (PMID : 31182688, PMID : 15459234).

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-CD93 antibody [EPR5386] (ab134079) at 1/1000 dilution

Lane 1:

U937 (human histiocytic lymphoma monocyte) whole cell lysate at 20 µg

Lane 2:

Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate at 20 µg

Lane 3:

293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 4:

Rat spleen tissue lysate at 20 µg

Lane 5:

Mouse spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 100 kDa,50-80 kDa

false

Exposure time: 180s

Supplier Data

Western blot - Anti-CD93 antibody [EPR5386] (AB134079)

Bands around 100kDa and 75kDa are full length CD93 with different level of glycosylation (PMID : 19603257). We are not sure the identity of bands below 70kDa.

The expression profile observed in Raji and 293T is consistent with the literatures (PMID : 31182688, PMID : 15459234).

Negative control : Raji and 293T (PMID : 31182688, PMID : 15459234).

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-CD93 antibody [EPR5386] (ab134079) at 1/1000 dilution

Lane 1:

U937 (human histiocytic lymphoma monocyte) whole cell lysate at 20 µg

Lane 2:

Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate at 20 µg

Lane 3:

293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 4:

Rat spleen tissue lysate at 20 µg

Lane 5:

Mouse spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 69 kDa

Observed band size: 100 kDa,50-80 kDa

false

Exposure time: 180s

OI-RD Scanning - Anti-CD93 antibody [EPR5386] (AB134079)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-CD93 antibody [EPR5386] (AB134079)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Carrier free

Anti-CD93 antibody [EPR5386] - BSA and Azide free
578 PE

PE Anti-CD93 antibody [EPR5386]
660 APC

APC Anti-CD93 antibody [EPR5386]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-CD93 antibody [EPR5386]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-CD93 antibody [EPR5386]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-CD93 antibody [EPR5386]
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-CD93 antibody [EPR5386]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-CD93 antibody [EPR5386]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5386

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100 - 1/500", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD93 also known as C1q receptor and a type I transmembrane glycoprotein weighs approximately 68 kDa. This protein is mainly expressed on platelets monocytes endothelial cells and some cell lines within the immune system. CD93 is known for engaging in cell adhesion and phagocytosis processes. It has a notable presence on the surface of circulating cells indicating its involvement in physiological interactions essential for cellular communication.

Biological function summary

CD93 contributes to cellular processes through its role in the clearance of apoptotic cells and immune surveillance. It also partakes in angiogenesis facilitating the growth of new blood vessels. CD93 often functions as part of a larger complex in binding with other molecules to support cellular communication. By modulating the immune response CD93 ensures efficient removal of cellular debris maintaining homeostasis and preventing autoimmune reactions.

Pathways

CD93 operates within the innate immune response and complement system pathways. It closely interacts with other proteins such as C1q which is part of the complement activation pathway. This interaction supports the clearance of immune complexes and cellular debris. By facilitating these processes CD93 aids in maintaining the balance between immune activation and tolerance.

CD93 has associations with inflammatory diseases and cancer progression. In inflammatory diseases its role in the immune system implicates it in conditions characterized by excessive inflammation often relating to the misregulation of immune functions. As for cancer CD93's involvement in angiogenesis links it to tumor growth with connections seen alongside vascular endothelial growth factor (VEGF) pathways. These insights into CD93's role suggest its potential as a therapeutic target in treating related pathological states.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cell surface receptor that plays a role in various physiological processes including inflammation, phagocytosis, and cell adhesion. Plays a role in phagocytosis and enhances the uptake of apoptotic cells and immune complexes by acting as a receptor for defense collagens including surfactant protein A/SFTPA1, C1q, and mannose-binding lectin (MBL2) (PubMed : 7977768). Plays a role in the regulation of endothelial cell function and adhesion by activating angiogenesis (PubMed : 24809468). Mechanistically, exerts its angiogenic function by associating with beta-dystroglycan, leading to SRC-dependent phosphorylation and subsequent recruitment of CBL. In turn, CBL provides a docking site for downstream signaling components, such as CRKL to enhance cell migration (PubMed : 26848865). Participates in angiogenesis also by acting as a receptor for the ECM pan-endothelial glycoprotein multimerin-2/MMRN2 and IGFBP7 ligands (PubMed : 28671670, PubMed : 36265539, PubMed : 38218180). Both ligands play a non-redundant role in CD93-mediated endothelial cell function (PubMed : 38218180). Acts as a key regulator of endothelial barrier function through modulating VEGFR2 function (By similarity).

See full target information CD93

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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:21768 PubMed40596517

2025

Diminished angiogenic capacity in the hippocampus compared to the cortex indicates regional vulnerability.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaojie Li,Nanjing Li,Yao He,Jingyang Zu,Qixing Zhong,Zhihui Zhong

Molecular neurodegeneration 19:95 PubMed39695808

2024

Microglial CD2AP deficiency exerts protection in an Alzheimer's disease model of amyloidosis.

Applications

Unspecified application

Species

Unspecified reactive species

Lingliang Zhang,Lingling Huang,Yuhang Zhou,Jian Meng,Liang Zhang,Yunqiang Zhou,Naizhen Zheng,Tiantian Guo,Shanshan Zhao,Zijie Wang,Yuanhui Huo,Yingjun Zhao,Xiao-Fen Chen,Honghua Zheng,David M Holtzman,Yun-Wu Zhang

Scientific reports 14:13329 PubMed38858476

2024

Author Correction: The impact understanding of exosome therapy in COVID-19 and preparations for the future approaches in dealing with infectious diseases and inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Zeynab Nasiri,Hoorieh Soleimanjahi,Nafiseh Baheiraei,Seyed Mahmoud Hashemi,Mahmoud Reza Pourkarim

Journal of biochemical and molecular toxicology 38:e23688 PubMed38511888

2024

CD93 regulates breast cancer growth and vasculogenic mimicry through the PI3K/AKT/SP2 signaling pathway activated by integrin β1.

Applications

Unspecified application

Species

Unspecified reactive species

Hong Liu,Jianhui Zhang,Yanjun Zhao,Zhixiong Fan,Yongheng Yang,Yuanyuan Mao,Jingyuan Yang,Shungao Ma

Scientific reports 14:5724 PubMed38459174

2024

The impact understanding of exosome therapy in COVID-19 and preparations for the future approaches in dealing with infectious diseases and inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Zeynab Nasiri,Hoorieh Soleimanjahi,Nafiseh Baheiraei,Seyed Mahmoud Hashemi,Mahmoud Reza Pourkarim

The Journal of neuroscience : the official journal of the Society for Neuroscience 42:4958-4979 PubMed35606143

2022

Microglial Deficiency Impairs Phagocytosis of Synapse and Leads to Autism-Like Behaviors in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Meng,Linkun Han,Naizhen Zheng,Ting Wang,Hui Xu,Yiru Jiang,Zijie Wang,Zhaoji Liu,Qiuyang Zheng,Xian Zhang,Hong Luo,Dan Can,Jinsheng Lu,Huaxi Xu,Yun-Wu Zhang

Kidney international 101:137-143 PubMed34619231

2021

Highly multiplexed immunofluorescence of the human kidney using co-detection by indexing.

Applications

mIHC

Species

Human

Elizabeth K Neumann,Nathan Heath Patterson,Emilio S Rivera,Jamie L Allen,Maya Brewer,Mark P deCaestecker,Richard M Caprioli,Agnes B Fogo,Jeffrey M Spraggins

STAR protocols 2:100747 PubMed34430920

2021

Protocol for multimodal analysis of human kidney tissue by imaging mass spectrometry and CODEX multiplexed immunofluorescence.

Applications

mIHC

Species

Human

Elizabeth K Neumann,Nathan Heath Patterson,Jamie L Allen,Lukasz G Migas,Haichun Yang,Maya Brewer,David M Anderson,Jennifer Harvey,Danielle B Gutierrez,Raymond C Harris,Mark P deCaestecker,Agnes B Fogo,Raf Van de Plas,Richard M Caprioli,Jeffrey M Spraggins

Cell reports 34:108663 PubMed33503440

2021

Metoclopramide treatment blocks CD93-signaling-mediated self-renewal of chronic myeloid leukemia stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Carsten Riether,Ramin Radpour,Nils M Kallen,Damian T Bürgin,Chantal Bachmann,Christian M Schürch,Ursina Lüthi,Miroslav Arambasic,Sven Hoppe,Christoph E Albers,Gabriela M Baerlocher,Adrian F Ochsenbein

Oncology letters 18:6413-6422 PubMed31819775

2019

Radioimmunoimaging of I-labeled anti-CD93 monoclonal antibodies in a xenograft model of non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Weiwei Liu,Chao Zhang,Hui Cao,Dai Shi,Shanshan Zhao,Ting Liang,Guihua Hou

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD93 antibody [EPR5386]

Flow Cytometry (Intracellular) - Anti-CD93 antibody [EPR5386] (AB134079)

Western blot - Anti-CD93 antibody [EPR5386] (AB134079)

Western blot - Anti-CD93 antibody [EPR5386] (AB134079)

Western blot - Anti-CD93 antibody [EPR5386] (AB134079)

OI-RD Scanning - Anti-CD93 antibody [EPR5386] (AB134079)

Related conjugates and formulations (8)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (12)

Product promise