Rat Recombinant Monoclonal CD94 antibody. Suitable for Flow Cyt and reacts with Mouse samples.
IgG2a
Rat
pH: 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
ICC | IHC | Flow Cyt | |
---|---|---|---|
Mouse | Not recommended | Not recommended | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Mouse | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Immune receptor involved in self-nonself discrimination. In complex with KLRC1 or KLRC2 on cytotoxic and regulatory lymphocyte subsets, recognizes non-classical major histocompatibility (MHC) class Ib molecule MHC-E loaded with self-peptides derived from the signal sequence of classical MHC class Ia and non-classical MHC class Ib molecules. Enables cytotoxic cells to monitor the expression of MHC class I molecules in healthy cells and to tolerate self. Primarily functions as a ligand binding subunit as it lacks the capacity to signal.KLRD1-KLRC1 acts as an immune inhibitory receptor. Key inhibitory receptor on natural killer (NK) cells that regulates their activation and effector functions. Dominantly counteracts T cell receptor signaling on a subset of memory/effector CD8-positive T cells as part of an antigen-driven response to avoid autoimmunity. On intraepithelial CD8-positive gamma-delta regulatory T cells triggers TGFB1 secretion, which in turn limits the cytotoxic programming of intraepithelial CD8-positive alpha-beta T cells, distinguishing harmless from pathogenic antigens. In MHC-E-rich tumor microenvironment, acts as an immune inhibitory checkpoint and may contribute to progressive loss of effector functions of NK cells and tumor-specific T cells, a state known as cell exhaustion. Upon MHC-E-peptide binding, transmits intracellular signals through KLRC1 immunoreceptor tyrosine-based inhibition motifs (ITIMs) by recruiting INPP5D/SHIP-1 and INPPL1/SHIP-2 tyrosine phosphatases to ITIMs, and ultimately opposing signals transmitted by activating receptors through dephosphorylation of proximal signaling molecules.KLRD1-KLRC2 acts as an immune activating receptor. On cytotoxic lymphocyte subsets recognizes MHC-E loaded with signal sequence-derived peptides from non-classical MHC class Ib MHC-G molecules, likely playing a role in the generation and effector functions of adaptive NK cells and in maternal-fetal tolerance during pregnancy. Regulates the effector functions of terminally differentiated cytotoxic lymphocyte subsets, and in particular may play a role in adaptive NK cell response to viral infection. Upon MHC-E-peptide binding, transmits intracellular signals via the adapter protein TYROBP/DAP12, triggering the phosphorylation of proximal signaling molecules and cell activation.
Cd94, Cd94, Klrd1, Natural killer cells antigen CD94, Killer cell lectin-like receptor subfamily D member 1
Rat Recombinant Monoclonal CD94 antibody. Suitable for Flow Cyt and reacts with Mouse samples.
IgG2a
Rat
pH: 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
18D3
Affinity purification Protein A
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
This supplementary information is collated from multiple sources and compiled automatically.
CD94 also known as killer cell lectin-like receptor subfamily D member 1 (KLRD1) plays a significant mechanical role in the immune system. This protein forms part of a receptor family involved in natural killer (NK) cell function. CD94 typically assembles on the cell surface as a heterodimer often pairing with NKG2 proteins. Weighing approximately 30 kDa CD94 mainly exhibits expression on NK cells and a subset of T cells where it contributes to immune regulation.
CD94 is essential in recognizing and binding to non-classical MHC class I molecules when it pairs with NKG2 molecules such as NKG2A or NKG2C. This dimer forms a complex receptor which plays an important role in modulating NK cell activity. Through this receptor complex CD94 influences the immune response by either inhibiting or activating NK cells depending on its NKG2 partner. This regulation helps maintain a balanced immune response preventing excessive activation and autoimmunity.
CD94 interacts with the signaling pathways related to the immune response. In particular it is involved in the natural killer cell lectin-like receptor signaling pathway. This pathway manages the activation and inhibition of NK cells integrating signals received through various receptors including CD94/NKG2 dimers. Associated proteins like DAP12 may amplify these signals which ultimately influence cytotoxic activities and cytokine production.
CD94 has notable connections with conditions such as cancer and viral infections. For example certain tumors manipulate CD94 expression on NK cells to evade immune surveillance. Additionally alterations in CD94 and its partner molecules like NKG2A have been observed in viral infections possibly affecting how the immune system responds. Understanding CD94's relationship with these diseases can offer insights into potential therapeutic strategies targeting immune regulation.
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We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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Terms & Conditions.
Flow cytometric analysis of Mouse spleen cells cells labelling CD94 with abab21650 at 1/1000 dilution (0.1ug)/ Right compared with a Rat monoclonal IgG / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 488, Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor® 488) preadsorbed ab150161) at 1/2000 dilution was used as the secondary antibody. Cells were stained with rat IgG or ab21650. Then stained with goat anti-rat IgG Fc secondary antibody. Prior to data acquisition, anti-NK1.1 conjugated to Alexa Fluor® 647 was stained. Gated on viable cells.
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