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AB255696

Anti-CD96 antibody [EPR22120-174] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal CD96 antibody. Carrier free. Suitable for Flow Cyt and reacts with Human samples. Cited in 1 publication.

View Alternative Names

CD96, T-cell surface protein tactile, Cell surface antigen CD96, T cell-activated increased late expression protein

1 Images
Flow Cytometry - Anti-CD96 antibody [EPR22120-174] - BSA and Azide free (AB255696)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-CD96 antibody [EPR22120-174] - BSA and Azide free (AB255696)

Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) cells labelling CD96 with ab224687 at 1/60 dilution (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left).

Goat anti rabbit IgG (Alexa Fluor® 488, ab150097) at 1/5000 dilution was used as the secondary antibody.

Cells were stained with rabbit IgG (Left) or ab224687 (Right). Then stained with anti-CD3 conjugated to Alexa Fluor® 647.

Gated on viable cells.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224687).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22120-174

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

ab255696 is the carrier-free version of ab224687.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD96 also known as TACTILE is an immunoglobulin-like protein weighing approximately 65 kDa. This protein is expressed mostly on T cells and natural killer (NK) cells with its presence also noted on certain leukemic cells. CD96 functions mechanistically as an adhesion molecule mediating interaction between cells significantly involving in immune response modulation. Through binding proteins like CD155 it regulates immune cell migration and activity playing a significant role in cellular communication within the immune system.
Biological function summary

The CD96 protein contributes to the immune synapse formation by interacting with other cell surface and intracellular proteins. It is not directly part of a multiprotein complex but closely interacts with the receptor-ligand pairs within immune cell networks. It plays a role in the control of NK cell activity and helps maintain immune homeostasis by balancing activation and inhibition cues.

Pathways

The CD96 protein significantly impacts immunological pathways particularly those involving T cell and NK cell activation. It connects with pathways such as the DNAM-1 (CD226) and CD155 axis influencing immune cell recognition and response. These pathways regulate cellular activities like cytotoxicity and cytokine production where CD96 collaborates with proteins like DNAM-1 and TIGIT to fine-tune immune responses.

CD96 is closely associated with cancer and autoimmune diseases. Its expression level and functional role in cell adhesion and immune response modulation affect tumor immune evasion and surveillance often serving as a potential therapeutic target in cancer immunotherapy. Moreover its interactions with CD155 and DNAM-1 suggest its involvement in autoimmune conditions where regulation of immune cell activity becomes dysregulated pointing towards its relevance in diseases like rheumatoid arthritis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May be involved in adhesive interactions of activated T and NK cells during the late phase of the immune response. Promotes NK cell-target adhesion by interacting with PVR present on target cells. May function at a time after T and NK cells have penetrated the endothelium using integrins and selectins, when they are actively engaging diseased cells and moving within areas of inflammation.
See full target information CD96
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Acta neuropathologica communications 13:86 PubMed40307956

2025

Prion replication in organotypic brain slice cultures is distinct from in vivo inoculation and is species dependent.

Applications

Unspecified application

Species

Unspecified reactive species

Jessy A Slota,Lise Lamoureux,Jennifer Myskiw,Kathy L Frost,Sarah J Medina,Dominic M S Kielich,Melanie Leonhardt,Gunjan Thapar,Ben A Bailey-Elkin,Stephanie A Booth
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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