Anti-CD96 antibody [EPR26489-23] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD96 antibody [EPR26489-23] - BSA and Azide free (AB315219)

This data was developed using ab315218, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Panel A : HEK-293T cells transfected with a human CD96 expression vector containing a his tag. Panel B : HEK-293T cells transfected with empty vector containing a his tag. tissue labeling CD96 with ab315218 at 1/100 (4.96 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on (A) HEK-293T transfected with a human CD96 expression vector, no staining on (B) HEK-293T transfected with empty vector. The section was incubated with ab315218 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD96 antibody [EPR26489-23] - BSA and Azide free (AB315219)

This data was developed using ab315218, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling CD96 with ab315218 at 1/100 (4.96 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on human cerebrum. The section was incubated with ab315218 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD96 antibody [EPR26489-23] - BSA and Azide free (AB315219)

This data was developed using ab315218, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human NK/T lymphoma tissue labeling CD96 with ab315218 at 1/100 (4.96 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human NK/T lymphoma. The section was incubated with ab315218 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD96 antibody [EPR26489-23] - BSA and Azide free (AB315219)

This data was developed using ab315218, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human thymoma tissue labeling CD96 with ab315218 at 1/100 (4.96 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human thymoma. The section was incubated with ab315218 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD96 antibody [EPR26489-23] - BSA and Azide free (AB315219)

This data was developed using ab315218, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human endometrium tissue labeling CD96 with ab315218 at 1/100 (4.96 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on immune cells of human endometrium. The section was incubated with ab315218 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IP

Supplier Data

Immunoprecipitation - Anti-CD96 antibody [EPR26489-23] - BSA and Azide free (AB315219)

This data was developed using ab315218, the same antibody clone in a different buffer formulation.

CD96 was immunoprecipitated from 0.35 mg A375 (human malignant melanoma epithelial cell) whole cell lysate with ab315218 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab315218 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : A375 (human malignant melanoma epithelial cell) whole cell lysate

Lane 2 : ab315218 IP in A375 (human malignant melanoma epithelial cell) whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab315218 in A375 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-CD96 antibody [EPR26489-23] (<a href='/en-us/products/primary-antibodies/cd96-antibody-epr26489-23-ab315218'>ab315218</a>) at 1/30 dilution

All lanes:

A375 (human malignant melanoma epithelial cell) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 24s

• WB

Supplier Data

Western blot - Anti-CD96 antibody [EPR26489-23] - BSA and Azide free (AB315219)

This data was developed using ab315218, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 1313846).

Negative control : Daudi (PMID : 1313846); MCF-7 (PMID : 1313846)

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-CD96 antibody [EPR26489-23] (<a href='/en-us/products/primary-antibodies/cd96-antibody-epr26489-23-ab315218'>ab315218</a>) at 1/1000 dilution

Lane 1:

A375 (human malignant melanoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

HuT-78 (human Sezary syndrome cutaneous T lymphocyte) whole cell lysate at 20 µg

Lane 3:

Daudi (human Burkitt's lymphoma lymphoblast) whole cell lysate at 20 µg

Lane 4:

MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 100 kDa,160 kDa,36 kDa

false

Exposure time: 48s