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AB248686

Anti-CD97 antibody [EPR4428] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal CD97 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

CD97, ADGRE5, Adhesion G protein-coupled receptor E5, Leukocyte antigen CD97

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD97 antibody [EPR4428] - BSA and Azide free (AB248686)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD97 antibody [EPR4428] - BSA and Azide free (AB248686)

This data was developed using ab134105, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human colonic adenocarcinoma tissue labelling CD97 with ab134105 antibody at a dilution of 1/1000.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-CD97 antibody [EPR4428] - BSA and Azide free (AB248686)
  • WB

Lab

Western blot - Anti-CD97 antibody [EPR4428] - BSA and Azide free (AB248686)

This data was developed using ab134105, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : Lane 1 : 180 seconds; Lane 2 : 60 seconds.

The 28kDa is ß-chain. This is supported by many papers (PMID : 20084281, 18941248).

Lane 1:

Western blot - Anti-CD97 antibody [EPR4428] (<a href='/en-us/products/primary-antibodies/cd97-antibody-epr4428-ab134105'>ab134105</a>) at 1/10000 dilution

Lane 2:

Western blot - Anti-CD97 antibody [EPR4428] (<a href='/en-us/products/primary-antibodies/cd97-antibody-epr4428-ab134105'>ab134105</a>) at 1/20000 dilution

Lane 1:

U-937 (Human histiocytic lymphoma monocyte) whole cell lysate at 20 µg

Lane 2:

THP-1 (Human monocytic leukemia monocyte) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/20000 dilution

Predicted band size: 92 kDa

Observed band size: 28 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4428

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

ab248686 is the carrier-free version of ab134105.

Species reactivity
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD97 also known as adhesion G protein-coupled receptor E5 is a member of the EGF-TM7 subfamily of adhesion GPCRs. The protein has a mass of approximately 83-93 kDa and consists of distinct extracellular transmembrane and cytoplasmic domains. CD97 expresses highly on cells of the immune system especially granulocytes activated T-lymphocytes and smooth muscle cells. Researchers frequently link CD97 expression to immune cell activation and migration processes.
Biological function summary

CD97 plays a significant role in cell communication and adhesion. The protein is part of a multiprotein complex and interacts with chondroitin sulfate and other heparan sulfate proteoglycans. This interaction modulates inflammatory responses and tissue remodeling. CD97 influences immune responses by mediating cellular interaction and signaling through its long extracellular domain. Its activity is essential for leukocyte trafficking and extravasation.

Pathways

CD97 participates in important cell adhesion and immune system pathways. It interacts notably with the EGF-like crossover domains and the GPCR pathways. Related proteins such as CD55 also known as decay-accelerating factor interact with CD97 influencing the immune regulatory processes. These interactions enhance our understanding of leukocyte recruitment and the maintenance of tissue architecture during inflammatory responses.

CD97 is associated with autoimmune diseases and cancer. High CD97 expression levels relate to diseases like rheumatoid arthritis and certain types of tumors including gastric carcinoma. In cancer CD97 interacts with integrin molecules promoting metastasis and tumor invasion. Understanding CD97's connection to integrins and other components could provide insights into therapeutic targets for autoimmune and cancer treatment.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor potentially involved in both adhesion and signaling processes early after leukocyte activation. Plays an essential role in leukocyte migration.
See full target information ADGRE5
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Theranostics 12:4513-4535 PubMed35832075

2022

Blockade of phosphotyrosine pathways suggesting SH2 superbinder as a novel therapy for pulmonary fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Meng Wang,An-Dong Liu,Qian Niu,Xiao Feng,Yuan-Yi Zheng,Shuai-Jun Chen,Hui Xu,Qian Li,Guo-Qing Hou,Xiao-Yang Bi,Yu-Zhi Lu,Pei-Pei Cheng,Li-Mei Liang,Ye-Han Jiang,Li-Qin Zhao,Fei Liu,Lin-Jie Song,Li-Ling Zhou,Ling-Yan Xiao,Feng Chen,Shawn Shun-Cheng Li,Wan-Li Ma,Xuan Cao,Hong Ye
View all publications
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Product promise

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