Knockout Tested Rabbit Recombinant Monoclonal CD98 antibody. Carrier free. Suitable for IP, Flow Cyt, ICC/IF, IHC-P, WB and reacts with Human samples.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: 100% PBS
Liquid
Monoclonal
IP | Flow Cyt | ICC/IF | IHC-P | WB | |
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Human | Tested | Tested | Tested | Tested | Tested |
Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
Species | Dilution info | Notes |
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Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Rat, Mouse | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Rat, Mouse | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Rat, Mouse | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
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Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
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Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Rat, Mouse | Dilution info - | Notes - |
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Acts as a chaperone that facilitates biogenesis and trafficking of functional transporters heterodimers to the plasma membrane. Forms heterodimer with SLC7 family transporters (SLC7A5, SLC7A6, SLC7A7, SLC7A8, SLC7A10 and SLC7A11), a group of amino-acid antiporters (PubMed:10574970, PubMed:10903140, PubMed:11557028, PubMed:30867591, PubMed:33298890, PubMed:33758168, PubMed:34880232, PubMed:9751058, PubMed:9829974, PubMed:9878049). Heterodimers function as amino acids exchangers, the specificity of the substrate depending on the SLC7A subunit. Heterodimers SLC3A2/SLC7A6 or SLC3A2/SLC7A7 mediate the uptake of dibasic amino acids (PubMed:10903140, PubMed:9829974). Heterodimer SLC3A2/SLC7A11 functions as an antiporter by mediating the exchange of extracellular anionic L-cystine and intracellular L-glutamate across the cellular plasma membrane (PubMed:34880232). SLC3A2/SLC7A10 translocates small neutral L- and D-amino acids across the plasma membrane (By similarity). SLC3A2/SLC75 or SLC3A2/SLC7A8 translocates neutral amino acids with broad specificity, thyroid hormones and L-DOPA (PubMed:10574970, PubMed:11389679, PubMed:11557028, PubMed:11564694, PubMed:11742812, PubMed:12117417, PubMed:12225859, PubMed:12716892, PubMed:15980244, PubMed:30867591, PubMed:33298890, PubMed:33758168). SLC3A2 is essential for plasma membrane localization, stability, and the transport activity of SLC7A5 and SLC7A8 (PubMed:10391915, PubMed:10574970, PubMed:11311135, PubMed:15769744, PubMed:33066406). When associated with LAPTM4B, the heterodimer SLC7A5 is recruited to lysosomes to promote leucine uptake into these organelles, and thereby mediates mTORC1 activation (PubMed:25998567). Modulates integrin-related signaling and is essential for integrin-dependent cell spreading, migration and tumor progression (PubMed:11121428, PubMed:15625115).(Microbial infection) In case of hepatitis C virus/HCV infection, the complex formed by SLC3A2 and SLC7A5/LAT1 plays a role in HCV propagation by facilitating viral entry into host cell and increasing L-leucine uptake-mediated mTORC1 signaling activation, thereby contributing to HCV-mediated pathogenesis.(Microbial infection) Acts as a receptor for malaria parasite Plasmodium vivax (Thai isolate) in immature red blood cells.
MDU1, SLC3A2, MDU1, Amino acid transporter heavy chain SLC3A2, 4F2 cell-surface antigen heavy chain, 4F2 heavy chain antigen, Lymphocyte activation antigen 4F2 large subunit, Solute carrier family 3 member 2, 4F2hc
Knockout Tested Rabbit Recombinant Monoclonal CD98 antibody. Carrier free. Suitable for IP, Flow Cyt, ICC/IF, IHC-P, WB and reacts with Human samples.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: 100% PBS
Liquid
Monoclonal
Yes
EPR27110-42
Affinity purification Protein A
Blue Ice
+4°C
+4°C
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This supplementary information is collated from multiple sources and compiled automatically.
CD98 also known as CD98hc CD98 protein or LAT1/CD98 is a type II membrane protein often found in association with the heavy chains of amino acid transporters like LAT1. It has a molecular mass of approximately 85-100 kDa. CD98 is widely expressed in numerous tissues including the liver kidney and various tumors. It plays an important role in cellular processes by facilitating the transport of amino acids across plasma membranes which is essential for maintaining cellular homeostasis and proliferation.
CD98 forms a complex with light chains of the heterodimeric amino acid transporters such as LAT1 and LAT2. This interaction enhances the uptake of neutral amino acids into cells. CD98 also interacts with integrins influencing cell adhesion and migration which are critical for cellular signaling and response. Through these interactions CD98 impacts not only cell survival and growth but also immune responses and angiogenesis.
CD98 is a vital component of the mTOR signaling pathway influencing cell growth and proliferation. It also plays a part in the integrin signaling pathway which regulates cell adhesion and migration. In these pathways CD98 works closely with proteins like FG1 and mTORC1. These relationships enable it to modulate cellular responses to nutritional availability and mechanical cues from the environment.
CD98 expression and function have links to cancer and autoimmune diseases. High expression levels of CD98 are observed in various cancers such as lymphomas where it correlates with increased tumor growth and poor prognosis. In autoimmune disorders like rheumatoid arthritis CD98 influences immune cell activation and migration. Proteins such as CP1 and FG1 interact with CD98 contributing to disease pathology by affecting cellular and immune responses.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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Terms & Conditions.
This data was developed using Anti-CD98 antibody [EPR27110-42] ab307587, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human colon carcinom tissue labeling CD98 with Anti-CD98 antibody [EPR27110-42] ab307587 at 1/5000 (0.099 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human colon carcinoma (PMID: 29860836). The section was incubated with Anti-CD98 antibody [EPR27110-42] ab307587 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument.Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 10 mins
This data was developed using Anti-CD98 antibody [EPR27110-42] ab307587, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized SLC3A2 (CD98) KO HeLa (SLC3A2 (CD98) knockout human cervix adenocarcinoma epithelial cell) (Human SLC3A2 (CD98) knockout HeLa cell line ab265708) cells labelling CD98 with Anti-CD98 antibody [EPR27110-42] ab307587 at 1/100 (4.97 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/mL dilution (Green). Confocal image showing membrane and weak cytoplasmic staining in parental HeLa cell line.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.
This data was developed using Anti-CD98 antibody [EPR27110-42] ab307587, the same antibody clone in a different buffer formulation.
CD98 was immunoprecipitated from 0.35 mg HeLa whole cell lysate with Anti-CD98 antibody [EPR27110-42] ab307587 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-CD98 antibody [EPR27110-42] ab307587 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2: Anti-CD98 antibody [EPR27110-42] ab307587 IP in HeLa whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-CD98 antibody [EPR27110-42] ab307587 in hela whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second
All lanes: Immunoprecipitation - Anti-CD98 antibody [EPR27110-42] (Anti-CD98 antibody [EPR27110-42] ab307587) at 1/30 dilution
All lanes: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 1s
CD98 was immunoprecipitated from 0.35 mg HeLa whole cell lysate with Anti-CD98 antibody [EPR27110-42] ab307587 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-CD98 antibody [EPR27110-42] ab307587 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2: Anti-CD98 antibody [EPR27110-42] ab307587 IP in HeLa whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-CD98 antibody [EPR27110-42] ab307587 in hela whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second
This data was developed using Anti-CD98 antibody [EPR27110-42] ab307587, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Low expression: SH-SY5Y
Exposure time: 3 seconds
All lanes: Western blot - Anti-CD98 antibody [EPR27110-42] (Anti-CD98 antibody [EPR27110-42] ab307587) at 1/1000 dilution
Lane 1: Wild-type HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: SLC3A2 (CD98) knockout HeLa whole cell lysate at 20 µg
Lane 3: HeLa whole cell lysate at 20 µg
Lane 4: K562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg
Lane 5: SW620 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 6: SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 70-130 kDa
Exposure time: 3s
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Low expression: SH-SY5Y
Exposure time: 3 seconds
This data was developed using Anti-CD98 antibody [EPR27110-42] ab307587, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling CD98 with Anti-CD98 antibody [EPR27110-42] ab307587 at 1/100 (4.97 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/mL dilution (Green). Confocal image showing membrane and weak cytoplasmic staining in HeLa cell line.Low expression: SH-SY5YImage was taken with a confocal microscope(Leica-Microsystems, TCS SP8). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.
This data was developed using Anti-CD98 antibody [EPR27110-42] ab307587, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling CD98 with Anti-CD98 antibody [EPR27110-42] ab307587 at 1/5000 (0.099 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human kidney. The section was incubated with Anti-CD98 antibody [EPR27110-42] ab307587 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 10 mins
This data was developed using Anti-CD98 antibody [EPR27110-42] ab307587, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Low expression: skeletal muscle (PMID: 10574970, PMID: 11557028).
The lanes 2 and 3 were developed using a high sensitivity ECL substrate.
Exposure time: Lane 1: 6 seconds, lanes 2 and 3: 48 seconds.
All lanes: Western blot - Anti-CD98 antibody [EPR27110-42] (Anti-CD98 antibody [EPR27110-42] ab307587) at 1/1000 dilution
Lane 1: Human kidney tissue lysate at 20 µg
Lane 2: Human breast tissue lysate at 20 µg
Lane 3: Human skeletal muscle tissue lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Observed band size: 70-130 kDa
Exposure time: 6s
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Low expression: skeletal muscle (PMID: 10574970, PMID: 11557028).
The lanes 2 and 3 were developed using a high sensitivity ECL substrate.
Exposure time: Lane 1: 6 seconds, lanes 2 and 3: 48 seconds.
This data was developed using Anti-CD98 antibody [EPR27110-42] ab307587, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling CD98 with Anti-CD98 antibody [EPR27110-42] ab307587 at 1/5000 (0.099 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on islet of human pancreas. The section was incubated with Anti-CD98 antibody [EPR27110-42] ab307587 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument.Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 10 mins
This data was developed using Anti-CD98 antibody [EPR27110-42] ab307587, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of Wild-type HeLa (human cervix adenocarcinoma epithelial cell, Right) / SLC3A2 (CD98) knockout HeLa (Left) cells labelling CD98 with Anti-CD98 antibody [EPR27110-42] ab307587 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti-Rabbit IgG (Alexa Fluor® 647, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed ab150083) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.Positive staining on HeLa cells (Human wild-type HeLa cell line ab255928), while no staining on CD98 knockout HeLa cells (Human SLC3A2 (CD98) knockout HeLa cell line ab265708).
This data was developed using Anti-CD98 antibody [EPR27110-42] ab307587, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of SH-SY5Y (human neuroblastoma epithelial cell, Left) / HeLa (human cervix adenocarcinoma epithelial cell, Right) cells labelling CD98 with Anti-CD98 antibody [EPR27110-42] ab307587 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.Low expression: SH-SY5Y .
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