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AB27271

Anti-CD99 antibody

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(6 Publications)

Rabbit Polyclonal CD99 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 6 publications.

View Alternative Names

CD99, MIC2, MIC2X, MIC2Y, CD99 antigen, 12E7, E2 antigen, Protein MIC2, T-cell surface glycoprotein E2

1 Images
Western blot - Anti-CD99 antibody (AB27271)
  • WB

Unknown

Western blot - Anti-CD99 antibody (AB27271)

All lanes:

Western blot - Anti-CD99 antibody (ab27271) at 1 µg/mL

Lane 1:

Human thymus tissue lysate - total protein (<a href='/en-us/products/unavailable/human-thymus-tissue-lysate-total-protein-ab30146'>ab30146</a>) at 10 µg

Lane 2:

Human testis tissue lysate - total protein (<a href='/en-us/products/unavailable/human-testis-tissue-lysate-total-protein-ab30257'>ab30257</a>) at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 19 kDa

Observed band size: 32 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Alternative Products

Primary Antibodies

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD99 antibody [EPR3097Y] (AB75858)

  • 0
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Primary Antibodies

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Primary Antibodies

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Anti-CD99 antibody [EPR3096] - BSA and Azide free

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Flow Cytometry - Anti-CD99 antibody [EPR3096] - BSA and Azide free (AB226021)

  • 0
  • 0
Primary Antibodies

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD99 antibody [SP119] - BSA and Azide free (AB240990)

  • 0
  • 0
Primary Antibodies

AB108297

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RabMAb|Recombinant

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Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Primary Antibodies

AB10558

Anti-CD45 antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody (AB10558)

  • 4
  • 62
Primary Antibodies

AB955

Anti-CD68 antibody [KP1]

Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1] (AB955)

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  • 48
Primary Antibodies

AB64088

Anti-CD20 antibody [SP32]

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [SP32] (AB64088)

  • 5
  • 12
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
This antibody is affinity purified.
Storage buffer
pH: 7.6 Preservative: 0.08% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD99 also known as MIC2 or HO36 is a transmembrane protein of approximately 32-35 kDa. Expressed mainly on the surface of human cells CD99 plays a role in several physiological processes. It is prominently found on T cells B cells granulocytes and some capillary endothelial cells. The protein interacts with homophilic binding partners and influences various cellular functions depending on its location and cellular context. Immunohistochemistry for CD99 (CD99 IHC) is commonly used in research and diagnostic settings to study its involvement and presence in tissues.
Biological function summary

CD99 functions as a modulator in cell adhesion and migration. It facilitates the separation of leukocytes from the endothelium during transendothelial migration impacting the immune response and inflammation. CD99 is not part of a complex itself but interacts with extracellular matrix (ECM) components to perform its role. It contributes to the regulation of cell death pathways showcasing its versatility in maintaining cellular homeostasis.

Pathways

CD99 primarily participates in the regulation of T-cell adhesion and transmigration pathways. It supports the E-selectin and integrins pathway which is important for T-cell migration to sites of inflammation. CD99 works in conjunction with proteins like ICAM-1 and VCAM-1 assisting these proteins in facilitating immune cell movement and interactions. Its involvement in these pathways highlights its significance in immune surveillance and response modulation.

CD99 shows a notable connection with Ewing sarcoma and T-cell acute lymphoblastic leukemia (T-ALL). It is highly expressed in Ewing sarcoma a malignant bone tumor and can aid in diagnostic identification alongside other markers such as HOXA9. Furthermore its expression profiles in T-ALL patients suggest a potential role in the progression or maintenance of the disease. CD99's interactions with these malignancies make it a potential target in therapeutic research exploring pathways and molecules that might modulate its expression or function.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Involved in T-cell adhesion processes (By similarity).
See full target information CD99
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Clinical cancer research : an official journal of the American Association for Cancer Research 29:3541-3553 PubMed37279093

2023

A Bifunctional PARP-HDAC Inhibitor with Activity in Ewing Sarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Louise Ramos,Sarah Truong,Beibei Zhai,Jay Joshi,Fariba Ghaidi,Michael M Lizardo,Taras Shyp,Sonia H Y Kung,Alireza M Rezakhanlou,Htoo Zarni Oo,Hans Adomat,Stephane Le Bihan,Colin Collins,Jeffrey Bacha,Dennis Brown,John Langlands,Wang Shen,Nada Lallous,Poul H Sorensen,Mads Daugaard

Frontiers in oncology 11:645757 PubMed33828989

2021

NSG Mice Facilitate Characterization of Ewing Sarcoma Lung Metastasis Using the PuMA Model.

Applications

Unspecified application

Species

Unspecified reactive species

Renata Scopim-Ribeiro,Michael M Lizardo,Hai-Feng Zhang,Anne-Chloé Dhez,Chistopher S Hughes,Poul H Sorensen

Frontiers in immunology 10:651 PubMed31001265

2019

Targeting Tumor Vascular CD99 Inhibits Tumor Growth.

Applications

Unspecified application

Species

Unspecified reactive species

Elisabeth J M Huijbers,Inge M van der Werf,Lisette D Faber,Lena D Sialino,Pia van der Laan,Hanna A Holland,Anca M Cimpean,Victor L J L Thijssen,Judy R van Beijnum,Arjan W Griffioen

The American journal of surgical pathology 35:1749-50 PubMed21997696

2011

Paranuclear dot-like immunostaining for CD99: presence in colonic adenomas and adenocarcinomas.

Applications

Unspecified application

Species

Unspecified reactive species

Rami Makhoul,Arnold M Schwartz,Russell Williams,Norman H Lee

Clinical cancer research : an official journal of the American Association for Cancer Research 17:56-66 PubMed21047978

2010

Ewing tumors that do not overexpress BMI-1 are a distinct molecular subclass with variant biology: a report from the Children's Oncology Group.

Applications

IHC-P

Species

Human

Aaron Cooper,John van Doorninck,Lingyun Ji,Darren Russell,Marc Ladanyi,Hiroyuki Shimada,Mark Krailo,Richard B Womer,Jessie Hao-ru Hsu,Dafydd Thomas,Timothy J Triche,Richard Sposto,Elizabeth R Lawlor

Oncogene 28:4126-32 PubMed19718047

2009

GSTM4 is a microsatellite-containing EWS/FLI target involved in Ewing's sarcoma oncogenesis and therapeutic resistance.

Applications

IHC

Species

Human

W Luo,K Gangwal,S Sankar,K M Boucher,D Thomas,S L Lessnick
View all publications
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Product promise

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