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AB23617

Anti-CD99 antibody [DN16]

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(4 Publications)

Mouse Monoclonal CD99 antibody. Suitable for Flow Cyt, IHC-Fr and reacts with Human samples. Cited in 4 publications.

View Alternative Names

CD99, MIC2, MIC2X, MIC2Y, CD99 antigen, 12E7, E2 antigen, Protein MIC2, T-cell surface glycoprotein E2

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

DN16

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-Fr, Flow Cyt

applications

Epitope

Epitope analysis of the DN16 clone suggests the antibody recognizes a minimal peptide sequence 'LPDNENKK' located between residues 32 and 39 towards the N-terminal region of the molecule. This sequence is present in both isoforms I and II but is largely absent from isoform 3 suggesting that the antibody will only recognize isoforms I and II (Gil et al. 2002).

Specificity

ab23617 recognises the human CD99 cell surface antigen, a 32kD glycoprotein expressed by all leucocytes. CD99 expression tends to be highest on immature cells.

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Alternative Products

Primary Antibodies

AB208372

Alexa Fluor® 488 Anti-CD99 antibody [EPR3096]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-CD99 antibody [EPR3096] (AB208372)

  • 0
  • 0
Primary Antibodies

AB75858

Anti-CD99 antibody [EPR3097Y]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD99 antibody [EPR3097Y] (AB75858)

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Primary Antibodies

AB226021

Anti-CD99 antibody [EPR3096] - BSA and Azide free

RabMAb|Recombinant

Flow Cytometry - Anti-CD99 antibody [EPR3096] - BSA and Azide free (AB226021)

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Primary Antibodies

AB240990

Anti-CD99 antibody [SP119] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD99 antibody [SP119] - BSA and Azide free (AB240990)

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Primary Antibodies

AB108297

Anti-CD99 antibody [EPR3096]

RabMAb|Recombinant

Flow Cytometry - Anti-CD99 antibody [EPR3096] (AB108297)

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Complementary Products

Assay Kits

AB309394

Human CD99 ELISA Kit

Sandwich ELISA - Human CD99 ELISA Kit (AB309394)

  • 0
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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
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Primary Antibodies

AB10558

Anti-CD45 antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody (AB10558)

  • 4
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Primary Antibodies

AB955

Anti-CD68 antibody [KP1]

Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1] (AB955)

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Primary Antibodies

AB64088

Anti-CD20 antibody [SP32]

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [SP32] (AB64088)

  • 5
  • 12
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD99 also known as MIC2 or HO36 is a transmembrane protein of approximately 32-35 kDa. Expressed mainly on the surface of human cells CD99 plays a role in several physiological processes. It is prominently found on T cells B cells granulocytes and some capillary endothelial cells. The protein interacts with homophilic binding partners and influences various cellular functions depending on its location and cellular context. Immunohistochemistry for CD99 (CD99 IHC) is commonly used in research and diagnostic settings to study its involvement and presence in tissues.
Biological function summary

CD99 functions as a modulator in cell adhesion and migration. It facilitates the separation of leukocytes from the endothelium during transendothelial migration impacting the immune response and inflammation. CD99 is not part of a complex itself but interacts with extracellular matrix (ECM) components to perform its role. It contributes to the regulation of cell death pathways showcasing its versatility in maintaining cellular homeostasis.

Pathways

CD99 primarily participates in the regulation of T-cell adhesion and transmigration pathways. It supports the E-selectin and integrins pathway which is important for T-cell migration to sites of inflammation. CD99 works in conjunction with proteins like ICAM-1 and VCAM-1 assisting these proteins in facilitating immune cell movement and interactions. Its involvement in these pathways highlights its significance in immune surveillance and response modulation.

CD99 shows a notable connection with Ewing sarcoma and T-cell acute lymphoblastic leukemia (T-ALL). It is highly expressed in Ewing sarcoma a malignant bone tumor and can aid in diagnostic identification alongside other markers such as HOXA9. Furthermore its expression profiles in T-ALL patients suggest a potential role in the progression or maintenance of the disease. CD99's interactions with these malignancies make it a potential target in therapeutic research exploring pathways and molecules that might modulate its expression or function.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Involved in T-cell adhesion processes (By similarity).
See full target information CD99
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 20: PubMed30845661

2019

CD99 Expression in Glioblastoma Molecular Subtypes and Role in Migration and Invasion.

Applications

Unspecified application

Species

Unspecified reactive species

Lais C Cardoso,Roseli da S Soares,Talita de S Laurentino,Antonio M Lerario,Suely K N Marie,Sueli Mieko Oba-Shinjo

Clinical cancer research : an official journal of the American Association for Cancer Research 20:678-87 PubMed24218512

2013

Targeted imaging of Ewing sarcoma in preclinical models using a 64Cu-labeled anti-CD99 antibody.

Applications

Unspecified application

Species

Unspecified reactive species

Allison F O'Neill,Jason L J Dearling,Yuchuan Wang,Tanya Tupper,Yanping Sun,Jon C Aster,Monica L Calicchio,Antonio R Perez-Atayde,Alan B Packard,Andrew L Kung

Rheumatology (Oxford, England) 48:734-40 PubMed19439502

2009

The proadhesive phenotype of systemic sclerosis skin promotes myeloid cell adhesion via ICAM-1 and VCAM-1.

Applications

ELISA, IHC-Fr

Species

Human, Human

Bradley J Rabquer,Yong Hou,Francesco Del Galdo,G Kenneth Haines,Michele L Gerber,Sergio A Jimenez,James R Seibold,Alisa E Koch

Blood 95:294-300 PubMed10607715

1999

Viral latent membrane protein 1 (LMP-1)-induced CD99 down-regulation in B cells leads to the generation of cells with Hodgkin's and Reed-Sternberg phenotype.

Applications

Unspecified application

Species

Unspecified reactive species

S H Kim,Y K Shin,I S Lee,Y M Bae,H W Sohn,Y H Suh,H J Ree,M Rowe,S H Park
View all publications
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Product promise

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