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AB226021

Anti-CD99 antibody [EPR3096] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal CD99 antibody. Carrier free. Suitable for IHC-P, IP, Flow Cyt, WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

CD99, MIC2, MIC2X, MIC2Y, CD99 antigen, 12E7, E2 antigen, Protein MIC2, T-cell surface glycoprotein E2

7 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD99 antibody [EPR3096] - BSA and Azide free (AB226021)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD99 antibody [EPR3096] - BSA and Azide free (AB226021)

Immunohistochemical staining of paraffin-embedded Human uterus tissue using ab108297 at a dilution of 1/250.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108297).

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Flow Cytometry - Anti-CD99 antibody [EPR3096] - BSA and Azide free (AB226021)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-CD99 antibody [EPR3096] - BSA and Azide free (AB226021)

Flow cytometric analysis of Molt-4 cells using anti-CD99 RabMAb (red) (ab108297) or a rabbit IgG (negative) (green).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108297).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD99 antibody [EPR3096] - BSA and Azide free (AB226021)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD99 antibody [EPR3096] - BSA and Azide free (AB226021)

Immunohistochemical staining of paraffin-embedded Human pancreas tissue using ab108297 at a dilution of 1/250.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108297).

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-CD99 antibody [EPR3096] - BSA and Azide free (AB226021)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-CD99 antibody [EPR3096] - BSA and Azide free (AB226021)

Immunocytochemistry/Immunofluorescence analysis of Jurkat (Human T cell leukemia cell line from peripheral blood) labelling CD99 with purified ab108297 at 1/500. Cells were fixed with 4% PFA and permeabilized with 0.1% triton X-100. ab150077 Goat anti rabbit IgG (Alexa Fluor® 488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108297).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD99 antibody [EPR3096] - BSA and Azide free (AB226021)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD99 antibody [EPR3096] - BSA and Azide free (AB226021)

Immunohistochemical staining of paraffin-embedded Human tonsil tissue using ab108297 at a dilution of 1/250.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108297).

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD99 antibody [EPR3096] - BSA and Azide free (AB226021)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD99 antibody [EPR3096] - BSA and Azide free (AB226021)

Fluorescent immunohistochemical analysis of paraffin-embedded human normal pancreas tissue using ab108297. Green-CD99 red-PI

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108297).

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunoprecipitation - Anti-CD99 antibody [EPR3096] - BSA and Azide free (AB226021)
  • IP

Lab

Immunoprecipitation - Anti-CD99 antibody [EPR3096] - BSA and Azide free (AB226021)

This data was developed using ab108297, the same antibody clone in a different buffer formulation.

CD99 was immunoprecipitated from 0.35 mg HUVEC (Human umbilical vein endothelial cell) whole cell lysate 10 μg with ab108297 at 1/50 dilution (2μg). VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : HUVEC (Human umbilical vein endothelial cell) whole cell lysate 10 μg

Lane 2 : ab108297 IP in HUVEC whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab108297 in HUVEC whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

CD99 homodimer was reported in literature. (PMID : 18160845)

All lanes:

Immunoprecipitation - Anti-CD99 antibody [EPR3096] (<a href='/en-us/products/primary-antibodies/cd99-antibody-epr3096-ab108297'>ab108297</a>)

Predicted band size: 19 kDa

Observed band size: 28 kDa,32 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3096

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IP, Flow Cyt, WB, IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Alternative Products

Primary Antibodies

AB233926

Anti-CD99 antibody [HO36-1.1]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD99 antibody [HO36-1.1] (AB233926)

  • 0
  • 0
Primary Antibodies

AB212605

Anti-CD99 antibody [HO36-1.1] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD99 antibody [HO36-1.1] - BSA and Azide free (AB212605)

  • 0
  • 0
Primary Antibodies

AB130456

FITC Anti-CD99 antibody [3B2/TA8]

Flow Cytometry - FITC Anti-CD99 antibody [3B2/TA8] (AB130456)

  • 0
  • 0
Primary Antibodies

AB227738

Anti-CD99 antibody [SP119]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD99 antibody [SP119] (AB227738)

  • 0
  • 0
Primary Antibodies

AB75858

Anti-CD99 antibody [EPR3097Y]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD99 antibody [EPR3097Y] (AB75858)

  • 0
  • 0
Primary Antibodies

AB240990

Anti-CD99 antibody [SP119] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD99 antibody [SP119] - BSA and Azide free (AB240990)

  • 0
  • 0
Primary Antibodies

AB23617

Anti-CD99 antibody [DN16]

  • 0
  • 0
Primary Antibodies

AB27271

Anti-CD99 antibody

Western blot - Anti-CD99 antibody (AB27271)

  • 0
  • 0

Complementary Products

Assay Kits

AB309394

Human CD99 ELISA Kit

Sandwich ELISA - Human CD99 ELISA Kit (AB309394)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB10558

Anti-CD45 antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody (AB10558)

  • 4
  • 61
Primary Antibodies

AB955

Anti-CD68 antibody [KP1]

Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1] (AB955)

  • 4
  • 48
Primary Antibodies

AB64088

Anti-CD20 antibody [SP32]

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [SP32] (AB64088)

  • 5
  • 12
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-low-endotoxin-azide-free-ab199376'>ab199376</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Product details

ab226021 is the carrier-free version of ab108297.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD99 also known as MIC2 or HO36 is a transmembrane protein of approximately 32-35 kDa. Expressed mainly on the surface of human cells CD99 plays a role in several physiological processes. It is prominently found on T cells B cells granulocytes and some capillary endothelial cells. The protein interacts with homophilic binding partners and influences various cellular functions depending on its location and cellular context. Immunohistochemistry for CD99 (CD99 IHC) is commonly used in research and diagnostic settings to study its involvement and presence in tissues.
Biological function summary

CD99 functions as a modulator in cell adhesion and migration. It facilitates the separation of leukocytes from the endothelium during transendothelial migration impacting the immune response and inflammation. CD99 is not part of a complex itself but interacts with extracellular matrix (ECM) components to perform its role. It contributes to the regulation of cell death pathways showcasing its versatility in maintaining cellular homeostasis.

Pathways

CD99 primarily participates in the regulation of T-cell adhesion and transmigration pathways. It supports the E-selectin and integrins pathway which is important for T-cell migration to sites of inflammation. CD99 works in conjunction with proteins like ICAM-1 and VCAM-1 assisting these proteins in facilitating immune cell movement and interactions. Its involvement in these pathways highlights its significance in immune surveillance and response modulation.

CD99 shows a notable connection with Ewing sarcoma and T-cell acute lymphoblastic leukemia (T-ALL). It is highly expressed in Ewing sarcoma a malignant bone tumor and can aid in diagnostic identification alongside other markers such as HOXA9. Furthermore its expression profiles in T-ALL patients suggest a potential role in the progression or maintenance of the disease. CD99's interactions with these malignancies make it a potential target in therapeutic research exploring pathways and molecules that might modulate its expression or function.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Involved in T-cell adhesion processes (By similarity).
See full target information CD99
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

BMC anesthesiology 20:275 PubMed33126850

2020

Bupivacaine suppresses the progression of gastric cancer through regulating circ_0000376/miR-145-5p axis.

Applications

Unspecified application

Species

Unspecified reactive species

Changqiao Ju,Jia Zhou,Hui Miao,Xin Chen,Qingyu Zhang
View all publications
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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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