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Rabbit Recombinant Monoclonal CD99 antibody. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.

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Images

Immunoprecipitation - Anti-CD99 antibody [EPR3097Y] (AB75858), expandable thumbnail
  • Western blot - Anti-CD99 antibody [EPR3097Y] (AB75858), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD99 antibody [EPR3097Y] (AB75858), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD99 antibody [EPR3097Y] (AB75858), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD99 antibody [EPR3097Y] (AB75858), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWBICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Tested
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/2000
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
1/20
Notes

For unpurified use at 1/100

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

10 products for Alternative Product

Target data

Function

Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Involved in T-cell adhesion processes (By similarity).

Alternative names

CD99, MIC2, MIC2X, MIC2Y, CD99 antigen, 12E7, E2 antigen, Protein MIC2, T-cell surface glycoprotein E2

Recommended products

Rabbit Recombinant Monoclonal CD99 antibody. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR3097Y
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Stable for 12 months at -20°C

Notes

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

CD99 also known as MIC2 or HO36 is a transmembrane protein of approximately 32-35 kDa. Expressed mainly on the surface of human cells CD99 plays a role in several physiological processes. It is prominently found on T cells B cells granulocytes and some capillary endothelial cells. The protein interacts with homophilic binding partners and influences various cellular functions depending on its location and cellular context. Immunohistochemistry for CD99 (CD99 IHC) is commonly used in research and diagnostic settings to study its involvement and presence in tissues.

Biological function summary

CD99 functions as a modulator in cell adhesion and migration. It facilitates the separation of leukocytes from the endothelium during transendothelial migration impacting the immune response and inflammation. CD99 is not part of a complex itself but interacts with extracellular matrix (ECM) components to perform its role. It contributes to the regulation of cell death pathways showcasing its versatility in maintaining cellular homeostasis.

Pathways

CD99 primarily participates in the regulation of T-cell adhesion and transmigration pathways. It supports the E-selectin and integrins pathway which is important for T-cell migration to sites of inflammation. CD99 works in conjunction with proteins like ICAM-1 and VCAM-1 assisting these proteins in facilitating immune cell movement and interactions. Its involvement in these pathways highlights its significance in immune surveillance and response modulation.

Associated diseases and disorders

CD99 shows a notable connection with Ewing sarcoma and T-cell acute lymphoblastic leukemia (T-ALL). It is highly expressed in Ewing sarcoma a malignant bone tumor and can aid in diagnostic identification alongside other markers such as HOXA9. Furthermore its expression profiles in T-ALL patients suggest a potential role in the progression or maintenance of the disease. CD99's interactions with these malignancies make it a potential target in therapeutic research exploring pathways and molecules that might modulate its expression or function.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Immunoprecipitation - Anti-CD99 antibody [EPR3097Y] (ab75858), expandable thumbnail

    Immunoprecipitation - Anti-CD99 antibody [EPR3097Y] (ab75858)

    ab75858 (Purified) at 1:20 dilution (0.6 µg) immunoprecipitating CD99 in MOLT-4 whole cell lysate.
    Lane 1 (input): MOLT-4 (Human lymphoblastic leukemia T lymphoblast) whole cell lysate 10 µg
    Lane 2 (+): ab75858 & MOLT-4 whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab75858 in MOLT-4 whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75858)

    All lanes: Immunoprecipitation - Anti-CD99 antibody [EPR3097Y] (ab75858)

    Predicted band size: 19 kDa

  • Western blot - Anti-CD99 antibody [EPR3097Y] (ab75858), expandable thumbnail

    Western blot - Anti-CD99 antibody [EPR3097Y] (ab75858)

    All lanes: Western blot - Anti-CD99 antibody [EPR3097Y] (ab75858) at 1/1000 dilution

    All lanes: THP-1 (Human monocytic leukemia monocyte) whole cell lysates at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 19 kDa

    Observed band size: 32 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD99 antibody [EPR3097Y] (ab75858), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD99 antibody [EPR3097Y] (ab75858)

    ab75858 at 1/1000 dilution staining CD99 in human Ewing's sarcoma by Immunohistochemistry, Paraffin-embedded tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD99 antibody [EPR3097Y] (ab75858), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD99 antibody [EPR3097Y] (ab75858)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostatic hyperplasia tissue sections labeling CD99 with Purified ab75858 at 1:1000 dilution (0.14 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD99 antibody [EPR3097Y] (ab75858), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD99 antibody [EPR3097Y] (ab75858)

    ab75858 at 1/1000 dilution staining CD99 in human tonsil by Immunohistochemistry, Paraffin-embedded tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Flow Cytometry (Intracellular) - Anti-CD99 antibody [EPR3097Y] (ab75858), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-CD99 antibody [EPR3097Y] (ab75858)

    Intracellular Flow Cytometry analysis of Jurkat (Human acute T cell leukemia) labeling CD99with purified ab75858at 1/200 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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