AB212605

Anti-CD99 antibody [HO36-1.1] - BSA and Azide free

Name: Anti-CD99 antibody [HO36-1.1] - BSA and Azide free (ab212605) | Abcam
Brand: Abcam Limited
SKU: AB212605
Price: 905 USD
Availability: InStock

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(4 Publications)

Mouse Monoclonal CD99 antibody. Carrier free. Suitable for IHC-P and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Cell preparation containing CD99 protein.

View Alternative Names

CD99, MIC2, MIC2X, MIC2Y, CD99 antigen, 12E7, E2 antigen, Protein MIC2, T-cell surface glycoprotein E2

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD99 antibody [HO36-1.1] - BSA and Azide free (AB212605)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD99 antibody [HO36-1.1] - BSA and Azide free (AB212605)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human Ewing's sarcoma tissue labeling CD99 with ab212605 at 2 μg/ml.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD99 antibody [HO36-1.1] - BSA and Azide free (AB212605)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human testicular carcinoma tissue labeling CD99 with ab212605 at 2 μg/ml.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

HO36-1.1

Isotype

IgM

Light chain type

kappa

Carrier free

Yes

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Cell preparation containing CD99 protein. The exact immunogen used to generate this antibody is proprietary information.

P14209

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "<p>for 30 minutes at RT</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein L

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD99 also known as MIC2 or HO36 is a transmembrane protein of approximately 32-35 kDa. Expressed mainly on the surface of human cells CD99 plays a role in several physiological processes. It is prominently found on T cells B cells granulocytes and some capillary endothelial cells. The protein interacts with homophilic binding partners and influences various cellular functions depending on its location and cellular context. Immunohistochemistry for CD99 (CD99 IHC) is commonly used in research and diagnostic settings to study its involvement and presence in tissues.

Biological function summary

CD99 functions as a modulator in cell adhesion and migration. It facilitates the separation of leukocytes from the endothelium during transendothelial migration impacting the immune response and inflammation. CD99 is not part of a complex itself but interacts with extracellular matrix (ECM) components to perform its role. It contributes to the regulation of cell death pathways showcasing its versatility in maintaining cellular homeostasis.

Pathways

CD99 primarily participates in the regulation of T-cell adhesion and transmigration pathways. It supports the E-selectin and integrins pathway which is important for T-cell migration to sites of inflammation. CD99 works in conjunction with proteins like ICAM-1 and VCAM-1 assisting these proteins in facilitating immune cell movement and interactions. Its involvement in these pathways highlights its significance in immune surveillance and response modulation.

CD99 shows a notable connection with Ewing sarcoma and T-cell acute lymphoblastic leukemia (T-ALL). It is highly expressed in Ewing sarcoma a malignant bone tumor and can aid in diagnostic identification alongside other markers such as HOXA9. Furthermore its expression profiles in T-ALL patients suggest a potential role in the progression or maintenance of the disease. CD99's interactions with these malignancies make it a potential target in therapeutic research exploring pathways and molecules that might modulate its expression or function.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Involved in T-cell adhesion processes (By similarity).

See full target information CD99

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Journal of molecular biology 434:167402 PubMed34958778

2021

High-valency Anti-CD99 Antibodies Toward the Treatment of T Cell Acute Lymphoblastic Leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Larizbeth A Romero,Takamitsu Hattori,Mohamed A E Ali,Gayatri Ketavarapu,Akiko Koide,Christopher Y Park,Shohei Koide

PloS one 16:e0253170 PubMed34133426

2021

Clofarabine induces ERK/MSK/CREB activation through inhibiting CD99 on Ewing sarcoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Handan Sevim,Haydar Çelik,Levent Düşünceli,Ceyda S Ceyhan,Anna Molotkova,Kay Nakazawa,Garrett T Graham,Jeffrey R Petro,Jeffrey A Toretsky,Aykut Üren

Nature communications 12:2693 PubMed33976158

2021

An RFC4/Notch1 signaling feedback loop promotes NSCLC metastasis and stemness.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Liu,Tianyu Tao,Shihua Liu,Xia Yang,Xuwei Chen,Jiaer Liang,Ruohui Hong,Wenting Wang,Yi Yang,Xiaoyi Li,Youhong Zhang,Quanfeng Li,Shujun Liang,Haocheng Yu,Yun Wu,Xinyu Guo,Yan Lai,Xiaofan Ding,Hongyu Guan,Jueheng Wu,Xun Zhu,Jie Yuan,Jun Li,Shicheng Su,Mengfeng Li,Xiuyu Cai,Junchao Cai,Han Tian

Frontiers in oncology 11:645757 PubMed33828989

2021

NSG Mice Facilitate Characterization of Ewing Sarcoma Lung Metastasis Using the PuMA Model.

Applications

Unspecified application

Species

Unspecified reactive species

Renata Scopim-Ribeiro,Michael M Lizardo,Hai-Feng Zhang,Anne-Chloé Dhez,Chistopher S Hughes,Poul H Sorensen

View all publications

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