JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB253291

Anti-CD99 antibody [M026] - Extracellular domain

Be the first to review this product! Submit a review

|

(1 Publication)

Mouse Monoclonal CD99 antibody. Extracellular domain. Suitable for ELISA, WB, ICC/IF and reacts with Recombinant fragment - Human, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CD99.

View Alternative Names

CD99, MIC2, MIC2X, MIC2Y, CD99 antigen, 12E7, E2 antigen, Protein MIC2, T-cell surface glycoprotein E2

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-CD99 antibody [M026] - Extracellular domain (AB253291)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CD99 antibody [M026] - Extracellular domain (AB253291)

Paraformaldehyde-fixed MeWo (human malignant melanoma cell line) cells labeling CD99 using ab253291 at a 1/100 dilution in ICC/IF. Goat anti-mouse DyLight® 594 secondary.

ELISA - Anti-CD99 antibody [M026] - Extracellular domain (AB253291)
  • ELISA

Supplier Data

ELISA - Anti-CD99 antibody [M026] - Extracellular domain (AB253291)

Representative Standard Curve using mouse monoclonal anti-CD99 (ab253291) for ELISA capture of human recombinant CD99 extracellular region with His-tag. Capture was detected by using an anti-His-tag antibody followed by appropriate secondary antibody conjugated to HRP.

Western blot - Anti-CD99 antibody [M026] - Extracellular domain (AB253291)
  • WB

Supplier Data

Western blot - Anti-CD99 antibody [M026] - Extracellular domain (AB253291)

Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween-20 for 1 hour at room temperature.

All lanes:

Western blot - Anti-CD99 antibody [M026] - Extracellular domain (ab253291) at 1/1000 dilution

Lane 1:

A549 (Human lung carcinoma cell line) cell lysate

Lane 2:

A431 (Human epidermoid carcinoma cell line) cell lysate

Lane 3:

LNCaP (Human prostate cancer cell line) cell lysate

Lane 4:

MDA-MB-231 (Human cell line) cell lysate

Lane 5:

MeWo (Human malignant melanoma cell line) cell lysate

Predicted band size: 19 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

M026

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB, ELISA

applications

Immunogen

Recombinant Fragment Protein within Human CD99. The exact immunogen used to generate this antibody is proprietary information.

P14209

Specificity

Detects the short and long forms of CD99.

style
left-column

Complementary Products

Assay Kits

AB309394

Human CD99 ELISA Kit

Sandwich ELISA - Human CD99 ELISA Kit (AB309394)

  • 0
  • 0
Primary Antibodies

AB281586

Anti-CD45 antibody [RM1007]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody [RM1007] (AB281586)

  • 5
  • 3
Primary Antibodies

AB183742

Anti-TLE 1 antibody [EPR9386(2)]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-TLE 1 antibody [EPR9386(2)] (AB183742)

  • 5
  • 1
Primary Antibodies

AB124800

Anti-Myogenin antibody [EPR4789]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myogenin antibody [EPR4789] (AB124800)

  • 5
  • 3
Primary Antibodies

AB281583

Anti-CD31 antibody [RM1006]

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [RM1006] (AB281583)

  • 5
  • 5
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" }, "Recombinant fragment - Human": { "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purified from TCS.
Storage buffer
Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD99 also known as MIC2 or HO36 is a transmembrane protein of approximately 32-35 kDa. Expressed mainly on the surface of human cells CD99 plays a role in several physiological processes. It is prominently found on T cells B cells granulocytes and some capillary endothelial cells. The protein interacts with homophilic binding partners and influences various cellular functions depending on its location and cellular context. Immunohistochemistry for CD99 (CD99 IHC) is commonly used in research and diagnostic settings to study its involvement and presence in tissues.
Biological function summary

CD99 functions as a modulator in cell adhesion and migration. It facilitates the separation of leukocytes from the endothelium during transendothelial migration impacting the immune response and inflammation. CD99 is not part of a complex itself but interacts with extracellular matrix (ECM) components to perform its role. It contributes to the regulation of cell death pathways showcasing its versatility in maintaining cellular homeostasis.

Pathways

CD99 primarily participates in the regulation of T-cell adhesion and transmigration pathways. It supports the E-selectin and integrins pathway which is important for T-cell migration to sites of inflammation. CD99 works in conjunction with proteins like ICAM-1 and VCAM-1 assisting these proteins in facilitating immune cell movement and interactions. Its involvement in these pathways highlights its significance in immune surveillance and response modulation.

CD99 shows a notable connection with Ewing sarcoma and T-cell acute lymphoblastic leukemia (T-ALL). It is highly expressed in Ewing sarcoma a malignant bone tumor and can aid in diagnostic identification alongside other markers such as HOXA9. Furthermore its expression profiles in T-ALL patients suggest a potential role in the progression or maintenance of the disease. CD99's interactions with these malignancies make it a potential target in therapeutic research exploring pathways and molecules that might modulate its expression or function.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Involved in T-cell adhesion processes (By similarity).
See full target information CD99
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of neuroinflammation 18:172 PubMed34372877

2021

Increasing toll-like receptor 2 on astrocytes induced by Schwann cell-derived exosomes promotes recovery by inhibiting CSPGs deposition after spinal cord injury.

Applications

Unspecified application

Species

Unspecified reactive species

Dayu Pan,Yongjin Li,Fuhan Yang,Zenghui Lv,Shibo Zhu,Yixin Shao,Ying Huang,Guangzhi Ning,Shiqing Feng
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com