JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB227738

Anti-CD99 antibody [SP119]

Be the first to review this product! Submit a review

|

(2 Publications)

Rabbit Recombinant Monoclonal CD99 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 2 publications.

View Alternative Names

CD99, MIC2, MIC2X, MIC2Y, CD99 antigen, 12E7, E2 antigen, Protein MIC2, T-cell surface glycoprotein E2

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD99 antibody [SP119] (AB227738)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD99 antibody [SP119] (AB227738)

Formalin-fixed, paraffin-embedded human hepatocellular carcinoma tissue stained for CD99 using ab227738 at 1/200 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD99 antibody [SP119] (AB227738)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD99 antibody [SP119] (AB227738)

Formalin-fixed, paraffin-embedded human thymus tissue stained for CD99 using ab227738 at 1/200 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD99 antibody [SP119] (AB227738)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD99 antibody [SP119] (AB227738)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD99 using ab227738 at 1/200 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD99 antibody [SP119] (AB227738)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD99 antibody [SP119] (AB227738)

Formalin-fixed, paraffin-embedded human pancreas tissue stained for CD99 using ab227738 at 1/200 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD99 antibody [SP119] (AB227738)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD99 antibody [SP119] (AB227738)

Formalin-fixed, paraffin-embedded human pancreas tissue stained for CD99 using ab227738 at 1/200 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD99 antibody [SP119] (AB227738)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD99 antibody [SP119] (AB227738)

Formalin-fixed, paraffin-embedded human ovary tissue stained for CD99 using ab227738 at 1/200 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP119

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200", "IHCP-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Purification notes
Purified from TCS by protein A/G.
Storage buffer
pH: 7.6 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD99 also known as MIC2 or HO36 is a transmembrane protein of approximately 32-35 kDa. Expressed mainly on the surface of human cells CD99 plays a role in several physiological processes. It is prominently found on T cells B cells granulocytes and some capillary endothelial cells. The protein interacts with homophilic binding partners and influences various cellular functions depending on its location and cellular context. Immunohistochemistry for CD99 (CD99 IHC) is commonly used in research and diagnostic settings to study its involvement and presence in tissues.
Biological function summary

CD99 functions as a modulator in cell adhesion and migration. It facilitates the separation of leukocytes from the endothelium during transendothelial migration impacting the immune response and inflammation. CD99 is not part of a complex itself but interacts with extracellular matrix (ECM) components to perform its role. It contributes to the regulation of cell death pathways showcasing its versatility in maintaining cellular homeostasis.

Pathways

CD99 primarily participates in the regulation of T-cell adhesion and transmigration pathways. It supports the E-selectin and integrins pathway which is important for T-cell migration to sites of inflammation. CD99 works in conjunction with proteins like ICAM-1 and VCAM-1 assisting these proteins in facilitating immune cell movement and interactions. Its involvement in these pathways highlights its significance in immune surveillance and response modulation.

CD99 shows a notable connection with Ewing sarcoma and T-cell acute lymphoblastic leukemia (T-ALL). It is highly expressed in Ewing sarcoma a malignant bone tumor and can aid in diagnostic identification alongside other markers such as HOXA9. Furthermore its expression profiles in T-ALL patients suggest a potential role in the progression or maintenance of the disease. CD99's interactions with these malignancies make it a potential target in therapeutic research exploring pathways and molecules that might modulate its expression or function.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Involved in T-cell adhesion processes (By similarity).
See full target information CD99
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 23: PubMed35806460

2022

Plocabulin, a Novel Tubulin Inhibitor, Has Potent Antitumour Activity in Patient-Derived Xenograft Models of Soft Tissue Sarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yannick Wang,Agnieszka Wozniak,Jasmien Cornillie,Pablo Avilés,Maria Debiec-Rychter,Raf Sciot,Patrick Schöffski

Bioengineered 13:5126-5140 PubMed35170372

2022

Circ_0020123 enhances the cisplatin resistance in non-small cell lung cancer cells partly by sponging miR-140-3p to regulate homeobox B5 (HOXB5).

Applications

Unspecified application

Species

Unspecified reactive species

Dong Wei,Jing Zeng,Feng Rong,Yasheng Xu,Rong Wei,Can Zou
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB208372

Alexa Fluor® 488 Anti-CD99 antibody [EPR3096]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-CD99 antibody [EPR3096] (AB208372)

  • 0
  • 0
Primary Antibodies

AB75858

Anti-CD99 antibody [EPR3097Y]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD99 antibody [EPR3097Y] (AB75858)

  • 0
  • 0
Primary Antibodies

AB226021

Anti-CD99 antibody [EPR3096] - BSA and Azide free

RabMAb|Recombinant

Flow Cytometry - Anti-CD99 antibody [EPR3096] - BSA and Azide free (AB226021)

  • 0
  • 0
Primary Antibodies

AB108297

Anti-CD99 antibody [EPR3096]

RabMAb|Recombinant

Flow Cytometry - Anti-CD99 antibody [EPR3096] (AB108297)

  • 0
  • 0

Complementary Products

Assay Kits

AB309394

Human CD99 ELISA Kit

Sandwich ELISA - Human CD99 ELISA Kit (AB309394)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB10558

Anti-CD45 antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody (AB10558)

  • 4
  • 61
Primary Antibodies

AB955

Anti-CD68 antibody [KP1]

Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1] (AB955)

  • 4
  • 48
Primary Antibodies

AB64088

Anti-CD20 antibody [SP32]

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [SP32] (AB64088)

  • 5
  • 12

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com