JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB137605

Anti-CDA antibody

Be the first to review this product! Submit a review

|

(2 Publications)

Rabbit Polyclonal CDA antibody. Suitable for IHC-P and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CDA aa 1 to C-terminus.

View Alternative Names

CDD, CDA, Cytidine deaminase, Cytidine aminohydrolase

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDA antibody (AB137605)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDA antibody (AB137605)

Immunohistochemical analysis of paraffin-embedded Gastric CA N87 xenograft labelling CDA with ab137605 at 1/100 dilution.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human CDA aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P32320

style
left-column

Alternative Products

Primary Antibodies

AB78231

Anti-CDA antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDA antibody (AB78231)

  • 0
  • 0
Primary Antibodies

AB231981

Anti-CDA antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDA antibody (AB231981)

  • 0
  • 0
Primary Antibodies

AB222515

Anti-CDA antibody [EPR20525]

RabMAb|Recombinant|KO Validated|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDA antibody [EPR20525] (AB222515)

  • 0
  • 0
Primary Antibodies

AB227815

Anti-CDA antibody [EPR20525] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDA antibody [EPR20525] - BSA and Azide free (AB227815)

  • 0
  • 0

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230
Primary Antibodies

AB32577

Anti-Caveolin-1 antibody [E249] - Caveolae Marker

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-1 antibody [E249] - Caveolae Marker (AB32577)

  • 5
  • 2
Primary Antibodies

AB209538

Anti-Alpha-synuclein aggregate antibody [MJFR-14-6-4-2] - Conformation-Specific

20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Alpha-synuclein aggregate antibody [MJFR-14-6-4-2] - Conformation-Specific (AB209538)

  • 4
  • 6
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>Suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).</p>" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>Suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).</p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cytidine deaminase (CDA) also known as CDD is a zinc-dependent enzyme responsible for deaminating cytidine. The full form of CDA has a molecular mass of approximately 41 kDa. CDA is widely expressed in various tissues including liver kidney and leukocytes. It plays an important role in nucleotide metabolism by converting cytidine to uridine and 2'-deoxycytidine to 2'-deoxyuridine which impacts nucleic acid stability and synthesis. Researchers frequently use techniques such as ELISA and Western blotting to study CDA expression and activity often referencing it in contexts like "CDA Western" or "57 seconds CDA."
Biological function summary

CDA enzyme influences the pyrimidine salvage pathway by facilitating nucleotide degradation and recycling. It operates independently rather than as part of a protein complex. The activity of this enzyme ensures a balanced nucleotide pool essential for DNA replication and repair processes. This balance affects cellular proliferation and survival highlighting the importance of studying CDA protein in normal physiology and various pathological conditions.

Pathways

CDA contributes significantly to pyrimidine metabolism and its recycling pathway. It maintains a connection with ribonucleotide reductase which plays a role in the regulation of deoxyribonucleotide pools during DNA synthesis. This interplay highlights the enzyme’s involvement in DNA repair pathways ensuring proper cell division and genomic integrity. Within these pathways enzymes like cytidine triphosphate synthetase also relate to CDA by participating in nucleotide homeostasis.

Improper CDA function is linked to specific cancers including acute myeloid leukemia due to altered nucleotide metabolism leading to genomic instability. The enzyme's activity also affects the pharmacokinetics of cytidine analogues like gemcitabine used in cancer treatment. CDA overexpression might result in reduced drug efficacy thereby hindering therapy in affected patients. Connections exist between CDA and proteins involved in chemoresistance such as ribonucleotide reductase showing its significance in treatment outcomes.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

This enzyme scavenges exogenous and endogenous cytidine and 2'-deoxycytidine for UMP synthesis.
See full target information CDA
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cancers 13: PubMed34830914

2021

hENT1 Predicts Benefit from Gemcitabine in Pancreatic Cancer but Only with Low CDA mRNA.

Applications

Unspecified application

Species

Unspecified reactive species

Karen Aughton,Nils O Elander,Anthony Evans,Richard Jackson,Fiona Campbell,Eithne Costello,Christopher M Halloran,John R Mackey,Andrew G Scarfe,Juan W Valle,Ross Carter,David Cunningham,Niall C Tebbutt,David Goldstein,Jennifer Shannon,Bengt Glimelius,Thilo Hackert,Richard M Charnley,Alan Anthoney,Markus M Lerch,Julia Mayerle,Daniel H Palmer,Markus W Büchler,Paula Ghaneh,John P Neoptolemos,William Greenhalf

Disease models & mechanisms 9:1461-1471 PubMed27780828

2016

Lurbinectedin induces depletion of tumor-associated macrophages, an essential component of its in vivo synergism with gemcitabine, in pancreatic adenocarcinoma mouse models.

Applications

IHC-P

Species

Unspecified reactive species

María Virtudes Céspedes,María José Guillén,Pedro Pablo López-Casas,Francesca Sarno,Alberto Gallardo,Patricia Álamo,Carmen Cuevas,Manuel Hidalgo,Carlos María Galmarini,Paola Allavena,Pablo Avilés,Ramón Mangues
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com