Rabbit Recombinant Monoclonal CDC123 antibody. Carrier free. Suitable for Flow Cyt (Intra), WB and reacts with Human, Mouse, Rat samples.
Images
![Western blot - Anti-CDC123 antibody [EP5861] - BSA and Azide free (AB226111), expandable thumbnail](https://content.abcam.com/products/images/cdc123-antibody-ep5861-bsa-and-azide-free-ab226111--western-blot-img108818.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CDC123 antibody [EP5861] - BSA and Azide free (AB226111)")
![Flow Cytometry (Intracellular) - Anti-CDC123 antibody [EP5861] - BSA and Azide free (AB226111), expandable thumbnail](https://content.abcam.com/products/images/cdc123-antibody-ep5861-bsa-and-azide-free-ab226111--flow-cytometry-intracellular-img27170.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-CDC123 antibody [EP5861] - BSA and Azide free (AB226111)")
![OI-RD Scanning - Anti-CDC123 antibody [EP5861] - BSA and Azide free (AB226111), expandable thumbnail](https://content.abcam.com/products/images/cdc123-antibody-ep5861-bsa-and-azide-free-ab226111--oi-rd-scanning-img95385.jpg/_jcr_content/renditions/webp-475.webp "OI-RD Scanning - Anti-CDC123 antibody [EP5861] - BSA and Azide free (AB226111)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | Flow Cyt (Intra) | WB | IHC-P | |
|---|---|---|---|---|
| Human | Not recommended | Tested | Tested | Not recommended |
| Mouse | Not recommended | Expected | Tested | Not recommended |
| Rat | Not recommended | Expected | Tested | Not recommended |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human, Mouse | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Alternative names
Cell division cycle protein 123 homolog, Protein D123, HT-1080, PZ32, D123, C10orf7, CDC123
Recommended products
Rabbit Recombinant Monoclonal CDC123 antibody. Carrier free. Suitable for Flow Cyt (Intra), WB and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EP5861
- Purification technique
Affinity purification
- Dissociation constant
1.15 x 10-10 M
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab226111 is the carrier-free version of Anti-CDC123 antibody [EP5861] ab129101.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-CDC123 antibody [EP5861] - BSA and Azide free (ab226111), expandable thumbnail](https://content.abcam.com/products/images/cdc123-antibody-ep5861-bsa-and-azide-free-ab226111--western-blot-img108818.jpg "Western blot - Anti-CDC123 antibody [EP5861] - BSA and Azide free (ab226111)")
Western blot - Anti-CDC123 antibody [EP5861] - BSA and Azide free (ab226111)
This data was developed using Anti-CDC123 antibody [EP5861] ab129101, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-CDC123 antibody [EP5861] (Anti-CDC123 antibody [EP5861] ab129101) at 1/1000 dilution
Lane 1: HeLa cell lysate at 10 µg
Lane 2: 293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg
Lane 3: Caco-2 cell lysate at 10 µg
Lane 4: RAW 264.7 cell lysate at 10 µg
Lane 5: PC12 cell lysate at 10 µg
Predicted band size: 39 kDa
![Flow Cytometry (Intracellular) - Anti-CDC123 antibody [EP5861] - BSA and Azide free (ab226111), expandable thumbnail](https://content.abcam.com/products/images/cdc123-antibody-ep5861-bsa-and-azide-free-ab226111--flow-cytometry-intracellular-img27170.jpg "Flow Cytometry (Intracellular) - Anti-CDC123 antibody [EP5861] - BSA and Azide free (ab226111)")
Flow Cytometry (Intracellular) - Anti-CDC123 antibody [EP5861] - BSA and Azide free (ab226111)
This data was developed using Anti-CDC123 antibody [EP5861] ab129101, the same antibody clone in a different buffer formulation.
Anti-CDC123 antibody [EP5861] ab129101, at 1/10 dilution staining CDC123 in permeabilized HeLa cells by intracellular flow cytometry (red) or a Rabbit IgG negative control (green).
![OI-RD Scanning - Anti-CDC123 antibody [EP5861] - BSA and Azide free (ab226111), expandable thumbnail](https://content.abcam.com/products/images/cdc123-antibody-ep5861-bsa-and-azide-free-ab226111--oi-rd-scanning-img95385.jpg "OI-RD Scanning - Anti-CDC123 antibody [EP5861] - BSA and Azide free (ab226111)")
OI-RD Scanning - Anti-CDC123 antibody [EP5861] - BSA and Azide free (ab226111)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
Downloads
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