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AB10873

Anti-Cdc13 antibody [6F11/2]

3

(1 Review)

|

(8 Publications)

Mouse Monoclonal CG23 antibody. Suitable for WB and reacts with Schizosaccharomyces pombe samples. Cited in 8 publications.

View Alternative Names

SPBC582.03, cdc13, G2/mitotic-specific cyclin cdc13

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

6F11/2

Isotype

IgG2a

Carrier free

No

Applications

WB

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Schizosaccharomyces pombe": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

Cdc13 is a fission yeast (S. pombe) B-type M-phase cyclin. Cdc13 binds to Cdk1 (cdc2), and the resulting Cdk1-Cdc13 complex controls the G2/M transition of the cell cycle. 6F11/2 can be used for detecting Cdc13 and associated Cdk1 kinase activation.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cdc13 also known as Cell division cycle 13 is a telomere-binding protein and its mass is approximately 1353 amino acids. The target is expressed in yeast cells. It serves as a component of the telomeric cap structure effectively maintaining the integrity and stability of chromosome ends. Cdc13 plays a pivotal role in ensuring proper telomere length regulation and protection against degradation or fusion.
Biological function summary

Cdc13 interacts with telomerase forming part of an important complex that safeguards telomeres. This protein helps recruit telomerase to telomeres facilitating telomere maintenance during DNA replication. Through its association with other proteins like Stn1 and Ten1 Cdc13 is key in regulating telomere elongation and preventing unscheduled telomere extension.

Pathways

Cdc13 acts importantly within the telomere maintenance pathway contributing to genome stability. The protein intimately interacts within the telomerase complex comprising proteins like Est1 and Est3 ensuring the proper execution of telomerase-mediated telomere synthesis. Cdc13 influences the pathway of DNA damage response by potentially controlling telomere length and structure therefore aiding in cell cycle progression and cellular proliferation.

Cdc13's function affects genomic instability which is connected to cancer and aging-related disorders. Disruptions in Cdc13-mediated telomere maintenance can lead to telomere defects similarities to certain cancer types and premature senescence. Its role in safeguarding telomere integrity parallels the function of mammalian TRF2 protein which further emphasizes the link between telomere dysfunction Cdc13 and related conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Essential for the control of the cell cycle at the G2/M (mitosis) transition (PubMed : 2847913, PubMed : 2908246). Interacts with the cdc2 protein kinase to form MPF (PubMed : 8455610). G2/M cyclins accumulate steadily during G2 and are abruptly destroyed at mitosis (PubMed : 2534559). Involved in the reorganization of the cytoskeleton on transition from G2 to mitosis (PubMed : 2908246). Association with rum1 promotes its proteolysis during G1 (PubMed : 9303310). Also essential for initiation of meiosis II (PubMed : 7498766).
See full target information cdc13
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

FEBS letters 596:2021-2030 PubMed35770329

2022

Transcription factor Atf1-dependent degradation of the mitotic cyclin Cdc13 is regulated by multiple factors in Schizosaccharomyces pombe.

Applications

Unspecified application

Species

Unspecified reactive species

Sohini Basu,ProtitiMaiti Ghosh,Agamani Ghosal,Suchismita Datta,Geetanjali Sundaram

Autophagy reports 1:51-64 PubMed40395999

2022

Cdc13 (cyclin B) is degraded by autophagy under sulfur depletion in fission yeast.

Applications

Unspecified application

Species

Unspecified reactive species

Hokuto Ohtsuka,Yoshiko Hatta,Kana Hayashi,Takafumi Shimasaki,Yoko Otsubo,Yurika Ito,Yu Tsutsui,Nobutake Hattori,Akira Yamashita,Hiroshi Murakami,Hirofumi Aiba

Current biology : CB 30:883-892.e4 PubMed32084401

2020

The Hydrophobic Patch Directs Cyclin B to Centrosomes to Promote Global CDK Phosphorylation at Mitosis.

Applications

Unspecified application

Species

Unspecified reactive species

Souradeep Basu,Emma L Roberts,Andrew W Jones,Matthew P Swaffer,Ambrosius P Snijders,Paul Nurse

Journal of cell science 130:4028-4037 PubMed29046339

2017

A checkpoint-independent mechanism delays entry into mitosis after UV irradiation.

Applications

Unspecified application

Species

Unspecified reactive species

Christiane Rothe,Gro Elise Rødland,Silje Anda,Vilte Stonyte,Erik Boye,Sandra Lopez-Aviles,Beáta Grallert

PloS one 10:e0130000 PubMed26147350

2015

The Protein Level of Rev1, a TLS Polymerase in Fission Yeast, Is Strictly Regulated during the Cell Cycle and after DNA Damage.

Applications

Unspecified application

Species

Unspecified reactive species

Masashi Uchiyama,Junko Terunuma,Fumio Hanaoka

Eukaryotic cell 13:813-21 PubMed24728197

2014

The basic leucine zipper domain transcription factor Atf1 directly controls Cdc13 expression and regulates mitotic entry independently of Wee1 and Cdc25 in Schizosaccharomyces pombe.

Applications

Unspecified application

Species

Schizosaccharomyces pombe

Sushobhana Bandyopadhyay,Isha Dey,Megalakshmi Suresh,Geetanjali Sundaram

Cell cycle (Georgetown, Tex.) 9:3751-60 PubMed20855961

2010

Spo5 phosphorylation is essential for its own timely degradation and for successful meiosis in Schizosaccharomyces pombe.

Applications

WB

Species

Schizosaccharomyces pombe

Daisuke Okuzaki,Takashi Kasama,Aiko Hirata,Ayami Ohtaka,Reiko Kakegawa,Hiroshi Nojima

Journal of cell science 114:2627-40 PubMed11683390

2001

In vivo localisation of fission yeast cyclin-dependent kinase cdc2p and cyclin B cdc13p during mitosis and meiosis.

Applications

Unspecified application

Species

Unspecified reactive species

A Decottignies,P Zarzov,P Nurse
View all publications
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