AB183479

Anti-Cdc20 antibody

Name: Anti-Cdc20 antibody (ab183479) | Abcam
Brand: Abcam Limited
SKU: AB183479
Price: 535 USD
Availability: InStock

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(18 Publications)

Rabbit Polyclonal CDC20 antibody. Suitable for IP, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 18 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Cell division cycle protein 20 homolog aa 1-300.

View Alternative Names

Cell division cycle protein 20 homolog, p55CDC, CDC20

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-Cdc20 antibody (AB183479)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Cdc20 antibody (AB183479)

Immunofluorescent analysis of U2OS cells showing labeling of Cdc20 with ab183479 at 1/1000 dilution. Anti-Cyclin B labeling is seen in red and DAPI counterstain in blue. Lower image is merged.

Supplier Data

Western blot - Anti-Cdc20 antibody (AB183479)

All lanes:

Western blot - Anti-Cdc20 antibody (ab183479) at 1/2000 dilution

Lane 1:

U2OS cells transfected with control si RNA at 40 µg

Lane 2:

U2OS cells transfected with Cdc20 si RNA at 40 µg

Lane 3:

Rat INS1 cell lysate at 40 µg

Lane 4:

Mouse NIH 3T3 cell lysate at 40 µg

Predicted band size: 55 kDa

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Supplier Data

Immunoprecipitation - Anti-Cdc20 antibody (AB183479)

Western blot analysis of Immunoprecipitation using U2OS cells

Lane 1 : Input.

Lane 2 : control serum.

Lane 3 : ab183479.

All lanes:

Immunoprecipitation - Anti-Cdc20 antibody (ab183479)

Predicted band size: 55 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IP, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human Cell division cycle protein 20 homolog aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

Q12834

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purity

Whole antiserum

Storage buffer

Preservative: 0.05% Sodium azide

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cdc20 also known as cell division cycle 20 is an important regulatory protein in the cell cycle. It plays an essential role in the anaphase-promoting complex/cyclosome (APC/C) a large ubiquitin ligase complex important for the progression of the cell cycle. Cdc20 is expressed in various tissues acting as a cell cycle regulator during mitosis. It has a molecular weight of approximately 55 kDa. The Cdc20 protein ensures the timely degradation of key mitotic inhibitors facilitating proper chromosome segregation and mitotic exit by tagging these inhibitors for proteasomal degradation.

Biological function summary

Cdc20 acts as an activator of the APC/C complex regulating the transition from metaphase to anaphase during cell division. This transition depends on the degradation of securin and cyclins ensuring correct chromosome separation and cell cycle progression. By forming a complex with APC/C Cdc20 functions to target proteins for destruction modulating cell division dynamically. Its activity ensures that chromosomes are correctly aligned and segregated preventing errors during cell division that may lead to genomic instability.

Pathways

Cdc20 integrates itself into the spindle assembly checkpoint (SAC) and the mitotic exit network (MEN). These pathways monitor chromosome alignment and ensure that cells do not proceed to anaphase until all chromosomes are properly attached to the spindle apparatus. SAC utilizes Cdc20 to regulate its inhibitory role until chromosome alignment is confirmed after which Cdc20 activates APC/C to drive anaphase onset. Moreover proteins such as BubR1 interact with Cdc20 within the SAC to inhibit premature activation of APC/C while conditions are not optimal.

Cdc20 has significant connections with cancer and chromosomal instability syndromes. Overexpression or malfunction of Cdc20 often links with various cancers such as colorectal cancer where it drives an uncontrolled cell division rate and contributes to tumorigenesis. Additionally the improper regulation of Cdc20 can impact proteins like Mad2 disrupting the spindle checkpoint and leading to aneuploidy. This disruption often ties to chromosomal instability observed in cancerous cells highlighting its role as a potential target for therapeutic intervention.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in the metaphase/anaphase transition of cell cycle (PubMed : 32666501). Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and confers substrate specificity upon the complex (PubMed : 27030811). Is regulated by MAD2L1 : in metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates. The CDC20-APC/C complex positively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. CDC20-APC/C-induced degradation of NEUROD2 induces presynaptic differentiation. The CDC20-APC/C complex promotes proper dilation formation and radial migration by degrading CCDC41 (By similarity).

See full target information Cell division cycle protein 20 homolog

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Publications (18)

Recent publications for all applications. Explore the full list and refine your search

Cellular & molecular biology letters 30:29 PubMed40045239

2025

CDC20 protects the heart from doxorubicin-induced cardiotoxicity by modulating CCDC69 degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenyu Feng,Ningning Zhang,Liang Wang,Xumin Guan,Yunpeng Xie,Yun-Long Xia

PeerJ 11:e16306 PubMed37904848

2023

High-risk histological subtype-related FAM83A hijacked FOXM1 transcriptional regulation to promote malignant progression in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Fei,Yan Yan,Guangjun Liu,Bo Peng,Yuanyuan Liu,Qiang Chen

Theriogenology 207:19-30 PubMed37257219

2023

miR-200b/MYBL2/CDK1 suppresses proliferation and induces senescence through cell cycle arrest in ovine granulosa cells.

Applications

Species

Sheep

Pengyan Song,Xiaoyong Chen,Peiying Zhang,Ying Zhou,Rongyan Zhou

Journal of Cancer 14:1515-1530 PubMed37325046

2023

Proline-rich acidic protein 1 upregulates mitotic arrest deficient 1 to promote cisplatin-resistance of colorectal carcinoma by restraining mitotic checkpoint complex assembly.

Applications

Species

Human

Jintian Song,Yigui Chen,Hui Yu,Liang Zheng,Yi Wang,Dan Li

Neuro-oncology 25:1763-1774 PubMed37186014

2023

Mitogen-induced defective mitosis transforms neural progenitor cells.

Applications

Unspecified application

Species

Unspecified reactive species

Hiba K Omairi,Cameron J Grisdale,Mathieu Meode,Alexandra K Bohm,Sophie Black,Nancy J Adam,Cassidy P Chapman,Tatiana Maroilley,John J Kelly,Maja Tarailo-Graovac,Steven J M Jones,Michael D Blough,John Gregory Cairncross

Frontiers in oncology 12:982110 PubMed36338682

2022

Identification of hub genes and construction of prognostic nomogram for patients with Wilms tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Lie Lou,Luping Chen,Yaohao Wu,Gang Zhang,Ronglin Qiu,Jianhang Su,Zhuangjie Zhao,Zijie Lu,Minyi Liao,Xiaogeng Deng

Stem cell research & therapy 13:443 PubMed36056439

2022

Knockdown of CDC20 promotes adipogenesis of bone marrow-derived stem cells by modulating β-catenin.

Applications

Unspecified application

Species

Unspecified reactive species

Yangge Du,Yunsong Liu,Yongsheng Zhou,Ping Zhang

Journal of gastrointestinal oncology 13:1058-1072 PubMed35837165

2022

Chromobox 4 (CBX4) promotes tumor progression and stemness via activating CDC20 in gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Wen Li,Honghui Chen,Zhenggen Wang,Jingjing Liu,Xinan Lei,Wen Chen

Journal of clinical laboratory analysis 36:e24609 PubMed35838025

2022

The intercorrelation among CCT6A, CDC20, CCNB1, and PLK1 expressions and their clinical value in papillary thyroid carcinoma prognostication.

Applications

IHC

Species

Human

Wei Peng,Wenqiang Li,Xiaoyong Zhang,Weili Cen,Yanan Liu

EBioMedicine 82:104139 PubMed35810562

2022

Inhibition of DYRK1A, via histone modification, promotes cardiomyocyte cell cycle activation and cardiac repair after myocardial infarction.

Applications

Species

Unspecified reactive species

Cong Lan,Caiyu Chen,Shuang Qu,Nian Cao,Hao Luo,Cheng Yu,Na Wang,Yuanzheng Xue,Xuewei Xia,Chao Fan,Hongmei Ren,Yongjian Yang,Pedro A Jose,Zaicheng Xu,Gengze Wu,Chunyu Zeng

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