AB185814

Anti-Cdc20 antibody

Name: Anti-Cdc20 antibody (ab185814) | Abcam
Brand: Abcam Limited
SKU: AB185814
Price: 535 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal CDC20 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Cell division cycle protein 20 homolog.

View Alternative Names

Cell division cycle protein 20 homolog, p55CDC, CDC20

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-Cdc20 antibody (AB185814)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Cdc20 antibody (AB185814)

Immunocytochemistry/Immunofluorescence analysis of HeLa cells using ab185814. Blue DAPI for nuclear staining.

Supplier Data

Western blot - Anti-Cdc20 antibody (AB185814)

All lanes:

Western blot - Anti-Cdc20 antibody (ab185814) at 1/500 dilution

All lanes:

293 cell lysate

Predicted band size: 55 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human Cell division cycle protein 20 homolog. The exact immunogen used to generate this antibody is proprietary information.

Q12834

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cdc20 also known as cell division cycle 20 is an important regulatory protein in the cell cycle. It plays an essential role in the anaphase-promoting complex/cyclosome (APC/C) a large ubiquitin ligase complex important for the progression of the cell cycle. Cdc20 is expressed in various tissues acting as a cell cycle regulator during mitosis. It has a molecular weight of approximately 55 kDa. The Cdc20 protein ensures the timely degradation of key mitotic inhibitors facilitating proper chromosome segregation and mitotic exit by tagging these inhibitors for proteasomal degradation.

Biological function summary

Cdc20 acts as an activator of the APC/C complex regulating the transition from metaphase to anaphase during cell division. This transition depends on the degradation of securin and cyclins ensuring correct chromosome separation and cell cycle progression. By forming a complex with APC/C Cdc20 functions to target proteins for destruction modulating cell division dynamically. Its activity ensures that chromosomes are correctly aligned and segregated preventing errors during cell division that may lead to genomic instability.

Pathways

Cdc20 integrates itself into the spindle assembly checkpoint (SAC) and the mitotic exit network (MEN). These pathways monitor chromosome alignment and ensure that cells do not proceed to anaphase until all chromosomes are properly attached to the spindle apparatus. SAC utilizes Cdc20 to regulate its inhibitory role until chromosome alignment is confirmed after which Cdc20 activates APC/C to drive anaphase onset. Moreover proteins such as BubR1 interact with Cdc20 within the SAC to inhibit premature activation of APC/C while conditions are not optimal.

Cdc20 has significant connections with cancer and chromosomal instability syndromes. Overexpression or malfunction of Cdc20 often links with various cancers such as colorectal cancer where it drives an uncontrolled cell division rate and contributes to tumorigenesis. Additionally the improper regulation of Cdc20 can impact proteins like Mad2 disrupting the spindle checkpoint and leading to aneuploidy. This disruption often ties to chromosomal instability observed in cancerous cells highlighting its role as a potential target for therapeutic intervention.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in the metaphase/anaphase transition of cell cycle (PubMed : 32666501). Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and confers substrate specificity upon the complex (PubMed : 27030811). Is regulated by MAD2L1 : in metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates. The CDC20-APC/C complex positively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. CDC20-APC/C-induced degradation of NEUROD2 induces presynaptic differentiation. The CDC20-APC/C complex promotes proper dilation formation and radial migration by degrading CCDC41 (By similarity).

See full target information Cell division cycle protein 20 homolog

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Experimental & molecular medicine 57:1555-1566 PubMed40610751

2025

The ubiquitin E3 ligase TRIM21 suppresses type I interferon signaling via STING degradation and ameliorates systemic autoimmunity.

Applications

Unspecified application

Species

Unspecified reactive species

Da Som Kim,Youngjae Park,George C Tsokos,Mi-La Cho,Seung-Ki Kwok

American journal of cancer research 12:5552-5563 PubMed36628278

2022

Claudin 19 inhibits the malignant potential of breast cancer cells by modulating extracellular matrix-associated UBE2C/Wnt signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Jingxiu Xu,Mingjie Chen,Mingyu Hu,Hanlu Wang,Zhongqiang Zuo,Jianguo Wang,Zhiyin Xie

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