AB64877

Anti-Cdc20 antibody

Name: Anti-Cdc20 antibody (ab64877) | Abcam
Brand: Abcam Limited
SKU: AB64877
Price: 545 USD
Availability: InStock

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(5 Publications)

Rabbit Polyclonal CDC20 antibody. Suitable for WB and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human Cell division cycle protein 20 homolog.

View Alternative Names

Cell division cycle protein 20 homolog, p55CDC, CDC20

1 Images

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Western blot - Anti-Cdc20 antibody (AB64877)

All lanes:

Western blot - Anti-Cdc20 antibody (ab64877) at 1/500 dilution

Lane 1:

extract from HeLa cells, (5-30µg total protein).

Lane 2:

extract from Jurkat cells, (5-30µg total protein).

Lane 3:

extract from Jurkat cells, (5-30µg total protein) and 5-10µg of the immunising peptide.

Predicted band size: 55 kDa

Observed band size: 26 kDa,34 kDa,55 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human Cell division cycle protein 20 homolog. The exact immunogen used to generate this antibody is proprietary information.

Q12834

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cdc20 also known as cell division cycle 20 is an important regulatory protein in the cell cycle. It plays an essential role in the anaphase-promoting complex/cyclosome (APC/C) a large ubiquitin ligase complex important for the progression of the cell cycle. Cdc20 is expressed in various tissues acting as a cell cycle regulator during mitosis. It has a molecular weight of approximately 55 kDa. The Cdc20 protein ensures the timely degradation of key mitotic inhibitors facilitating proper chromosome segregation and mitotic exit by tagging these inhibitors for proteasomal degradation.

Biological function summary

Cdc20 acts as an activator of the APC/C complex regulating the transition from metaphase to anaphase during cell division. This transition depends on the degradation of securin and cyclins ensuring correct chromosome separation and cell cycle progression. By forming a complex with APC/C Cdc20 functions to target proteins for destruction modulating cell division dynamically. Its activity ensures that chromosomes are correctly aligned and segregated preventing errors during cell division that may lead to genomic instability.

Pathways

Cdc20 integrates itself into the spindle assembly checkpoint (SAC) and the mitotic exit network (MEN). These pathways monitor chromosome alignment and ensure that cells do not proceed to anaphase until all chromosomes are properly attached to the spindle apparatus. SAC utilizes Cdc20 to regulate its inhibitory role until chromosome alignment is confirmed after which Cdc20 activates APC/C to drive anaphase onset. Moreover proteins such as BubR1 interact with Cdc20 within the SAC to inhibit premature activation of APC/C while conditions are not optimal.

Cdc20 has significant connections with cancer and chromosomal instability syndromes. Overexpression or malfunction of Cdc20 often links with various cancers such as colorectal cancer where it drives an uncontrolled cell division rate and contributes to tumorigenesis. Additionally the improper regulation of Cdc20 can impact proteins like Mad2 disrupting the spindle checkpoint and leading to aneuploidy. This disruption often ties to chromosomal instability observed in cancerous cells highlighting its role as a potential target for therapeutic intervention.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Involved in the metaphase/anaphase transition of cell cycle (PubMed : 32666501). Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and confers substrate specificity upon the complex (PubMed : 27030811). Is regulated by MAD2L1 : in metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates. The CDC20-APC/C complex positively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. CDC20-APC/C-induced degradation of NEUROD2 induces presynaptic differentiation. The CDC20-APC/C complex promotes proper dilation formation and radial migration by degrading CCDC41 (By similarity).

See full target information Cell division cycle protein 20 homolog

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Experimental & molecular medicine 50:46 PubMed29700288

2018

ATP depletion during mitotic arrest induces mitotic slippage and APC/C-dependent cyclin B1 degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Yun Yeon Park,Ju-Hyun Ahn,Min-Guk Cho,Jae-Ho Lee

Cell cycle (Georgetown, Tex.) 14:1459-70 PubMed25789401

2015

Stranglehold on the spindle assembly checkpoint: the human papillomavirus E2 protein provokes BUBR1-dependent aneuploidy.

Applications

Species

Human

Chye Ling Tan,Sébastien Teissier,Jayantha Gunaratne,Ling Shih Quek,Sophie Bellanger

The Journal of cell biology 206:843-53 PubMed25246615

2014

Mastl is required for timely activation of APC/C in meiosis I and Cdk1 reactivation in meiosis II.

Applications

Unspecified application

Species

Mouse

Deepak Adhikari,M Kasim Diril,Kiran Busayavalasa,Sanjiv Risal,Shoma Nakagawa,Rebecca Lindkvist,Yan Shen,Vincenzo Coppola,Lino Tessarollo,Nobuaki R Kudo,Philipp Kaldis,Kui Liu

Nature structural & molecular biology 19:1116-23 PubMed23007861

2012

APC15 mediates CDC20 autoubiquitylation by APC/C(MCC) and disassembly of the mitotic checkpoint complex.

Applications

Species

Human

Kristina Uzunova,Billy T Dye,Hannelore Schutz,Rene Ladurner,Georg Petzold,Yusuke Toyoda,Marc A Jarvis,Nicholas G Brown,Ina Poser,Maria Novatchkova,Karl Mechtler,Anthony A Hyman,Holger Stark,Brenda A Schulman,Jan-Michael Peters

Cell 141:81-93 PubMed20371347

2010

Persistent telomere damage induces bypass of mitosis and tetraploidy.

Applications

Unspecified application

Species

Unspecified reactive species

Teresa Davoli,Eros Lazzerini Denchi,Titia de Lange

View all publications

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