JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB215908

Anti-Cdc20 antibody [CDC20/1102]

Be the first to review this product! Submit a review

|

(7 Publications)

Mouse Monoclonal CDC20 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human Cell division cycle protein 20 homolog.

View Alternative Names

Cell division cycle protein 20 homolog, p55CDC, CDC20

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc20 antibody [CDC20/1102] (AB215908)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc20 antibody [CDC20/1102] (AB215908)

Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human tonsil tissue labeling Cdc20 with ab215908 at 4 μg/ml dilution.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CDC20/1102

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human Cell division cycle protein 20 homolog.

Q12834

style
left-column

Alternative Products

Primary Antibodies

AB190711

Anti-Cdc20 antibody [AR12]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc20 antibody [AR12] (AB190711)

  • 0
  • 0
Primary Antibodies

AB64877

Anti-Cdc20 antibody

Western blot - Anti-Cdc20 antibody (AB64877)

  • 0
  • 0
Primary Antibodies

AB18217

Anti-Cdc20 antibody [BA8]

Flow Cytometry - Anti-Cdc20 antibody [BA8] (AB18217)

  • 0
  • 0
Primary Antibodies

AB185814

Anti-Cdc20 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Cdc20 antibody (AB185814)

  • 0
  • 0
Primary Antibodies

AB183479

Anti-Cdc20 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Cdc20 antibody (AB183479)

  • 0
  • 0
Primary Antibodies

AB86104

Anti-Cdc20 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc20 antibody (AB86104)

  • 0
  • 0

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
Primary Antibodies

AB133327

Anti-CDK1 antibody [EPR165]

20ul selling size|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-CDK1 antibody [EPR165] (AB133327)

  • 5
  • 4
Primary Antibodies

AB32042

Anti-Cleaved Caspase-3 antibody [E83-77]

20ul selling size|RabMAb|Recombinant|KO Validated

Western blot - Anti-Cleaved Caspase-3 antibody [E83-77] (AB32042)

  • 4
  • 28
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2-4 µg/mL", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Purification notes
ab215908 is purified from Bioreactor Concentrate by Protein A/G.
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cdc20 also known as cell division cycle 20 is an important regulatory protein in the cell cycle. It plays an essential role in the anaphase-promoting complex/cyclosome (APC/C) a large ubiquitin ligase complex important for the progression of the cell cycle. Cdc20 is expressed in various tissues acting as a cell cycle regulator during mitosis. It has a molecular weight of approximately 55 kDa. The Cdc20 protein ensures the timely degradation of key mitotic inhibitors facilitating proper chromosome segregation and mitotic exit by tagging these inhibitors for proteasomal degradation.
Biological function summary

Cdc20 acts as an activator of the APC/C complex regulating the transition from metaphase to anaphase during cell division. This transition depends on the degradation of securin and cyclins ensuring correct chromosome separation and cell cycle progression. By forming a complex with APC/C Cdc20 functions to target proteins for destruction modulating cell division dynamically. Its activity ensures that chromosomes are correctly aligned and segregated preventing errors during cell division that may lead to genomic instability.

Pathways

Cdc20 integrates itself into the spindle assembly checkpoint (SAC) and the mitotic exit network (MEN). These pathways monitor chromosome alignment and ensure that cells do not proceed to anaphase until all chromosomes are properly attached to the spindle apparatus. SAC utilizes Cdc20 to regulate its inhibitory role until chromosome alignment is confirmed after which Cdc20 activates APC/C to drive anaphase onset. Moreover proteins such as BubR1 interact with Cdc20 within the SAC to inhibit premature activation of APC/C while conditions are not optimal.

Cdc20 has significant connections with cancer and chromosomal instability syndromes. Overexpression or malfunction of Cdc20 often links with various cancers such as colorectal cancer where it drives an uncontrolled cell division rate and contributes to tumorigenesis. Additionally the improper regulation of Cdc20 can impact proteins like Mad2 disrupting the spindle checkpoint and leading to aneuploidy. This disruption often ties to chromosomal instability observed in cancerous cells highlighting its role as a potential target for therapeutic intervention.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Substrate-specific adapter of the anaphase promoting complex/cyclosome (APC/C) complex that confers substrate specificity by binding to substrates and targeting them to the APC/C complex for ubiquitination and degradation (PubMed : 9734353, PubMed : 27030811, PubMed : 29343641). Recognizes and binds the destruction box (D box) on protein substrates (PubMed : 29343641). Involved in the metaphase/anaphase transition of cell cycle (PubMed : 32666501). Is regulated by MAD2L1 : in metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates (PubMed : 9811605, PubMed : 9637688). The CDC20-APC/C complex positively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons (By similarity). CDC20-APC/C-induced degradation of NEUROD2 induces presynaptic differentiation (By similarity). The CDC20-APC/C complex promotes proper dilation formation and radial migration by degrading CCDC41 (By similarity).
See full target information Cell division cycle protein 20 homolog
style
left-column

Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Osteoarthritis and cartilage open 7:100649 PubMed40735537

2025

Comorbid hypertension and osteoarthritis exacerbates joint remodeling and gait compensations in female rats with milder effects observed in males.

Applications

Unspecified application

Species

Unspecified reactive species

Carlos J Cruz,Folly M Patterson,Janak Gaire,Julianna Gonzalez,Jacob L Griffith,Angela Philistin,Kyle D Allen

Cancer medicine 13:e70284 PubMed39540710

2024

Predictive Prognostic Model for Hepatocellular Carcinoma Based on Seven Genes Participating in Arachidonic Acid Metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Xinyu Gu,Jing Wang,Jun Guan,Guojun Li,Xiao Ma,Yanli Ren,Shanshan Wu,Chao Chen,Haihong Zhu

Molecular medicine reports 26: PubMed35616161

2022

REC8 inhibits proliferation, migration and invasion of breast cancer cells by targeting CDC20.

Applications

Unspecified application

Species

Unspecified reactive species

Shaodan He,Danping Liu,Zhuanhong Chen

Digestive surgery 39:24-31 PubMed34788753

2021

Identification and Validation of Hub Genes Predicting Prognosis of Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ziyan Chen,Haitao Yu,Lijun Wu,Sina Zhang,Zhihui Lin,Tuo Deng,Bangjie He,Zhengping Yu,Gang Chen,Fang Wu

Molecular medicine reports 24: PubMed33907851

2021

CDC20 promotes the progression of hepatocellular carcinoma by regulating epithelial‑mesenchymal transition.

Applications

Unspecified application

Species

Unspecified reactive species

Gang Yang,Guan Wang,Yongfu Xiong,Ji Sun,Weinan Li,Tao Tang,Jingdong Li

Cancer letters 500:172-181 PubMed33290869

2020

Cdc20 induces the radioresistance of bladder cancer cells by targeting FoxO1 degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Lixia Wang,Chuanlai Yang,Man Chu,Zhi-Wei Wang,Boxin Xue

International journal of clinical oncology 24:1479-1489 PubMed31278532

2019

CDC20 and its downstream genes: potential prognosis factors of osteosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Man-Si Wu,Qing-Yu Ma,Dong-Dong Liu,Xiao-Juan Li,Li-Juan Deng,Nan Li,Jingnan Shen,Zhiqiang Zhao,Jia-Xu Chen
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com