Rabbit Recombinant Monoclonal CDC23 antibody. Carrier free. Suitable for ICC/IF, IP, WB and reacts with Human samples.
Images
![Western blot - Anti-Cdc23 antibody [EPR11827] - BSA and Azide free (AB249936), expandable thumbnail](https://content.abcam.com/products/images/cdc23-antibody-epr11827-bsa-and-azide-free-ab249936--western-blot-img116541.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Cdc23 antibody [EPR11827] - BSA and Azide free (AB249936)")
![Immunoprecipitation - Anti-Cdc23 antibody [EPR11827] - BSA and Azide free (AB249936), expandable thumbnail](https://content.abcam.com/products/images/cdc23-antibody-epr11827-bsa-and-azide-free-ab249936--immunoprecipitation-img131610.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Cdc23 antibody [EPR11827] - BSA and Azide free (AB249936)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | Flow Cyt | WB | IHC-P | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Tested | Not recommended |
| Rat | Predicted | Predicted | Not recommended | Predicted | Not recommended |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info - | Notes - |
Target data
Function
Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle (PubMed:18485873). The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (PubMed:18485873). The APC/C complex catalyzes assembly of branched 'Lys-11'-/'Lys-48'-linked branched ubiquitin chains on target proteins (PubMed:29033132).
Alternative names
ANAPC8, CDC23, Cell division cycle protein 23 homolog, Anaphase-promoting complex subunit 8, Cyclosome subunit 8, APC8
Recommended products
Rabbit Recombinant Monoclonal CDC23 antibody. Carrier free. Suitable for ICC/IF, IP, WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR11827
- Purification technique
- Affinity purification
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab249936 is the carrier-free version of Anti-Cdc23 antibody [EPR11827] ab177148.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Cdc23 also known as ANAPC8 is an essential component of the anaphase-promoting complex/cyclosome (APC/C). This protein has a molecular mass of approximately 64 kDa and plays an important role in controlling the cell cycle by functioning as an E3 ubiquitin ligase. Cdc23 tags target proteins for degradation through the ubiquitin-proteasome system ensuring proper cell cycle progression especially during the transition from metaphase to anaphase. It is widely expressed in proliferating cells reflecting its significant role in cell division processes.
- Biological function summary
Cdc23 contributes to the regulation of cell cycle progression. It is a part of the larger APC/C complex which oversees the degradation of cell cycle regulators such as securin and cyclins. The function of Cdc23 is key in the timely progression from metaphase to anaphase guaranteeing that chromosomes segregate accurately. This regulation protects genomic integrity and is necessary for normal cell proliferation and growth.
- Pathways
Cdc23 participates actively in the regulation of the cell cycle and the ubiquitin-proteasome pathway. It works closely with other components of the APC/C complex to modulate the activity of cyclin-dependent kinases (CDKs). This modulation ensures proper turnover of critical proteins coordinating cell cycle events. Cdc20 and Cdh1 are two other regulatory proteins that interact with Cdc23 within these pathways providing temporal control to the cell division process.
- Associated diseases and disorders
Cdc23 is connected to cancer and some neurodegenerative disorders. Aberrations in its function or expression levels can lead to uncontrolled cell growth commonly associated with different cancers. The misregulation of the APC/C complex involving Cdc23 can also impact neuronal cells linking its role to neurodegenerative diseases like Alzheimer's. The interactions with other APC/C complex proteins such as Cdc27 further illustrate the critical roles of Cdc23 in maintaining normal cellular and physiological functions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-Cdc23 antibody [EPR11827] - BSA and Azide free (ab249936), expandable thumbnail](https://content.abcam.com/products/images/cdc23-antibody-epr11827-bsa-and-azide-free-ab249936--western-blot-img116541.jpg "Western blot - Anti-Cdc23 antibody [EPR11827] - BSA and Azide free (ab249936)")
Western blot - Anti-Cdc23 antibody [EPR11827] - BSA and Azide free (ab249936)
This data was developed using Anti-Cdc23 antibody [EPR11827] ab177148, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-Cdc23 antibody [EPR11827] (Anti-Cdc23 antibody [EPR11827] ab177148) at 1/10000 dilution
Lane 1: HepG2 cell lysate at 10 µg
Lane 2: HeLa cell lysate at 10 µg
Lane 3: K562 cell lysate at 10 µg
Lane 4: Jurkat cell lysate at 10 µg
SecondaryAll lanes: Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 68 kDa
![Immunoprecipitation - Anti-Cdc23 antibody [EPR11827] - BSA and Azide free (ab249936), expandable thumbnail](https://content.abcam.com/products/images/cdc23-antibody-epr11827-bsa-and-azide-free-ab249936--immunoprecipitation-img131610.jpg "Immunoprecipitation - Anti-Cdc23 antibody [EPR11827] - BSA and Azide free (ab249936)")
Immunoprecipitation - Anti-Cdc23 antibody [EPR11827] - BSA and Azide free (ab249936)
This data was developed using Anti-Cdc23 antibody [EPR11827] ab177148, the same antibody clone in a different buffer formulation.Western blot analysis on immunoprecipitation pellet from (Lane 1) K562 cells lysate or (Lane 2) 1X PBS (negative control) using Anti-Cdc23 antibody [EPR11827] ab177148 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
All lanes: Immunoprecipitation - Anti-Cdc23 antibody [EPR11827] (Anti-Cdc23 antibody [EPR11827] ab177148)
Predicted band size: 68 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-Cdc23 antibody [EPR11827] - BSA and Azide free (ab249936), expandable thumbnail](https://content.abcam.com/products/images/cdc23-antibody-epr11827-bsa-and-azide-free-ab249936--immunocytochemistry-immunofluorescence-img132594.gif "Immunocytochemistry/ Immunofluorescence - Anti-Cdc23 antibody [EPR11827] - BSA and Azide free (ab249936)")
Immunocytochemistry/ Immunofluorescence - Anti-Cdc23 antibody [EPR11827] - BSA and Azide free (ab249936)
This data was developed using Anti-Cdc23 antibody [EPR11827] ab177148, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HeLa cells labeling Cdc23 with Anti-Cdc23 antibody [EPR11827] ab177148 at 1/50 dilution.
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