AB182003

Anti-Cdc23 antibody [EPR11828]

RabMAb
Recombinant
What is this?

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(3 Publications)

Rabbit Recombinant Monoclonal CDC23 antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 3 publications.

View Alternative Names

ANAPC8, CDC23, Cell division cycle protein 23 homolog, Anaphase-promoting complex subunit 8, Cyclosome subunit 8, APC8

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-Cdc23 antibody [EPR11828] (AB182003)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Cdc23 antibody [EPR11828] (AB182003)

Immunofluorescent analysis of acetone-fixed HeLa cells labeling Cdc23 with ab182003 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary at 1/200 dilution (Left panel). Counter stained with Dapi (Right panel).

Supplier Data

Western blot - Anti-Cdc23 antibody [EPR11828] (AB182003)

All lanes:

Western blot - Anti-Cdc23 antibody [EPR11828] (ab182003) at 1/10000 dilution

Lane 1:

HepG2 cell lysate at 20 µg

Lane 2:

HeLa cell lysate at 20 µg

Lane 3:

K562 cell lysate at 20 µg

Lane 4:

Jurkat cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 68 kDa

Observed band size: 60 kDa

false

Carrier free

Anti-Cdc23 antibody [EPR11828] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11828

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cdc23 also known as ANAPC8 is an essential component of the anaphase-promoting complex/cyclosome (APC/C). This protein has a molecular mass of approximately 64 kDa and plays an important role in controlling the cell cycle by functioning as an E3 ubiquitin ligase. Cdc23 tags target proteins for degradation through the ubiquitin-proteasome system ensuring proper cell cycle progression especially during the transition from metaphase to anaphase. It is widely expressed in proliferating cells reflecting its significant role in cell division processes.

Biological function summary

Cdc23 contributes to the regulation of cell cycle progression. It is a part of the larger APC/C complex which oversees the degradation of cell cycle regulators such as securin and cyclins. The function of Cdc23 is key in the timely progression from metaphase to anaphase guaranteeing that chromosomes segregate accurately. This regulation protects genomic integrity and is necessary for normal cell proliferation and growth.

Pathways

Cdc23 participates actively in the regulation of the cell cycle and the ubiquitin-proteasome pathway. It works closely with other components of the APC/C complex to modulate the activity of cyclin-dependent kinases (CDKs). This modulation ensures proper turnover of critical proteins coordinating cell cycle events. Cdc20 and Cdh1 are two other regulatory proteins that interact with Cdc23 within these pathways providing temporal control to the cell division process.

Cdc23 is connected to cancer and some neurodegenerative disorders. Aberrations in its function or expression levels can lead to uncontrolled cell growth commonly associated with different cancers. The misregulation of the APC/C complex involving Cdc23 can also impact neuronal cells linking its role to neurodegenerative diseases like Alzheimer's. The interactions with other APC/C complex proteins such as Cdc27 further illustrate the critical roles of Cdc23 in maintaining normal cellular and physiological functions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle (PubMed : 18485873). The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins : it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (PubMed : 18485873). The APC/C complex catalyzes assembly of branched 'Lys-11'-/'Lys-48'-linked branched ubiquitin chains on target proteins (PubMed : 29033132).

See full target information CDC23

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of cell science 135: PubMed34878135

2022

The APC/C targets the Cep152-Cep63 complex at the centrosome to regulate mitotic spindle assembly.

Applications

Unspecified application

Species

Unspecified reactive species

Thomas Tischer,Jing Yang,David Barford

Cell reports 34:108929 PubMed33789095

2021

Molecular mechanisms of APC/C release from spindle assembly checkpoint inhibition by APC/C SUMOylation.

Applications

Unspecified application

Species

Unspecified reactive species

Stanislau Yatskevich,Jessie S Kroonen,Claudio Alfieri,Thomas Tischer,Anna C Howes,Linda Clijsters,Jing Yang,Ziguo Zhang,Kaige Yan,Alfred C O Vertegaal,David Barford

BMC cancer 14:538 PubMed25064703

2014

Expression of miR-34c induces G2/M cell cycle arrest in breast cancer cells.

Applications

Species

Human

Chandrani Achari,Sofia Winslow,Yvonne Ceder,Christer Larsson

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cdc23 antibody [EPR11828]

Immunocytochemistry/ Immunofluorescence - Anti-Cdc23 antibody [EPR11828] (AB182003)

Western blot - Anti-Cdc23 antibody [EPR11828] (AB182003)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise