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AB250568

Anti-Cdc23 antibody [EPR11828] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal CDC23 antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

ANAPC8, CDC23, Cell division cycle protein 23 homolog, Anaphase-promoting complex subunit 8, Cyclosome subunit 8, APC8

2 Images
Western blot - Anti-Cdc23 antibody [EPR11828] - BSA and Azide free (AB250568)
  • WB

Supplier Data

Western blot - Anti-Cdc23 antibody [EPR11828] - BSA and Azide free (AB250568)

This data was developed using ab182003, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Cdc23 antibody [EPR11828] (<a href='/en-us/products/primary-antibodies/cdc23-antibody-epr11828-ab182003'>ab182003</a>) at 1/10000 dilution

Lane 1:

HepG2 cell lysate at 20 µg

Lane 2:

HeLa cell lysate at 20 µg

Lane 3:

K562 cell lysate at 20 µg

Lane 4:

Jurkat cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 68 kDa

Observed band size: 60 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-Cdc23 antibody [EPR11828] - BSA and Azide free (AB250568)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Cdc23 antibody [EPR11828] - BSA and Azide free (AB250568)

This data was developed using ab182003, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of acetone-fixed HeLa cells labeling Cdc23 with ab182003 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary at 1/200 dilution (Left panel). Counter stained with Dapi (Right panel).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11828

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

ab250568 is the carrier-free version of ab182003.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cdc23 also known as ANAPC8 is an essential component of the anaphase-promoting complex/cyclosome (APC/C). This protein has a molecular mass of approximately 64 kDa and plays an important role in controlling the cell cycle by functioning as an E3 ubiquitin ligase. Cdc23 tags target proteins for degradation through the ubiquitin-proteasome system ensuring proper cell cycle progression especially during the transition from metaphase to anaphase. It is widely expressed in proliferating cells reflecting its significant role in cell division processes.
Biological function summary

Cdc23 contributes to the regulation of cell cycle progression. It is a part of the larger APC/C complex which oversees the degradation of cell cycle regulators such as securin and cyclins. The function of Cdc23 is key in the timely progression from metaphase to anaphase guaranteeing that chromosomes segregate accurately. This regulation protects genomic integrity and is necessary for normal cell proliferation and growth.

Pathways

Cdc23 participates actively in the regulation of the cell cycle and the ubiquitin-proteasome pathway. It works closely with other components of the APC/C complex to modulate the activity of cyclin-dependent kinases (CDKs). This modulation ensures proper turnover of critical proteins coordinating cell cycle events. Cdc20 and Cdh1 are two other regulatory proteins that interact with Cdc23 within these pathways providing temporal control to the cell division process.

Cdc23 is connected to cancer and some neurodegenerative disorders. Aberrations in its function or expression levels can lead to uncontrolled cell growth commonly associated with different cancers. The misregulation of the APC/C complex involving Cdc23 can also impact neuronal cells linking its role to neurodegenerative diseases like Alzheimer's. The interactions with other APC/C complex proteins such as Cdc27 further illustrate the critical roles of Cdc23 in maintaining normal cellular and physiological functions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle (PubMed : 18485873). The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins : it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (PubMed : 18485873). The APC/C complex catalyzes assembly of branched 'Lys-11'-/'Lys-48'-linked branched ubiquitin chains on target proteins (PubMed : 29033132).
See full target information CDC23
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 26:458 PubMed37736555

2023

S100A6 is a potential diagnostic and prognostic biomarker for human glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Hong,Hui Zhang,Yufei Xiao,Lingwei Shen,Yun Qian
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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