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AB272920

Anti-CDC25 antibody

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Rabbit Polyclonal CDC25 antibody. Suitable for IHC-P, WB and reacts with Mouse, Rat samples. Immunogen corresponding to Synthetic Peptide within Rat Rasgrf1.

View Alternative Names

Cdc25, Grf1, Rasgrf1, Ras-specific guanine nucleotide-releasing factor 1, Ras-GRF1, CDC25Mm, Guanine nucleotide-releasing protein, Ras-specific nucleotide exchange factor CDC25, GNRP

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC25 antibody (AB272920)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC25 antibody (AB272920)

Immunohistochemical analysis of paraffin-embedded mouse brain tissue stained for RASGRF1 protein at cytoplasm with ab272920 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC25 antibody (AB272920)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC25 antibody (AB272920)

Immunohistochemical analysis of paraffin-embedded rat brain tissue stained for RASGRF1 protein at cytoplasm with ab272920 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min.

Western blot - Anti-CDC25 antibody (AB272920)
  • WB

Supplier Data

Western blot - Anti-CDC25 antibody (AB272920)

5% SDS-PAGE

All lanes:

Western blot - Anti-CDC25 antibody (ab272920) at 1/1000 dilution

Lane 1:

Mouse brain tissue extract at 30 µg

Lane 2:

Rat brain tissue extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 145 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Rat Rasgrf1. The exact immunogen used to generate this antibody is proprietary information.

P28818

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Promotes the exchange of Ras-bound GDP by GTP.
See full target information Rasgrf1
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Product promise

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