AB111830

Anti-CDC25 antibody [EPR1790(2)]

RabMAb
Recombinant
What is this?

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(5 Publications)

Rabbit Recombinant Monoclonal CDC25 antibody. Suitable for WB and reacts with Human samples. Cited in 5 publications.

View Alternative Names

CDC25, GNRP, GRF1, RASGRF1, Ras-specific guanine nucleotide-releasing factor 1, Ras-GRF1, Guanine nucleotide-releasing protein, Ras-specific nucleotide exchange factor CDC25

1 Images

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Western blot - Anti-CDC25 antibody [EPR1790(2)] (AB111830)

All lanes:

Western blot - Anti-CDC25 antibody [EPR1790(2)] (ab111830) at 1/1000 dilution

Lane 1:

Human hippocampus lysate at 10 µg

Lane 2:

Human hypothalamus lysate at 10 µg

Lane 3:

Human midbrain lysate at 10 µg

Predicted band size: 145 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR1790(2)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody detects the band of interest in tissue lysates, but it did not detect the protein in cell lysate.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Species reactivity
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Promotes the exchange of Ras-bound GDP by GTP.

See full target information RASGRF1

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

NPJ precision oncology 9:224 PubMed40615519

2025

Genetic and oncogenic features of RASGRF fusions.

Applications

Unspecified application

Species

Unspecified reactive species

Sreya Das,Daniel S Lenchner,Ellen Jaeger,Lisa Hunihan,Dana F DeSantis,Stamatina Fragkogianni,Karyn Ronski,Frederick H Wilson

Open medicine (Warsaw, Poland) 18:20230654 PubMed37197357

2023

Effect of miR-499-5p/SOX6 axis on atrial fibrosis in rats with atrial fibrillation.

Applications

Unspecified application

Species

Unspecified reactive species

Xinyuan Han,Shunda Wang,Zhijun Yong,Xueting Zhang,Xuanqi Wang,Penghua You

Clinical cancer research : an official journal of the American Association for Cancer Research 28:3091-3103 PubMed35247929

2022

RASGRF1 Fusions Activate Oncogenic RAS Signaling and Confer Sensitivity to MEK Inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Lisa Hunihan,Dejian Zhao,Heather Lazowski,Man Li,Yuping Qian,Laura Abriola,Yulia V Surovtseva,Viswanathan Muthusamy,Lynn T Tanoue,Bonnie E Gould Rothberg,Kurt A Schalper,Roy S Herbst,Frederick H Wilson

Oncology letters 20:1360-1368 PubMed32724378

2020

ALOX12B promotes carcinogenesis in cervical cancer by regulating the PI3K/ERK1 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Jiang,Bing Zhou,Yuan Meng Li,Qui Ying Yang,Kai Jia Tu,Long Yu Li

International journal of molecular sciences 18: PubMed28696354

2017

A Case of AML Characterized by a Novel t(4;15)(q31;q22) Translocation That Confers a Growth-Stimulatory Response to Retinoid-Based Therapy.

Applications

Species

Human

Justin M Watts,Aymee Perez,Lutecia Pereira,Yao-Shan Fan,Geoffrey Brown,Francisco Vega,Kevin Petrie,Ronan T Swords,Arthur Zelent

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CDC25 antibody [EPR1790(2)]

Western blot - Anti-CDC25 antibody [EPR1790(2)] (AB111830)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (5)

Product promise