AB182666

Anti-Cdc25A (phospho S124) antibody

Name: Anti-Cdc25A (phospho S124) antibody (ab182666) | Abcam
Brand: Abcam Limited
SKU: AB182666
Price: 535 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal Cdc25A phospho S124 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human CDC25A pS124 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

M-phase inducer phosphatase 1, Dual specificity phosphatase Cdc25A, CDC25A

1 Images

Supplier Data

Western blot - Anti-Cdc25A (phospho S124) antibody (AB182666)

All lanes:

Western blot - Anti-Cdc25A (phospho S124) antibody (ab182666) at 1/500 dilution

All lanes:

UV treated 293 cell extract

Predicted band size: 59 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human CDC25A pS124 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P30304

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p>Incubate membrane with diluted antibody in 2.5% nonfat milk, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab182666 was purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cdc25A also known as Cell Division Cycle 25A acts as a phosphatase that removes phosphate groups from cyclin-dependent kinases (CDKs). This process mainly activates CDKs which are important for the cell cycle progression. Cdc25A has a molecular mass of approximately 65 kDa. The protein expresses in various cell types but finds higher levels in proliferative tissues. Additionally it localizes primarily in the nucleus where it executes its function during specific cell cycle phases.

Biological function summary

Cdc25A plays a big role in controlling transitions between cell cycle phases particularly the G1 to S phase. It participates in a CDK complex which includes partners like Cyclin E and Cyclin A driving progression through cell cycle checkpoints. During DNA replication Cdc25A's activity ensures that cells duplicate accurately maintaining genome integrity and balance in cell proliferation.

Pathways

Cdc25A functions within the cell cycle regulation and DNA damage repair pathways. It interacts importantly with proteins like Checkpoint Kinase 1 and 2 (Chk1/Chk2) involved in responding to DNA damage. During adverse conditions Chk1/Chk2 phosphorylate Cdc25A leading to its degradation and preventing further cycle progression. This mechanism helps maintain genomic stability by halting the cycle until DNA repair occurs.

Cdc25A holds connections to cancer and neurodegenerative conditions. Its overexpression often links to cancers as uncontrolled activity allows for unscheduled cell proliferation. Similarly dysregulation of Cdc25A relates to proteins like p53 which suppress tumor growth. In neurodegenerative disorders Cdc25A's misregulation can also increase the potential for neuronal cell cycle re-entry contributing to neuronal death. Understanding these relationships is essential for developing therapeutic targets.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression (PubMed : 12676925, PubMed : 14559997, PubMed : 1836978, PubMed : 20360007). Directly dephosphorylates CDK1 and stimulates its kinase activity (PubMed : 20360007). Also dephosphorylates CDK2 in complex with cyclin-E, in vitro (PubMed : 20360007).

See full target information CDC25A pS124

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

European urology focus 7:1121-1129 PubMed33011150

2020

Comprehensive Analysis of the ATP-binding Cassette Subfamily B Across Renal Cancers Identifies ABCB8 Overexpression in Phenotypically Aggressive Clear Cell Renal Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Iulia Blajan,Herdis Miersch,Doris Schmidt,Glen Kristiansen,Sven Perner,Manuel Ritter,Jörg Ellinger,Niklas Klümper

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cdc25A (phospho S124) antibody

Western blot - Anti-Cdc25A (phospho S124) antibody (AB182666)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Primary Antibodies

Primary Antibodies

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Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise