AB249269

Anti-Cdc25A (phospho S124) antibody [EPR8888] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal Cdc25A phospho S124 antibody. Carrier free. Suitable for IHC-P, IP, WB and reacts with Human samples.

View Alternative Names

M-phase inducer phosphatase 1, Dual specificity phosphatase Cdc25A, CDC25A

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc25A (phospho S124) antibody [EPR8888] - BSA and Azide free (AB249269)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc25A (phospho S124) antibody [EPR8888] - BSA and Azide free (AB249269)

This data was developed using ab156574, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human testis tissue labeling Cdc25A (phospho S124) with ab156574 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc25A (phospho S124) antibody [EPR8888] - BSA and Azide free (AB249269)

This data was developed using ab156574, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human gastric carcinoma tissue labeling Cdc25A (phospho S124) with ab156574 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Immunoprecipitation - Anti-Cdc25A (phospho S124) antibody [EPR8888] - BSA and Azide free (AB249269)

This data was developed using ab156574, the same antibody clone in a different buffer formulation.ab156574, at a 1/1000 dilution, staining Cdc25A (phospho S124) in HeLa lysate immunoprecipitated using ab156574 at 1/10 dilution.

All lanes:

Immunoprecipitation - Anti-Cdc25A (phospho S124) antibody [EPR8888] (<a href='/en-us/products/primary-antibodies/cdc25a-phospho-s124-antibody-epr8888-ab156574'>ab156574</a>)

Predicted band size: 59 kDa

false

Lab

Western blot - Anti-Cdc25A (phospho S124) antibody [EPR8888] - BSA and Azide free (AB249269)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

We recommend use fresh lysate to get desired WB result.

This data was developed using ab156574, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Cdc25A (phospho S124) antibody [EPR8888] (<a href='/en-us/products/primary-antibodies/cdc25a-phospho-s124-antibody-epr8888-ab156574'>ab156574</a>) at 1/1000 dilution

Lane 1:

Jurkat (Human T cell leukemia T lymphocyte) whole cell fresh lysate at 20 µg

Lane 2:

Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate at 20 µg

Lane 3:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell fresh lysate at 20 µg

Lane 4:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 59 kDa

Observed band size: 70 kDa

false

Exposure time: 40s

Unconjugated

Anti-Cdc25A (phospho S124) antibody [EPR8888]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8888

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

ab249269 is the carrier-free version of ab156574.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cdc25A also known as Cell Division Cycle 25A acts as a phosphatase that removes phosphate groups from cyclin-dependent kinases (CDKs). This process mainly activates CDKs which are important for the cell cycle progression. Cdc25A has a molecular mass of approximately 65 kDa. The protein expresses in various cell types but finds higher levels in proliferative tissues. Additionally it localizes primarily in the nucleus where it executes its function during specific cell cycle phases.

Biological function summary

Cdc25A plays a big role in controlling transitions between cell cycle phases particularly the G1 to S phase. It participates in a CDK complex which includes partners like Cyclin E and Cyclin A driving progression through cell cycle checkpoints. During DNA replication Cdc25A's activity ensures that cells duplicate accurately maintaining genome integrity and balance in cell proliferation.

Pathways

Cdc25A functions within the cell cycle regulation and DNA damage repair pathways. It interacts importantly with proteins like Checkpoint Kinase 1 and 2 (Chk1/Chk2) involved in responding to DNA damage. During adverse conditions Chk1/Chk2 phosphorylate Cdc25A leading to its degradation and preventing further cycle progression. This mechanism helps maintain genomic stability by halting the cycle until DNA repair occurs.

Cdc25A holds connections to cancer and neurodegenerative conditions. Its overexpression often links to cancers as uncontrolled activity allows for unscheduled cell proliferation. Similarly dysregulation of Cdc25A relates to proteins like p53 which suppress tumor growth. In neurodegenerative disorders Cdc25A's misregulation can also increase the potential for neuronal cell cycle re-entry contributing to neuronal death. Understanding these relationships is essential for developing therapeutic targets.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression (PubMed : 12676925, PubMed : 14559997, PubMed : 1836978, PubMed : 20360007). Directly dephosphorylates CDK1 and stimulates its kinase activity (PubMed : 20360007). Also dephosphorylates CDK2 in complex with cyclin-E, in vitro (PubMed : 20360007).

See full target information CDC25A pS124

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cdc25A (phospho S124) antibody [EPR8888] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc25A (phospho S124) antibody [EPR8888] - BSA and Azide free (AB249269)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc25A (phospho S124) antibody [EPR8888] - BSA and Azide free (AB249269)

Immunoprecipitation - Anti-Cdc25A (phospho S124) antibody [EPR8888] - BSA and Azide free (AB249269)

Western blot - Anti-Cdc25A (phospho S124) antibody [EPR8888] - BSA and Azide free (AB249269)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise