Anti-Cdc25A (phospho S18) antibody [RP23040112]

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Cdc25A (phospho S18) antibody [RP23040112] (AB313439)

Immunofluorescent analysis of HeLa cells fixed using 4% formaldehyde (reconstituted in 1X PBS) for 10 min at room temperature and permeabilized using 0.1 % Triton X-100 in PBS for 15 min at room temperature treated with UV (302nM) for 60 minutes labeling Cdc25apS18 with ab313439 at 2 µg/mL followed by Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 488 conjugate at 1/2000 dilution. Panel a) shows representative cells that were stained for detection and localization of Cdc25apS18 protein (green), Panel b) is stained for nuclei (blue) using DAPI. Panel c) represents cytoskeletal F-actin staining using Alexa Fluor 555 Rhodamine Phalloidin at 1/300 dilution. Panel d) is a composite image of Panels a, b and c clearly demonstrating cytoplasmic and nuclear localization of Cdc25apS18. Panel e) represents composite image of untreated cells with no signal. Panel f) represents control cells with no primary antibody to assess background.

• WB

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Western blot - Anti-Cdc25A (phospho S18) antibody [RP23040112] (AB313439)

All lanes:

Western blot - Anti-Cdc25A (phospho S18) antibody [RP23040112] (ab313439) at 1 µg/mL

Lane 1:

HCT116 cell lysate at 30 µg

Lane 2:

HCT116 cell lysate treated with UV (For 40 min) at 30 µg

Lane 3:

HeLa cell lysate at 30 µg

Lane 4:

HeLa cell lysate treated with UV (For 40 min) at 30 µg

Lane 5:

HeLa cell lysate treated with Nocodazole at 30 µg

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/2500 dilution

Predicted band size: 59.087 kDa

Observed band size: 60 kDa

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