AB75743

Anti-Cdc25A (phospho S76) antibody

Name: Anti-Cdc25A (phospho S76) antibody (ab75743) | Abcam
Brand: Abcam Limited
SKU: AB75743
Price: 535 USD
Availability: InStock

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(7 Publications)

Rabbit Polyclonal Cdc25A phospho S76 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human CDC25A pS76.

View Alternative Names

M-phase inducer phosphatase 1, Dual specificity phosphatase Cdc25A, CDC25A

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc25A (phospho S76) antibody (AB75743)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc25A (phospho S76) antibody (AB75743)

ab75743, at a 1/50 dilution, staining Cdc25A in paraffin embedded human breast carcinoma tissue by Immunohistochemistry, in the absence (left image) or presence (right image) of the immunizing phospho peptide.

Unknown

Western blot - Anti-Cdc25A (phospho S76) antibody (AB75743)

All lanes:

Western blot - Anti-Cdc25A (phospho S76) antibody (ab75743) at 1/500 dilution

Lane 1:

A2780 cell extracts, untreated

Lane 2:

A2780 cell extracts, treated with UV

Predicted band size: 59 kDa

Observed band size: 30 kDa,59 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Mouse, Rat

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human CDC25A pS76. The exact immunogen used to generate this antibody is proprietary information.

P30304

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab75743 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cdc25A also known as Cell Division Cycle 25A acts as a phosphatase that removes phosphate groups from cyclin-dependent kinases (CDKs). This process mainly activates CDKs which are important for the cell cycle progression. Cdc25A has a molecular mass of approximately 65 kDa. The protein expresses in various cell types but finds higher levels in proliferative tissues. Additionally it localizes primarily in the nucleus where it executes its function during specific cell cycle phases.

Biological function summary

Cdc25A plays a big role in controlling transitions between cell cycle phases particularly the G1 to S phase. It participates in a CDK complex which includes partners like Cyclin E and Cyclin A driving progression through cell cycle checkpoints. During DNA replication Cdc25A's activity ensures that cells duplicate accurately maintaining genome integrity and balance in cell proliferation.

Pathways

Cdc25A functions within the cell cycle regulation and DNA damage repair pathways. It interacts importantly with proteins like Checkpoint Kinase 1 and 2 (Chk1/Chk2) involved in responding to DNA damage. During adverse conditions Chk1/Chk2 phosphorylate Cdc25A leading to its degradation and preventing further cycle progression. This mechanism helps maintain genomic stability by halting the cycle until DNA repair occurs.

Cdc25A holds connections to cancer and neurodegenerative conditions. Its overexpression often links to cancers as uncontrolled activity allows for unscheduled cell proliferation. Similarly dysregulation of Cdc25A relates to proteins like p53 which suppress tumor growth. In neurodegenerative disorders Cdc25A's misregulation can also increase the potential for neuronal cell cycle re-entry contributing to neuronal death. Understanding these relationships is essential for developing therapeutic targets.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression (PubMed : 12676925, PubMed : 14559997, PubMed : 1836978, PubMed : 20360007). Directly dephosphorylates CDK1 and stimulates its kinase activity (PubMed : 20360007). Also dephosphorylates CDK2 in complex with cyclin-E, in vitro (PubMed : 20360007).

See full target information CDC25A pS76

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

International journal of oncology 57:697-706 PubMed32582992

2020

Comparison of the therapeutic effects of lobaplatin and carboplatin on retinoblastoma in vitro and in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Zijun Zhou,Hua Jiang,Jiejun Xia,Jing Zhang

Journal of cellular physiology 234:3526-3537 PubMed30443946

2018

MicroRNA-99a-5p suppresses breast cancer progression and cell-cycle pathway through downregulating CDC25A.

Applications

Unspecified application

Species

Unspecified reactive species

Hongzhen Qin,Wenfei Liu

Molecular medicine reports 18:1345-1352 PubMed29901196

2018

Telomerase reverse transcriptase induced thyroid carcinoma cell proliferation through PTEN/AKT signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Zhang,Ning Hu

International journal of molecular medicine 41:3105-3114 PubMed29532862

2018

High glucose induces the proliferation of prostatic cells via downregulating MRE11.

Applications

Unspecified application

Species

Unspecified reactive species

Chunwei Ye,Yi Cai,Qian Cai,Shunhui Yuan,Fan Huang,Xiaofang Yang,Shuchen He,Zhuoheng Li,Yanwen Wang,Delin Yang,Zhipeng Li

Genes & cancer 8:505-516 PubMed28680535

2017

Ciclopirox inhibits cancer cell proliferation by suppression of Cdc25A.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Shen,Chaowei Shang,Hongyu Zhou,Yan Luo,Mansoureh Barzegar,Yoshinobu Odaka,Yang Wu,Shile Huang

Molecular medicine reports 13:1172-8 PubMed26647881

2015

Identification of key genes in hepatocellular carcinoma and validation of the candidate gene, cdc25a, using gene set enrichment analysis, meta-analysis and cross-species comparison.

Applications

Species

Unspecified reactive species

Xiaoxu Lu,Wen Sun,Yanping Tang,Lingqun Zhu,Yuan Li,Chao Ou,Chun Yang,Jianjia Su,Chengpiao Luo,Yanling Hu,Ji Cao

The EMBO journal 29:2802-12 PubMed20639859

2010

14-3-3gamma mediates Cdc25A proteolysis to block premature mitotic entry after DNA damage.

Applications

Species

Human

Kousuke Kasahara,Hidemasa Goto,Masato Enomoto,Yasuko Tomono,Tohru Kiyono,Masaki Inagaki

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cdc25A (phospho S76) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc25A (phospho S76) antibody (AB75743)

Western blot - Anti-Cdc25A (phospho S76) antibody (AB75743)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

Product promise