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AB167347

Anti-Cdc25B antibody

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(1 Publication)

Mouse Polyclonal Cdc25B antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CDC25B.

View Alternative Names

CDC25HU2, CDC25B, M-phase inducer phosphatase 2, Dual specificity phosphatase Cdc25B

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Cdc25B antibody (AB167347)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Cdc25B antibody (AB167347)

Immunofluorescent analysis of HeLa cells labeling Cdc25B with ab167347 at 10 µg/ml.

Western blot - Anti-Cdc25B antibody (AB167347)
  • WB

Unknown

Western blot - Anti-Cdc25B antibody (AB167347)

All lanes:

Western blot - Anti-Cdc25B antibody (ab167347) at 1 µg/mL

Lane 1:

Cdc25B transfected 293T cell line lysate at 15 µL

Lane 2:

Non-transfected 293T cell line lysate at 15 µL

Predicted band size: 64 kDa

false

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human CDC25B.

P30305

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Cdc25B phosphatase also known as M-phase inducer phosphatase 2 or CDC25B2 plays an important role in cell cycle regulation by activating cyclin-dependent kinases (CDKs). It weighs approximately 62 kDa. Researchers observe its expression in normal and cancerous tissues with higher levels found in proliferating cells. Cdc25B localizes to the nucleus during the cell cycle where it exerts control over cell division by removing inhibitory phosphates on CDKs.
Biological function summary

This protein accelerates the transition through various phases of the cell cycle. It does not function alone but forms part of a complex regulatory network ensuring timely CDK activation. By promoting the G1/S and G2/M transitions Cdc25B enables cells to progress through the cell cycle. Additionally Cdc25B is involved in DNA damage checkpoints helping cells respond to genotoxic stress thereby ensuring genomic stability through complex regulatory interactions.

Pathways

Several key cell cycle pathways include Cdc25B. It acts prominently in the cell cycle checkpoint control pathway and the DNA damage response pathway. The protein directly activates CDC2/CDK1 through dephosphorylation contributing to cell cycle progression. Cdc25B interacts with regulatory proteins like ATM/ATR which modulate Cdc25B activity in response to DNA damage further integrating it into critical checkpoint pathways.

Researchers link Cdc25B to cancer and neurological disorders. Overexpression of Cdc25B often associates with various cancers where it may contribute to unchecked cell proliferation through its influence on CDK activity. In this context it connects with oncogenic proteins like Cyclin B1 providing potential targets for therapeutic intervention. Additionally malfunction in Cdc25B activity associates with neurodegenerative diseases due to its role in cell cycle regulation and DNA repair mechanisms. Scientists are exploring its relationship with proteins such as p53 in these diseases offering insights into potential treatment strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression (PubMed : 1836978, PubMed : 20360007). Directly dephosphorylates CDK1 and stimulates its kinase activity (PubMed : 20360007). Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner (PubMed : 17332740). The three isoforms seem to have a different level of activity (PubMed : 1836978).
See full target information CDC25B
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

PloS one 9:e113138 PubMed25401926

2014

β-Arrestin-dependent deactivation of mouse melanopsin.

Applications

Unspecified application

Species

Unspecified reactive species

Evan G Cameron,Phyllis R Robinson
View all publications
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Product promise

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