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AB124819

Anti-Cdc25B antibody [EPR3459(2)]

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(8 Publications)

Rabbit Recombinant Monoclonal Cdc25B antibody. Suitable for IP, WB and reacts with Mouse, Human samples. Cited in 8 publications.

View Alternative Names

CDC25HU2, CDC25B, M-phase inducer phosphatase 2, Dual specificity phosphatase Cdc25B

2 Images
Western blot - Anti-Cdc25B antibody [EPR3459(2)] (AB124819)
  • WB

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Western blot - Anti-Cdc25B antibody [EPR3459(2)] (AB124819)

All lanes:

Western blot - Anti-Cdc25B antibody [EPR3459(2)] (ab124819) at 1/1000 dilution

Lane 1:

K562 cell lysate at 10 µg

Lane 2:

U937 cell lysate at 10 µg

Lane 3:

THP-1 cell lysate at 10 µg

Secondary

All lanes:

Goat-anti-rabbit HRP at 1/2000 dilution

Predicted band size: 64 kDa

Observed band size: 64 kDa

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Western blot - Anti-Cdc25B antibody [EPR3459(2)] (AB124819)
  • WB

Unknown

Western blot - Anti-Cdc25B antibody [EPR3459(2)] (AB124819)

Blocking/diluting Buffer and concentration : 5% NFDM /TBST

All lanes:

Western blot - Anti-Cdc25B antibody [EPR3459(2)] (ab124819) at 0.486 µg/mL

Lane 1:

NIH/3T3 (Mouse embryonic fibroblast) cell lysates at 20 µg

Lane 2:

Mouse embryo E17 lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 0.05 µg/mL

Predicted band size: 64 kDa

Observed band size: 64 kDa

false

  • Carrier free

    Anti-Cdc25B antibody [EPR3459(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3459(2)

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" } } }
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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Cdc25B phosphatase also known as M-phase inducer phosphatase 2 or CDC25B2 plays an important role in cell cycle regulation by activating cyclin-dependent kinases (CDKs). It weighs approximately 62 kDa. Researchers observe its expression in normal and cancerous tissues with higher levels found in proliferating cells. Cdc25B localizes to the nucleus during the cell cycle where it exerts control over cell division by removing inhibitory phosphates on CDKs.
Biological function summary

This protein accelerates the transition through various phases of the cell cycle. It does not function alone but forms part of a complex regulatory network ensuring timely CDK activation. By promoting the G1/S and G2/M transitions Cdc25B enables cells to progress through the cell cycle. Additionally Cdc25B is involved in DNA damage checkpoints helping cells respond to genotoxic stress thereby ensuring genomic stability through complex regulatory interactions.

Pathways

Several key cell cycle pathways include Cdc25B. It acts prominently in the cell cycle checkpoint control pathway and the DNA damage response pathway. The protein directly activates CDC2/CDK1 through dephosphorylation contributing to cell cycle progression. Cdc25B interacts with regulatory proteins like ATM/ATR which modulate Cdc25B activity in response to DNA damage further integrating it into critical checkpoint pathways.

Researchers link Cdc25B to cancer and neurological disorders. Overexpression of Cdc25B often associates with various cancers where it may contribute to unchecked cell proliferation through its influence on CDK activity. In this context it connects with oncogenic proteins like Cyclin B1 providing potential targets for therapeutic intervention. Additionally malfunction in Cdc25B activity associates with neurodegenerative diseases due to its role in cell cycle regulation and DNA repair mechanisms. Scientists are exploring its relationship with proteins such as p53 in these diseases offering insights into potential treatment strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression (PubMed : 1836978, PubMed : 20360007). Directly dephosphorylates CDK1 and stimulates its kinase activity (PubMed : 20360007). Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner (PubMed : 17332740). The three isoforms seem to have a different level of activity (PubMed : 1836978).
See full target information CDC25B
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

CNS neuroscience & therapeutics 31:e70172 PubMed39753851

2025

Integrated Mendelian Randomization and Single-Cell Transcriptomics Analysis Identifies Critical Blood Biomarkers and Potential Mechanisms in Epilepsy.

Applications

Unspecified application

Species

Unspecified reactive species

Jianwei Shi,Jing Xie,Yanfeng Yang,Bin Fu,Zuliang Ye,Ting Tang,Quanlei Liu,Jinkun Xu,Penghu Wei,Yongzhi Shan,Guoguang Zhao

The Journal of biological chemistry 299:102957 PubMed36717077

2023

Cyclin A-CDK1 suppresses the expression of the CDK1 activator CDC25A to safeguard timely mitotic entry.

Applications

Unspecified application

Species

Unspecified reactive species

Lau Yan Ng,Hoi Tang Ma,Randy Y C Poon

Journal of experimental & clinical cancer research : CR 41:185 PubMed35624501

2022

SOCS7/HuR/FOXM1 signaling axis inhibited high-grade serous ovarian carcinoma progression.

Applications

Unspecified application

Species

Unspecified reactive species

Yanhua Du,Xiang Xu,Siang Lv,Han Liu,Hong Sun,Jun Wu

Communications biology 5:39 PubMed35017636

2022

The metabolic stress-activated checkpoint LKB1-MARK3 axis acts as a tumor suppressor in high-grade serous ovarian carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Hidenori Machino,Syuzo Kaneko,Masaaki Komatsu,Noriko Ikawa,Ken Asada,Ryuichiro Nakato,Kanto Shozu,Ai Dozen,Kenbun Sone,Hiroshi Yoshida,Tomoyasu Kato,Katsutoshi Oda,Yutaka Osuga,Tomoyuki Fujii,Gottfried von Keudell,Vassiliki Saloura,Ryuji Hamamoto

Toxicology research 10:875-884 PubMed34484679

2021

is transcriptionally inhibited by through the transcription factor in irradiation-treated HeLa cells.

Applications

Unspecified application

Species

Unspecified reactive species

Lixin Ding,Xianzhe Zhao,Qiang Xiong,Xiaoyan Jiang,Xiaodan Liu,Kuke Ding,Pingkun Zhou

Molecular medicine reports 23: PubMed33355363

2020

lncRNA LIFR‑AS1 inhibits gastric carcinoma cell proliferation, migration and invasion by sponging miR‑4698.

Applications

Unspecified application

Species

Unspecified reactive species

Jiangqiao Zhao,Xiaoning Li,Liping Fu,Na Zhang,Jiaping Yang,Jianhui Cai

Oncology reports 39:2777-2786 PubMed29620242

2018

Corosolic acid induces cell cycle arrest and cell apoptosis in human retinoblastoma Y-79 cells via disruption of MELK-FoxM1 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Ke Wang,Xue Zhu,Yong Yao,Min Yang,Fanfan Zhou,Ling Zhu

Oncogene 36:6177-6189 PubMed28869606

2017

KCTD12 promotes tumorigenesis by facilitating CDC25B/CDK1/Aurora A-dependent G2/M transition.

Applications

Unspecified application

Species

Unspecified reactive species

Y Zhong,J Yang,W W Xu,Y Wang,C-C Zheng,B Li,Q-Y He
View all publications
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Product promise

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