JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB248020

Anti-Cdc25B antibody [EPR3459(2)] - BSA and Azide free

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Recombinant Monoclonal Cdc25B antibody. Carrier free. Suitable for IP, WB and reacts with Mouse, Human samples. Cited in 1 publication.

View Alternative Names

CDC25HU2, CDC25B, M-phase inducer phosphatase 2, Dual specificity phosphatase Cdc25B

2 Images
Western blot - Anti-Cdc25B antibody [EPR3459(2)] - BSA and Azide free (AB248020)
  • WB

Unknown

Western blot - Anti-Cdc25B antibody [EPR3459(2)] - BSA and Azide free (AB248020)

This data was developed using ab124819, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Cdc25B antibody [EPR3459(2)] (<a href='/en-us/products/primary-antibodies/cdc25b-antibody-epr34592-ab124819'>ab124819</a>) at 1/1000 dilution

Lane 1:

K562 cell lysate at 10 µg

Lane 2:

U937 cell lysate at 10 µg

Lane 3:

THP-1 cell lysate at 10 µg

Secondary

All lanes:

Goat-anti-rabbit HRP at 1/2000 dilution

Predicted band size: 64 kDa

Observed band size: 64 kDa

false

Western blot - Anti-Cdc25B antibody [EPR3459(2)] - BSA and Azide free (AB248020)
  • WB

Unknown

Western blot - Anti-Cdc25B antibody [EPR3459(2)] - BSA and Azide free (AB248020)

This data was developed using ab124819, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Cdc25B antibody [EPR3459(2)] (<a href='/en-us/products/primary-antibodies/cdc25b-antibody-epr34592-ab124819'>ab124819</a>) at 0.486 µg/mL

Lane 1:

NIH/3T3 (Mouse embryonic fibroblast) cell lysates at 20 µg

Lane 2:

Mouse embryo E17 lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 0.05 µg/mL

Predicted band size: 64 kDa

Observed band size: 64 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3459(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Complementary Products

Primary Antibodies

AB156574

Anti-Cdc25A (phospho S124) antibody [EPR8888]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc25A (phospho S124) antibody [EPR8888] (AB156574)

  • 0
  • 1
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
Primary Antibodies

AB32444

Anti-Cdc25C antibody [E302]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc25C antibody [E302] (AB32444)

  • 4
  • 3
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
style
left-column

Product details

ab248020 is the carrier-free version of ab124819.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Cdc25B phosphatase also known as M-phase inducer phosphatase 2 or CDC25B2 plays an important role in cell cycle regulation by activating cyclin-dependent kinases (CDKs). It weighs approximately 62 kDa. Researchers observe its expression in normal and cancerous tissues with higher levels found in proliferating cells. Cdc25B localizes to the nucleus during the cell cycle where it exerts control over cell division by removing inhibitory phosphates on CDKs.
Biological function summary

This protein accelerates the transition through various phases of the cell cycle. It does not function alone but forms part of a complex regulatory network ensuring timely CDK activation. By promoting the G1/S and G2/M transitions Cdc25B enables cells to progress through the cell cycle. Additionally Cdc25B is involved in DNA damage checkpoints helping cells respond to genotoxic stress thereby ensuring genomic stability through complex regulatory interactions.

Pathways

Several key cell cycle pathways include Cdc25B. It acts prominently in the cell cycle checkpoint control pathway and the DNA damage response pathway. The protein directly activates CDC2/CDK1 through dephosphorylation contributing to cell cycle progression. Cdc25B interacts with regulatory proteins like ATM/ATR which modulate Cdc25B activity in response to DNA damage further integrating it into critical checkpoint pathways.

Researchers link Cdc25B to cancer and neurological disorders. Overexpression of Cdc25B often associates with various cancers where it may contribute to unchecked cell proliferation through its influence on CDK activity. In this context it connects with oncogenic proteins like Cyclin B1 providing potential targets for therapeutic intervention. Additionally malfunction in Cdc25B activity associates with neurodegenerative diseases due to its role in cell cycle regulation and DNA repair mechanisms. Scientists are exploring its relationship with proteins such as p53 in these diseases offering insights into potential treatment strategies.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression (PubMed : 1836978, PubMed : 20360007). Directly dephosphorylates CDK1 and stimulates its kinase activity (PubMed : 20360007). Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner (PubMed : 17332740). The three isoforms seem to have a different level of activity (PubMed : 1836978).
See full target information CDC25B
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of virology 97:e0190722 PubMed36946735

2023

Characterization of the Impact of Merkel Cell Polyomavirus-Induced Interferon Signaling on Viral Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Ranran Wang,June F Yang,Taylor E Senay,Wei Liu,Jianxin You
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com