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Rabbit Recombinant Monoclonal Cdc25C antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications.

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Images

Western blot - Anti-Cdc25C antibody [E303] (AB32050), expandable thumbnail
  • Western blot - Anti-Cdc25C antibody [E303] (AB32050), expandable thumbnail
  • Western blot - Anti-Cdc25C antibody [E303] (AB32050), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHCWBICC/IF
Human
Not recommended
Tested
Not recommended

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Associated Products

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Target data

Function

Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle (PubMed:8119945). When phosphorylated, highly effective in activating G2 cells into prophase (PubMed:8119945). Directly dephosphorylates CDK1 and activates its kinase activity (PubMed:8119945).

Alternative names

M-phase inducer phosphatase 3, Dual specificity phosphatase Cdc25C, CDC25C

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Rabbit Recombinant Monoclonal Cdc25C antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
E303
Purification technique
Affinity purification Protein A
Specificity

ab32050 recognises Cdc25C. It does not cross react with other MPI phosphatase
family members.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Cdc25C also known as dual specificity phosphatase Cdc25C is a 65-kDa protein that acts as a cell cycle regulator controlling the transition from the G2 to M phase. The protein is expressed in various tissues but has higher expression levels in actively dividing cells. Cdc25C is important for cell division due to its ability to activate cyclin-dependent kinases (CDKs) by dephosphorylating the inactive phosphorylated forms. This action makes Cdc25C an important target for regulating the cell cycle and ensuring proper cell proliferation.

Biological function summary

The proper function of Cdc25C involves facilitating proper cell cycle progression by coordinating with other cell cycle regulators. It participates in a complex network where it dephosphorylates and activates CDK1/cyclin B1 complexes promoting the mitotic entry. This function is important for maintaining genomic stability during cell division. Misregulation of Cdc25C can lead to cell cycle arrest or uncontrolled cell proliferation highlighting its essential role in cell cycle control mechanisms.

Pathways

Cdc25C fits into the cell cycle checkpoint pathways and is also a part of the DNA damage response pathways. It connects with the p53 and ATM/ATR signaling proteins while responding to DNA damage ensuring a temporary pause in cell cycle progression for repair mechanisms to act. Proper interaction with these pathways is essential for maintaining cellular integrity and preventing the proliferation of damaged cells with CDK1 and Wee1 kinase serving as major interacting proteins in these processes.

Associated diseases and disorders

Misregulation or overexpression of Cdc25C can relate to cancer and various cell proliferation disorders. In cancer Cdc25C's disordered expression often links to unchecked cell division and tumor progression commonly involving proteins such as p53 which act as tumor suppressors. Furthermore abnormalities in Cdc25C function may lead to issues related to cell cycle progression errors implicating it in other proliferative disorders such as hyperplasia where it acts in conjunction with and alters the activities of proteins like cyclin B1.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-Cdc25C antibody [E303] (ab32050), expandable thumbnail

    Western blot - Anti-Cdc25C antibody [E303] (ab32050)

    Lanes 1- 2: Merged signal (red and green). Green - ab32050 observed at 58 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) observed at 37 kDa.

    ab32050 was shown to react with Cdc25C in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line Human CDC25C knockout HeLa cell line ab265189 (knockout cell lysate Human CDC25C knockout HeLa cell lysate ab257387) was used. Wild-type HeLa and CDC25C knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab32050 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-Cdc25C antibody [E303] (ab32050) at 1/1000 dilution

    Lane 1: Wild-type HeLa cell lysate at 20 µg

    Lane 2: CDC25C knockout HeLa cell lysate at 20 µg

    Lane 2: Western blot - Human CDC25C knockout HeLa cell line (Human CDC25C knockout HeLa cell line ab265189)

    Performed under reducing conditions.

    Predicted band size: 53 kDa

    Observed band size: 58 kDa

  • Western blot - Anti-Cdc25C antibody [E303] (ab32050), expandable thumbnail

    Western blot - Anti-Cdc25C antibody [E303] (ab32050)

    Lane 1: Wild-type HAP1 cell lysate (20 μg)
    Lane 2: Cdc25C knockout HAP1 cell lysate (20 μg)
    Lane 3: HeLa cell lysate (20 μg)
    Lane 4: Human bladder lysate (20 μg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab32050 observed at 58 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.
    ab32050 was shown to specifically react with Cdc25C when Cdc25C knockout samples were used. Wild-type and Cdc25C knockout samples were subjected to SDS-PAGE. ab32050 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10 000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    All lanes: Western blot - Anti-Cdc25C antibody [E303] (ab32050)

    Predicted band size: 53 kDa

  • Western blot - Anti-Cdc25C antibody [E303] (ab32050), expandable thumbnail

    Western blot - Anti-Cdc25C antibody [E303] (ab32050)

    All lanes: Western blot - Anti-Cdc25C antibody [E303] (ab32050) at 1/1000 dilution

    All lanes: A431 cell lysate

    Predicted band size: 53 kDa

    Observed band size: 54 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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