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AB96346

Anti-Cdc26 / Apc12 antibody

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(1 Publication)

Rabbit Polyclonal Cdc26 / Apc12 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CDC26 aa 1 to C-terminus.

View Alternative Names

ANAPC12, C9orf17, CDC26, Anaphase-promoting complex subunit CDC26, Anaphase-promoting complex subunit 12, Cell division cycle protein 26 homolog, APC12

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Cdc26 / Apc12 antibody (AB96346)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Cdc26 / Apc12 antibody (AB96346)

Immunofluorescence analysis of paraformaldehyde-fixed A431 cells, using ab96346 at 1/200 dilution. Lower image merged with DNA probe.

Western blot - Anti-Cdc26 / Apc12 antibody (AB96346)
  • WB

Unknown

Western blot - Anti-Cdc26 / Apc12 antibody (AB96346)

15% SDS PAGE

All lanes:

Western blot - Anti-Cdc26 / Apc12 antibody (ab96346) at 1/1000 dilution

All lanes:

H1299 whole cell lysate at 30 µg

Predicted band size: 10 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human CDC26 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q8NHZ8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cdc26 also known as Apc12 is a protein that plays a mechanical role as a subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C). This protein complex is essential for cell cycle regulation especially during the transition from metaphase to anaphase. Cdc26 has a mass of approximately 90 kDa. Expression of Cdc26 occurs mainly in dividing cells indicating its involvement in processes requiring tight cell cycle control.
Biological function summary

Cdc26/Apc12 functions as a component of the APC/C which is important for ubiquitin-mediated proteolysis of cell cycle proteins. As part of this large multi-subunit complex it aids the degradation of securin and cyclins which are proteins needed for cell cycle progression. The activity of Cdc26 is necessary to ensure correct chromosome separation during mitosis making it fundamental for maintaining genomic stability.

Pathways

Cdc26 integrates into cell cycle regulatory pathways particularly in the mitotic exit and G1 maintenance. It collaborates closely with other proteins such as Cdc20 and Cdh1 which together regulate the activity of the APC/C complex. These interactions highlight its role within the ubiquitin-proteasome signaling pathway and highlight its importance in maintaining orderly progression through the cell cycle.

Cdc26 is implicated in cancer due to its critical function in cell cycle regulation and maintenance of genomic integrity. Aberrant expression or malfunction of Cdc26 may lead to uncontrolled cell division contributing to tumorigenesis. Other proteins related to Cdc26 in cancer pathways like Cyclin B1 also play significant roles in uncontrolled cell proliferation. Additionally Cdc26 has associations with genetic disorders affecting cell division further underlining its significance in healthy cellular function.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle (PubMed : 18485873). The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins : it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (PubMed : 18485873). The APC/C complex catalyzes assembly of branched 'Lys-11'-/'Lys-48'-linked branched ubiquitin chains on target proteins (PubMed : 29033132). May recruit the E2 ubiquitin-conjugating enzymes to the complex (PubMed : 18485873).
See full target information CDC26
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

PloS one 10:e0118662 PubMed25723520

2015

LATS1 and LATS2 phosphorylate CDC26 to modulate assembly of the tetratricopeptide repeat subcomplex of APC/C.

Applications

Unspecified application

Species

Unspecified reactive species

Kenta Masuda,Tatsuyuki Chiyoda,Naoyuki Sugiyama,Aldo Segura-Cabrera,Yasuaki Kabe,Arisa Ueki,Kouji Banno,Koji Banno,Makoto Suematsu,Daisuke Aoki,Yasushi Ishihama,Hideyuki Saya,Shinji Kuninaka
View all publications
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