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AB72214

Anti-Cdc27 antibody

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(1 Publication)

Rabbit Polyclonal CDC27 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human CDC27.

View Alternative Names

ANAPC3, D0S1430E, D17S978E, CDC27, Cell division cycle protein 27 homolog, Anaphase-promoting complex subunit 3, CDC27 homolog, H-NUC, APC3, CDC27Hs

2 Images
Immunoprecipitation - Anti-Cdc27 antibody (AB72214)
  • IP

Unknown

Immunoprecipitation - Anti-Cdc27 antibody (AB72214)

Detection of Human Cdc27 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab72214 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.

All lanes:

Immunoprecipitation - Anti-Cdc27 antibody (ab72214)

Predicted band size: 91 kDa

false

Western blot - Anti-Cdc27 antibody (AB72214)
  • WB

Unknown

Western blot - Anti-Cdc27 antibody (AB72214)

All lanes:

Western blot - Anti-Cdc27 antibody (ab72214) at 0.04 µg/mL

Lane 1:

Whole cell lysate from HeLa cells at 50 µg

Lane 2:

Whole cell lysate from HeLa cells at 15 µg

Lane 3:

Whole cell lysate from HeLa cells at 5 µg

Lane 4:

Whole cell lysate from 293T cells at 50 µg

Predicted band size: 91 kDa

Observed band size: 100 kDa,45 kDa,50 kDa,70 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human CDC27. The exact immunogen used to generate this antibody is proprietary information.

P30260

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cdc27 also known as cell division cycle 27 or APC3 plays an important role in cell cycle regulation. It weighs approximately 92 kDa and is expressed in various tissues although it is found predominantly within the nucleus of proliferating cells. Cdc27 is a component of the anaphase-promoting complex/cyclosome (APC/C) which is vital for progression through mitosis and the G1 phase of the cell cycle by targeting specific proteins for degradation.
Biological function summary

Cdc27 serves as an essential part of the APC/C a large E3 ubiquitin ligase complex. By forming the scaffold for other subunits Cdc27 helps regulate the ubiquitination and subsequent degradation of mitotic cyclins and other proteins involved in cell cycle progression. The interaction of Cdc27 with other proteins like Cdc16 and Cdc23 stabilizes the APC/C complex ensuring its proper function.

Pathways

Cdc27 participates actively in the cell cycle especially during the G2/M transition and mitotic exit. It is involved in the ubiquitin-proteasome pathway where it intersects with proteins like securin and cyclin B1. These interactions allow Cdc27 to mediate the transition between cell cycle phases and support chromosome segregation.

Disruptions in Cdc27 expression or function are linked to cancer development particularly breast and colorectal cancers. Abnormal activity in the APC/C complex can lead to uncontrolled cell proliferation. Cdc27 works alongside Cdh1 another APC/C co-activator protein highlighting its significance in maintaining cell cycle fidelity.unger.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle (PubMed : 18485873). The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins : it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (PubMed : 18485873). The APC/C complex catalyzes assembly of branched 'Lys-11'-/'Lys-48'-linked branched ubiquitin chains on target proteins (PubMed : 29033132).
See full target information CDC27
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Medical science monitor : international medical jo 21:1297-303 PubMed25943633

2015

MiR-27a modulates radiosensitivity of triple-negative breast cancer (TNBC) cells by targeting CDC27.

Applications

WB

Species

Human

Yong-qiang Ren,Fengkui Fu,Jianjun Han
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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