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AB129085

Anti-Cdc27 antibody [EPR7798]

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(4 Publications)

Rabbit Recombinant Monoclonal CDC27 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 4 publications.

View Alternative Names

ANAPC3, D0S1430E, D17S978E, CDC27, Cell division cycle protein 27 homolog, Anaphase-promoting complex subunit 3, CDC27 homolog, H-NUC, APC3, CDC27Hs

2 Images
Western blot - Anti-Cdc27 antibody [EPR7798] (AB129085)
  • WB

Unknown

Western blot - Anti-Cdc27 antibody [EPR7798] (AB129085)

All lanes:

Western blot - Anti-Cdc27 antibody [EPR7798] (ab129085) at 1/1000 dilution

Lane 1:

K562 cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 132 kDa,91 kDa

Observed band size: 132 kDa

false

OI-RD Scanning - Anti-Cdc27 antibody [EPR7798] (AB129085)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-Cdc27 antibody [EPR7798] (AB129085)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7798

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cdc27 also known as cell division cycle 27 or APC3 plays an important role in cell cycle regulation. It weighs approximately 92 kDa and is expressed in various tissues although it is found predominantly within the nucleus of proliferating cells. Cdc27 is a component of the anaphase-promoting complex/cyclosome (APC/C) which is vital for progression through mitosis and the G1 phase of the cell cycle by targeting specific proteins for degradation.
Biological function summary

Cdc27 serves as an essential part of the APC/C a large E3 ubiquitin ligase complex. By forming the scaffold for other subunits Cdc27 helps regulate the ubiquitination and subsequent degradation of mitotic cyclins and other proteins involved in cell cycle progression. The interaction of Cdc27 with other proteins like Cdc16 and Cdc23 stabilizes the APC/C complex ensuring its proper function.

Pathways

Cdc27 participates actively in the cell cycle especially during the G2/M transition and mitotic exit. It is involved in the ubiquitin-proteasome pathway where it intersects with proteins like securin and cyclin B1. These interactions allow Cdc27 to mediate the transition between cell cycle phases and support chromosome segregation.

Disruptions in Cdc27 expression or function are linked to cancer development particularly breast and colorectal cancers. Abnormal activity in the APC/C complex can lead to uncontrolled cell proliferation. Cdc27 works alongside Cdh1 another APC/C co-activator protein highlighting its significance in maintaining cell cycle fidelity.unger.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle (PubMed : 18485873). The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins : it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (PubMed : 18485873). The APC/C complex catalyzes assembly of branched 'Lys-11'-/'Lys-48'-linked branched ubiquitin chains on target proteins (PubMed : 29033132).
See full target information CDC27
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Ecotoxicology and environmental safety 256:114823 PubMed36989553

2023

miR-186 induces tetraploidy in arsenic exposed human keratinocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Ana P Ferragut Cardoso,Alexandra N Nail,Mayukh Banerjee,Sandra S Wise,J Christopher States

Nucleic acids research 48:3257-3276 PubMed31970406

2020

Genome-wide analysis reveals a switch in the translational program upon oocyte meiotic resumption.

Applications

Unspecified application

Species

Unspecified reactive species

Xuan G Luong,Enrico Maria Daldello,Gabriel Rajkovic,Cai-Rong Yang,Marco Conti

Toxicology and applied pharmacology 378:114614 PubMed31176655

2019

Overexpression of hsa-miR-186 induces chromosomal instability in arsenic-exposed human keratinocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Jiguo Wu,Ana P Ferragut Cardoso,Vanessa A R States,Laila Al-Eryani,Mark Doll,Sandra S Wise,Shesh N Rai,J Christopher States

Nature communications 7:13887 PubMed27976684

2016

Profiling DNA damage response following mitotic perturbations.

Applications

Unspecified application

Species

Unspecified reactive species

Ronni S Pedersen,Gopal Karemore,Thorkell Gudjonsson,Maj-Britt Rask,Beate Neumann,Jean-Karim Hériché,Rainer Pepperkok,Jan Ellenberg,Daniel W Gerlich,Jiri Lukas,Claudia Lukas
View all publications
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