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Rabbit Recombinant Monoclonal Cdc34 antibody. Carrier free. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Mouse, Human, Rat samples.

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Images

Western blot - Anti-Cdc34 antibody [EPR16808] - BSA and Azide free (AB251415), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Cdc34 antibody [EPR16808] - BSA and Azide free (AB251415), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Cdc34 antibody [EPR16808] - BSA and Azide free (AB251415), expandable thumbnail
  • Western blot - Anti-Cdc34 antibody [EPR16808] - BSA and Azide free (AB251415), expandable thumbnail
  • Western blot - Anti-Cdc34 antibody [EPR16808] - BSA and Azide free (AB251415), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPWBFlow Cyt (Intra)
Human
Tested
Tested
Tested
Tested
Mouse
Tested
Expected
Tested
Expected
Rat
Expected
Expected
Tested
Expected

Tested
Tested

Species
Mouse, Human
Dilution info
-
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Target data

Function

E2 ubiquitin-conjugating enzyme that accepts ubiquitin from an E1 ubiquitin-activating protein, and catalyzes its covalent attachment to other proteins by an E3 ubiquitin-protein ligase complex (PubMed:10329681, PubMed:17588522, PubMed:20061386, PubMed:38326650). In vitro catalyzes 'Lys-48'-linked polyubiquitination (PubMed:22496338). Cooperates with the E2 UBCH5C and the SCF(FBXW11) E3 ligase complex for the polyubiquitination of NFKBIA leading to its subsequent proteasomal degradation (PubMed:10329681, PubMed:10918611, PubMed:17698585). Performs ubiquitin chain elongation building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. UBE2D3 acts as an initiator E2, priming the phosphorylated NFKBIA target at positions 'Lys-21' and/or 'Lys-22' with a monoubiquitin. Cooperates with the SCF(SKP2) E3 ligase complex to regulate cell proliferation through ubiquitination and degradation of MYBL2 and KIP1 (PubMed:10871850, PubMed:15652359, PubMed:19112177). Involved in ubiquitin conjugation and degradation of CREM isoform ICERIIgamma and ATF15 resulting in abrogation of ICERIIgamma- and ATF5-mediated repression of cAMP-induced transcription during both meiotic and mitotic cell cycles. Involved in the regulation of the cell cycle G2/M phase through its targeting of the WEE1 kinase for ubiquitination and degradation (PubMed:19126550). Also involved in the degradation of beta-catenin (PubMed:12037680). Is target of human herpes virus 1 protein ICP0, leading to ICP0-dependent dynamic interaction with proteasomes (PubMed:11805320, PubMed:12060736).

Alternative names

UBCH3, UBE2R1, CDC34, Ubiquitin-conjugating enzyme E2 R1, (E3-independent) E2 ubiquitin-conjugating enzyme R1, E2 ubiquitin-conjugating enzyme R1, Ubiquitin-conjugating enzyme E2-32 kDa complementing, Ubiquitin-conjugating enzyme E2-CDC34, Ubiquitin-protein ligase R1

Recommended products

Rabbit Recombinant Monoclonal Cdc34 antibody. Carrier free. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Mouse, Human, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR16808
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab251415 is the carrier-free version of Anti-Cdc34 antibody [EPR16808] ab204515.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The Cdc34 protein also known as Ubc3 is a ubiquitin-conjugating enzyme with a molecular mass of about 35 kDa. It is expressed in various tissues with high presence in actively dividing cells. Cdc34 plays a significant role in cell cycle regulation particularly during the transition from G1 to the S phase. It facilitates the attachment of ubiquitin molecules to target proteins marking them for degradation by the proteasome which is important for controlling the abundance of specific proteins involved in cell cycle progression.

Biological function summary

Cdc34 functions as a member of the SCF (SKP1-CUL1-F-box) ubiquitin ligase complex. This complex targets a range of key regulatory proteins for ubiquitination influencing processes such as DNA replication and repair. By mediating protein degradation Cdc34 helps regulate the cellular levels of cyclins which are essential for proper cell cycle advancement. Its activity ensures timely degradation of these cyclins thereby preventing unscheduled cell cycle transitions that can lead to cellular dysfunction.

Pathways

Cdc34 is integrated in the ubiquitin-proteasome pathway and the cell cycle pathway. In these contexts Cdc34's activity closely interacts with proteins like Skp2 another component of the SCF complex which regulates G1-S phase transition. Cdc34-related pathways ensure that protein turnover is tightly controlled which is essential for maintaining cellular homeostasis and responding to internal and external signals during cell proliferation.

Associated diseases and disorders

Cdc34 links to cancer and neurodegenerative disorders. Alterations in Cdc34 function can result in the aberrant degradation of regulatory proteins contributing to unchecked cell proliferation a hallmark of cancer. Furthermore imbalances in ubiquitin-proteasome pathway components including Cdc34 relate to neurodegenerative diseases where protein aggregation occurs. Connections to proteins such as p27 which regulates cell cycle and accumulation issues in neurological tissues highlight the significance of Cdc34 in disease mechanisms.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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