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AB288379

Anti-Cdc37 antibody [10C3]

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Rabbit Recombinant Monoclonal CDC37 antibody. Suitable for Flow Cyt, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human CDC37.

View Alternative Names

CDC37A, CDC37, Hsp90 co-chaperone Cdc37, Hsp90 chaperone protein kinase-targeting subunit, p50Cdc37

2 Images
Flow Cytometry - Anti-Cdc37 antibody [10C3] (AB288379)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-Cdc37 antibody [10C3] (AB288379)

Overlay histogram showing HeLa cells stained with ab288379 (red line) at 1 : 50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1μg/1 x 106cells) for 1 h at 4°C.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30 min at 4°C. Control antibody (green line) was Rabbit IgG (1μg/1 x 106cells) used under the same conditions. Acquisition of >10,000 events was performed.

Western blot - Anti-Cdc37 antibody [10C3] (AB288379)
  • WB

Supplier Data

Western blot - Anti-Cdc37 antibody [10C3] (AB288379)

All lanes:

Western blot - Anti-Cdc37 antibody [10C3] (ab288379) at 1/2000 dilution

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Lane 2:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate

Lane 3:

MCF7 (Human breast adenocarcinoma cell line) whole cell lysate

Lane 4:

HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate

Lane 5:

K-562 (Human chronic myelogenous leukemia lymphoblast cell line ) whole cell lysate

Lane 6:

PC-3 (Human prostate adenocarcinoma cell line) whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 44 kDa

Observed band size: 50 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

10C3

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, Flow Cyt

applications

Immunogen

Synthetic Peptide within Human CDC37. The exact immunogen used to generate this antibody is proprietary information.

Q16543

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/50", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 48% PBS, 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Cdc37 protein also known as cell division cycle 37 homolog is a molecular chaperone that assists in the proper folding and stabilization of protein kinases. With a mass of approximately 44 kDa Cdc37 is widely expressed in mammalian tissues. It interacts directly with other proteins aiding in their stabilization and activity. Functioning closely with Hsp90 Cdc37 forms a chaperone complex critical for the maturation of several protein kinases.
Biological function summary

Cdc37 serves as a co-chaperone with Hsp90 in regulating protein kinase activity by maintaining their functional conformation and stability. This protein acts mainly as a kinase-specific adapter that recruits kinases to the Hsp90 chaperone system forming a protein complex essential for the proper functioning of key signaling molecules. Its role is vital in signal transduction and cellular growth regulation ensuring proper cell cycle progression and response to external stimuli.

Pathways

Cdc37 plays a significant role in the PI3K/AKT and MAPK signaling pathways. In these pathways Cdc37 assists in the proper activation and function of various kinases such as AKT and RAF which are important for cell growth survival and proliferation. Its partnership with Hsp90 in these pathways underlines its importance in maintaining kinase activity ensuring that signaling cascades proceed correctly for normal cellular function.

Cdc37 has been implicated in cancer and neurodegenerative diseases like Alzheimer's. Overexpression or dysfunction of Cdc37 can lead to improper activation of oncogenic kinases contributing to tumor progression. Additionally the protein's association with Hsp90 highlights its potential involvement in Alzheimer's where protein misfolding and stability are disrupted making Cdc37 a target of interest for therapeutic intervention in these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Co-chaperone that binds to numerous kinases and promotes their interaction with the Hsp90 complex, resulting in stabilization and promotion of their activity (PubMed : 8666233). Inhibits HSP90AA1 ATPase activity (PubMed : 23569206).
See full target information CDC37
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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