AB326655

Anti-CdC37 (phospho S13) antibody [SR1889]

Recombinant
What is this?

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Rabbit Recombinant Monoclonal CDC37 phospho S13 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples.

View Alternative Names

CDC37A, CDC37, Hsp90 co-chaperone Cdc37, Hsp90 chaperone protein kinase-targeting subunit, p50Cdc37

3 Images

Supplier Data

Western blot - Anti-CdC37 (phospho S13) antibody [SR1889] (AB326655)

All lanes : Primary antibody at 1/1000 dilution

Lane 1 : 3T3 whole cell lysates
Lane 2 : PC12 whole cell lysates
Lane 3 : 293 whole cell lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size : 44 kDa
Observed band size : 50 kDa

Exposure time : 7 seconds

All lanes:

Western blot - Anti-CdC37 (phospho S13) antibody [SR1889] (ab326655) at 1/1000 dilution

Lane 1:

3T3 whole cell lysates at 20 µg

Lane 2:

PC12 whole cell lysates at 20 µg

Lane 3:

293 whole cell lysates at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 44 kDa

Observed band size: 50 kDa

false

Exposure time: 7s

Supplier Data

Western blot - Anti-CdC37 (phospho S13) antibody [SR1889] (AB326655)

All lanes : Primary antibody at 1/1000 dilution

Lane 1 : JK treated with alkaline phosphatase whole cell lysates
Lane 2 : JK whole cell lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size : 44 kDa
Observed band size : 50 kDa

Exposure time : 4 seconds

All lanes:

Western blot - Anti-CdC37 (phospho S13) antibody [SR1889] (ab326655) at 1/1000 dilution

Lane 1:

JK treated with alkaline phosphatase whole cell lysates at 20 µg

Lane 2:

JK whole cell lysates at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 44 kDa

Observed band size: 50 kDa

false

Exposure time: 4s

Supplier Data

Western blot - Anti-CdC37 (phospho S13) antibody [SR1889] (AB326655)

All lanes : Primary antibody at 1/1000 dilution

Lane 1 : Wild-type Hela cell lysate
Lane 2 : CDC37 (Phospho-Ser13) knockdown Hela cell lysate

Lysates/proteins at 20 µg per lane.

All lanes:

Western blot - Anti-CdC37 (phospho S13) antibody [SR1889] (ab326655) at 1/1000 dilution

Lane 1:

Wild-type Hela cell lysate at 20 µg

Lane 2:

CdC37 (phospho S13) knockdown Hela cell lysate at 20 µg

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SR1889

Isotype

IgG

Carrier free

Reacts with

Human, Mouse, Rat

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "" } } }

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Ambient - Can Ship with Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Co-chaperone that binds to numerous kinases and promotes their interaction with the Hsp90 complex, resulting in stabilization and promotion of their activity (PubMed : 8666233). Inhibits HSP90AA1 ATPase activity (PubMed : 23569206).

See full target information CDC37 pS13

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com