AB175924

Anti-CDC40 antibody [EPR12539]

RabMAb
Recombinant
What is this?

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(4 Publications)

Rabbit Recombinant Monoclonal CDC40 antibody. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 4 publications.

View Alternative Names

EHB3, PRP17, PRPF17, CDC40, Pre-mRNA-processing factor 17, Cell division cycle 40 homolog, EH-binding protein 3, PRP17 homolog, Ehb3, hPRP17

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC40 antibody [EPR12539] (AB175924)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC40 antibody [EPR12539] (AB175924)

Immunohistochemical analysis of paraffin-embedded transitional cell carcinoma tissue labeling CDC40 with ab175924 at 1/50 dilution

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC40 antibody [EPR12539] (AB175924)

Immunohistochemical analysis of paraffin-embedded colon tissue using labeling CDC40 with ab175924 at 1/50 dilution

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-CDC40 antibody [EPR12539] (AB175924)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-CDC40 antibody [EPR12539] (AB175924)

Intracellular flow cytometric analysis of permeabilized K562 cells labeling CDC40 with ab175924 at 1/10 dilution (red) or a rabbit IgG (negative) (green).

Immunocytochemistry/ Immunofluorescence - Anti-CDC40 antibody [EPR12539] (AB175924)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CDC40 antibody [EPR12539] (AB175924)

Immunofluorescent staining of HeLa cells labeling CDC40 with ab175924 at 1/100 dilution.

Supplier Data

Western blot - Anti-CDC40 antibody [EPR12539] (AB175924)

All lanes:

Western blot - Anti-CDC40 antibody [EPR12539] (ab175924) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

K562 cell lysate at 10 µg

Lane 3:

293T cell lysate at 10 µg

Lane 4:

HUVEC cell lysate at 10 µg

Predicted band size: 66 kDa

false

Carrier free

Anti-CDC40 antibody [EPR12539] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12539

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P, Flow Cyt (Intra), ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Required for pre-mRNA splicing as component of the activated spliceosome (PubMed : 33220177). Plays an important role in embryonic brain development; this function does not require proline isomerization (PubMed : 33220177).

See full target information CDC40

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:315 PubMed39747150

2025

CDC40 suppression induces CDCA5 splicing defects and anti-proliferative effects in lung cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Die Hu,Brigitte L Thériault,Vida Talebian,Laurent Hoffer,Julie Owen,Justin Lim,Benjamin J Blencowe,Evelyne Lima-Fernandes,Punit Saraon,Richard Marcellus,Rima Al-Awar

PloS one 18:e0289327 PubMed37531400

2023

Dynamics of TERT regulation via alternative splicing in stem cells and cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jeongjin J Kim,Mohammed E Sayed,Alexander Ahn,Aaron L Slusher,Jeffrey Y Ying,Andrew T Ludlow

Neuron 109:241-256.e9 PubMed33220177

2020

Mutations in Spliceosomal Genes PPIL1 and PRP17 Cause Neurodegenerative Pontocerebellar Hypoplasia with Microcephaly.

Applications

Unspecified application

Species

Unspecified reactive species

Guoliang Chai,Alice Webb,Chen Li,Danny Antaki,Sangmoon Lee,Martin W Breuss,Nhi Lang,Valentina Stanley,Paula Anzenberg,Xiaoxu Yang,Trevor Marshall,Patrick Gaffney,Klaas J Wierenga,Brian Hon-Yin Chung,Mandy Ho-Yin Tsang,Lynn S Pais,Alysia Kern Lovgren,Grace E VanNoy,Heidi L Rehm,Ghayda Mirzaa,Eyby Leon,Jullianne Diaz,Alexander Neumann,Arnout P Kalverda,Iain W Manfield,David A Parry,Clare V Logan,Colin A Johnson,David T Bonthron,Elizabeth M A Valleley,Mahmoud Y Issa,Sherif F Abdel-Ghafar,Mohamed S Abdel-Hamid,Patricia Jennings,Maha S Zaki,Eamonn Sheridan,Joseph G Gleeson

Experimental and therapeutic medicine 13:1702-1710 PubMed28565756

2017

MicroRNA-98 rescues proliferation and alleviates ox-LDL-induced apoptosis in HUVECs by targeting LOX-1.

Applications

Unspecified application

Species

Unspecified reactive species

Zhibo Chen,Mian Wang,Qiong He,Zilun Li,Yang Zhao,Wenjian Wang,Jieyi Ma,Yongxin Li,Guangqi Chang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CDC40 antibody [EPR12539]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC40 antibody [EPR12539] (AB175924)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC40 antibody [EPR12539] (AB175924)

Flow Cytometry (Intracellular) - Anti-CDC40 antibody [EPR12539] (AB175924)

Immunocytochemistry/ Immunofluorescence - Anti-CDC40 antibody [EPR12539] (AB175924)

Western blot - Anti-CDC40 antibody [EPR12539] (AB175924)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (4)

Product promise