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AB255897

Anti-CDC42 antibody [28-10]

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(3 Publications)

Mouse Recombinant Monoclonal CDC42 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 3 publications.

View Alternative Names

Cell division control protein 42 homolog, G25K GTP-binding protein, CDC42

2 Images
Western blot - Anti-CDC42 antibody [28-10] (AB255897)
  • WB

Supplier Data

Western blot - Anti-CDC42 antibody [28-10] (AB255897)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure times : Lane 1 : 5 seconds; Lane 2-4 : 26 seconds.

All lanes:

Western blot - Anti-CDC42 antibody [28-10] (ab255897) at 0.544 µg/mL

Lane 1:

Jurkat (human T cell leukemia T lymphocyte), whole cell lysate at 20 µg

Lane 2:

THP1 (human monocytic leukemia monocyte), whole cell lysate at 20 µg

Lane 3:

NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 20 µg

Lane 4:

PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution

Predicted band size: 21 kDa

false

Western blot - Anti-CDC42 antibody [28-10] (AB255897)
  • WB

Supplier Data

Western blot - Anti-CDC42 antibody [28-10] (AB255897)

Blocking/Dilution buffer : 5% NFDM/TBST.

Lanes 1 and 3:

Western blot - Anti-CDC42 antibody [28-10] (ab255897) at 0.544 µg/mL

Lanes 2 and 4:

Western blot - Anti-CDC42 antibody [28-10] (ab255897) at 0.554 µg/mL

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Mouse spleen tissue lysate at 20 µg

Lane 3:

Rat brain tissue lysate at 20 µg

Lane 4:

Rat spleen tissue lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution

Predicted band size: 21 kDa

false

Exposure time: 3s

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

28-10

Isotype

IgG2b

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.544 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.544 µg/mL", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.544 µg/mL", "WB-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Regulates the bipolar attachment of spindle microtubules to kinetochores before chromosome congression in metaphase (PubMed : 15642749). Regulates cell migration (PubMed : 17038317, PubMed : 22843693). In neurons, plays a role in the extension and maintenance of the formation of filopodia, thin and actin-rich surface projections (PubMed : 14978216). Required for DOCK10-mediated spine formation in Purkinje cells and hippocampal neurons. In podocytes, facilitates filopodia and podosomes formation upon DOCK11-activation (PubMed : 33523862). Upon activation by CaMKII, modulates dendritic spine structural plasticity by relaying CaMKII transient activation to synapse-specific, long-term signaling (By similarity). Also plays a role in phagocytosis through organization of the F-actin cytoskeleton associated with forming phagocytic cups (PubMed : 26465210). Upon activation by PLEKHG4B, involved in actin cytoskeletal remodeling during epithelial cell-cell junction formation (PubMed : 33310911).
See full target information Cell division control protein 42 homolog
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cartilage :19476035251323601 PubMed40119525

2025

Transcriptional Analysis Reveals That the FHL1/JAK-STAT Pathway is Involved in Acute Cartilage Injury in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Lu,Zhenhua Shi,Lindan Geng,Dong Ren,Haowei Hou,Guowei Ren,Shuangquan Yao,Pengcheng Wang

The Journal of biological chemistry 291:26262-26272 PubMed27803162

2016

The Neural Cell Adhesion Molecule (NCAM) Promotes Clustering and Activation of EphA3 Receptors in GABAergic Interneurons to Induce Ras Homolog Gene Family, Member A (RhoA)/Rho-associated protein kinase (ROCK)-mediated Growth Cone Collapse.

Applications

Unspecified application

Species

Unspecified reactive species

Chelsea S Sullivan,Maike Kümper,Brenda S Temple,Patricia F Maness

Molecular and cellular biology 30:5207-17 PubMed20823269

2010

The function of two Rho family GTPases is determined by distinct patterns of cell surface localization.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Wu,Patrick Brennwald
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com