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AB155940

Anti-CDC42 antibody

4

(3 Reviews)

|

(16 Publications)

Rabbit Polyclonal CDC42 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, African green monkey, Rat, Primates samples. Cited in 16 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Cell division control protein 42 homolog.

View Alternative Names

Cell division control protein 42 homolog, G25K GTP-binding protein, CDC42

6 Images
Immunocytochemistry/ Immunofluorescence - Anti-CDC42 antibody (AB155940)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CDC42 antibody (AB155940)

Immunofluorescent analysis of A431 cells, labeling CDC42 with ab155940 at 1/20 dilution. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is also shown.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC42 antibody (AB155940)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC42 antibody (AB155940)

Immunohistochemical analysis of deparaffinised Human pancreas tissue, labeling CDC42 with ab155940 at 1/20 dilution. Detection was performed using a biotin conjugated secondary antibody and SA-HRP. Right image is immunohistochemistry performed without primary antibody (negative control).

Immunocytochemistry/ Immunofluorescence - Anti-CDC42 antibody (AB155940)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CDC42 antibody (AB155940)

Immunofluorescent analysis of HepG2 cells, labeling CDC42 with ab155940 at 1/200 dilution. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is also shown.

Immunocytochemistry/ Immunofluorescence - Anti-CDC42 antibody (AB155940)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CDC42 antibody (AB155940)

Immunofluorescent analysis of HeLa cells, labeling CDC42 with ab155940 at 1/100 dilution. Blue : Nuclei stained with Hoechst 33342.

Immunocytochemistry/ Immunofluorescence - Anti-CDC42 antibody (AB155940)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CDC42 antibody (AB155940)

Immunofluorescent analysis of C6 cells, labeling CDC42 with ab155940 at 1/20 dilution. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is also shown.

Western blot - Anti-CDC42 antibody (AB155940)
  • WB

Unknown

Western blot - Anti-CDC42 antibody (AB155940)

4-20% Tris HCl

All lanes:

Western blot - Anti-CDC42 antibody (ab155940) at 1/1000 dilution

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HepG2 whole cell lysate at 50 µg

Lane 3:

293T whole cell lysate at 50 µg

Lane 4:

A431 whole cell lysate at 50 µg

Lane 5:

Jurkat whole cell lysate at 50 µg

Lane 6:

MCF7 whole cell lysate at 50 µg

Lane 7:

U2-OS whole cell lysate at 50 µg

Lane 8:

K526 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate at 50 µg

Lane 9:

COS-7 (African green monkey kidney fibroblast-like cell line) whole cell lysate at 50 µg

Lane 10:

MEF whole cell lysate at 50 µg

Lane 11:

3T3L1 whole cell lysate at 50 µg

Predicted band size: 21 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human, Primates, African green monkey

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human Cell division control protein 42 homolog. The exact immunogen used to generate this antibody is proprietary information.

P60953

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" }, "African green monkey": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Primates": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/20", "IHCP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.05% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Regulates the bipolar attachment of spindle microtubules to kinetochores before chromosome congression in metaphase (PubMed : 15642749). Regulates cell migration (PubMed : 17038317, PubMed : 22843693). In neurons, plays a role in the extension and maintenance of the formation of filopodia, thin and actin-rich surface projections (PubMed : 14978216). Required for DOCK10-mediated spine formation in Purkinje cells and hippocampal neurons. In podocytes, facilitates filopodia and podosomes formation upon DOCK11-activation (PubMed : 33523862). Upon activation by CaMKII, modulates dendritic spine structural plasticity by relaying CaMKII transient activation to synapse-specific, long-term signaling (By similarity). Also plays a role in phagocytosis through organization of the F-actin cytoskeleton associated with forming phagocytic cups (PubMed : 26465210). Upon activation by PLEKHG4B, involved in actin cytoskeletal remodeling during epithelial cell-cell junction formation (PubMed : 33310911).
See full target information Cell division control protein 42 homolog
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Publications (16)

Recent publications for all applications. Explore the full list and refine your search

Evidence-based complementary and alternative medicine : eCAM 2022:7044208 PubMed36159559

2022

Electroacupuncture Enhances Cognitive Deficits in a Rat Model of Rapid Eye Movement Sleep Deprivation via Targeting MiR-132.

Applications

Unspecified application

Species

Unspecified reactive species

Li Hao,Yiming Wu,Jin Xie,Xinwang Chen

Cells 11: PubMed36078162

2022

Leptin Promotes Angiogenesis via Pericyte STAT3 Pathway upon Intracerebral Hemorrhage.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Cui,Yingmei Zhang,Ning Tian,Jiaxin Yang,Dongshan Ya,Wenjing Xiang,Zixian Zhou,Yanlin Jiang,Jungang Deng,Bin Yang,Xiaohui Lin,Qinghua Li,Rujia Liao

Nutrients 14: PubMed35893859

2022

Mulberry Leaf and Neochlorogenic Acid Alleviates Glucolipotoxicity-Induced Oxidative Stress and Inhibits Proliferation/Migration via Downregulating Ras and FAK Signaling Pathway in Vascular Smooth Muscle Cell.

Applications

Unspecified application

Species

Unspecified reactive species

Tsung-Yuan Yang,Yi-Liang Wu,Meng-Hsun Yu,Tung-Wei Hung,Kuei-Chuan Chan,Chau-Jong Wang

The EMBO journal 41:e109694 PubMed35694726

2022

Characterization of naked mole-rat hematopoiesis reveals unique stem and progenitor cell patterns and neotenic traits.

Applications

Unspecified application

Species

Unspecified reactive species

Stephan Emmrich,Alexandre Trapp,Frances Tolibzoda Zakusilo,Maggie E Straight,Albert K Ying,Alexander Tyshkovskiy,Marco Mariotti,Spencer Gray,Zhihui Zhang,Michael G Drage,Masaki Takasugi,Jan-Henning Klusmann,Vadim N Gladyshev,Andrei Seluanov,Vera Gorbunova

Journal of cell science 134: PubMed33558312

2021

Vimentin tunes cell migration on collagen by controlling β1 integrin activation and clustering.

Applications

Unspecified application

Species

Unspecified reactive species

Zofia Ostrowska-Podhorodecka,Isabel Ding,Wilson Lee,Jelena Tanic,Sevil Abbasi,Pamma D Arora,Richard S Liu,Alison E Patteson,Paul A Janmey,Christopher A McCulloch

Nature communications 12:56 PubMed33397922

2021

A RAC-GEF network critical for early intestinal tumourigenesis.

Applications

Unspecified application

Species

Unspecified reactive species

K A Pickering,K Gilroy,J W Cassidy,S K Fey,A K Najumudeen,L B Zeiger,D F Vincent,D M Gay,J Johansson,R P Fordham,B Miller,W Clark,A Hedley,E B Unal,C Kiel,E McGhee,L M Machesky,C Nixon,A E Johnsson,M Bain,D Strathdee,S R van Hoof,J P Medema,K I Anderson,S M Brachmann,V M Stucke,A Malliri,M Drysdale,M Turner,L Serrano,K Myant,A D Campbell,O J Sansom

Journal of ovarian research 13:90 PubMed32772928

2020

MiR-23a induced the activation of CDC42/PAK1 pathway and cell cycle arrest in human cov434 cells by targeting FGD4.

Applications

Unspecified application

Species

Unspecified reactive species

Ji Lin,Huijuan Huang,Liheng Lin,Weiwei Li,Jianfen Huang

Developmental dynamics : an official publication of the American Association of Anatomists 248:795-812 PubMed31219639

2019

Cdc42 activation by endothelin regulates neural crest cell migration in the cardiac outflow tract.

Applications

Unspecified application

Species

Unspecified reactive species

Katrina R Fritz,Yanping Zhang,L Bruno Ruest

Cellular and molecular life sciences : CMLS 76:2593-2614 PubMed30830239

2019

Rab6 regulates cell migration and invasion by recruiting Cdc42 and modulating its activity.

Applications

Unspecified application

Species

Unspecified reactive species

Katharina Vestre,Ingrid Kjos,Noemi Antonella Guadagno,Marita Borg Distefano,Felix Kohler,Federico Fenaroli,Oddmund Bakke,Cinzia Progida

Nature 563:514-521 PubMed30356216

2018

Mechanoresponsive stem cells acquire neural crest fate in jaw regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Ryan C Ransom,Ava C Carter,Ankit Salhotra,Tripp Leavitt,Owen Marecic,Matthew P Murphy,Michael L Lopez,Yuning Wei,Clement D Marshall,Ethan Z Shen,Ruth Ellen Jones,Amnon Sharir,Ophir D Klein,Charles K F Chan,Derrick C Wan,Howard Y Chang,Michael T Longaker
View all publications
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Product promise

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