Anti-CDC42 antibody [EPR15620] - Low endotoxin, Azide free

7 product images

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  Western blot - Anti-CDC42 antibody [EPR15620] - Low endotoxin, Azide free (ab221781)

  This data was developed using the same antibody clone in a different buffer formulation (Anti-CDC42 antibody [EPR15620] ab187643).
  

  Lanes 1-4: Merged signal (red and green). Green - Anti-CDC42 antibody [EPR15620] ab187643 observed at 20 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 37 kDa.

  Anti-CDC42 antibody [EPR15620] ab187643 Anti-CDC42 antibody [EPR15620] was shown to specifically react with CDC42 in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line Human CDC42 knockout HEK-293T cell line ab266522 (knockout cell lysate Human CDC42 knockout HEK-293T cell lysate ab256868) was used. Wild-type and CDC42 knockout samples were subjected to SDS-PAGE. Anti-CDC42 antibody [EPR15620] ab187643 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 5000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  All lanes: Western blot - Anti-CDC42 antibody [EPR15620] (Anti-CDC42 antibody [EPR15620] ab187643) at 1/5000 dilution

  Lane 1: Wild-type HEK-293T cell lysate at 20 µg

  Lane 2: CDC42 knockout HEK-293T cell lysate at 20 µg

  Lane 2: Western blot - Human CDC42 knockout HEK-293T cell line (Human CDC42 knockout HEK-293T cell line ab266522)

  Lane 3: MCF7 cell lysate at 20 µg

  Lane 4: HepG2 cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/20000 dilution

  Performed under reducing conditions.

  Predicted band size: 21 kDa

  Observed band size: 20 kDa

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  Immunocytochemistry/ Immunofluorescence - Anti-CDC42 antibody [EPR15620] - Low endotoxin, Azide free (ab221781)

  Immunocytochemistry/Immunofluorescence analysis of U937 (Human histiocytic lymphoma cell line) labelling CDC42 with purified Anti-CDC42 antibody [EPR15620] ab187643 at 1/500. Cells were fixed with 4% PFA and permeabilized with 0.1% triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat anti rabbit IgG (Alexa Fluor^® 488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CDC42 antibody [EPR15620] ab187643).

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC42 antibody [EPR15620] - Low endotoxin, Azide free (ab221781)

  Anti-CDC42 antibody [EPR15620] ab187643 staining CDC42 in Human lung carcinoma tissue sections by Immunohistochemistry (IHC-P- paraformaldehyde-fixed, paraffin-embedded sections). Antigen retrieval was by heat mediation using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).  Samples were incubated with primary antibody at a 1/500 dilution. A ready to use Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Hematoxylin was used as a counter stain. Cytoplasmic staining on human lung carcinoma.

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CDC42 antibody [EPR15620] ab187643).

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC42 antibody [EPR15620] - Low endotoxin, Azide free (ab221781)

  Anti-CDC42 antibody [EPR15620] ab187643 staining CDC42 in Mouse colon tissue sections by Immunohistochemistry (IHC-P- paraformaldehyde-fixed, paraffin-embedded sections).  Antigen retrieval was by heat mediation using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).  Samples were incubated with primary antibody at a 1/500 dilution. A ready to use Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Hematoxylin was used as a counter stain. Cytoplasmic staining on mouse colon.

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CDC42 antibody [EPR15620] ab187643).

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC42 antibody [EPR15620] - Low endotoxin, Azide free (ab221781)

  Anti-CDC42 antibody [EPR15620] ab187643 staining CDC42 in Rat colon tissue sections by Immunohistochemistry (IHC-P- paraformaldehyde-fixed, paraffin-embedded sections).  Antigen retrieval was by heat mediation using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).  Samples were incubated with primary antibody at a 1/500 dilution. A ready to use Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Hematoxylin was used as a counter stain. Cytoplasmic staining on rat colon.

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CDC42 antibody [EPR15620] ab187643).

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC42 antibody [EPR15620] - Low endotoxin, Azide free (ab221781)

  Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling CDC42 with Anti-CDC42 antibody [EPR15620] ab187643 at 1/250 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
  

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CDC42 antibody [EPR15620] ab187643).

  Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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  Flow Cytometry (Intracellular) - Anti-CDC42 antibody [EPR15620] - Low endotoxin, Azide free (ab221781)

  Intracellular Flow Cytometry analysis of HeLa cells (human cervix adenocarcinoma epithelial). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Primary antibody was used at a 1/120 dilution (red). A Goat anti rabbit IgG (Alexa Fluor^® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) was used as the isotype control (black). Cell without incubation with primary antibody and secondary antibody (Blue).
  

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CDC42 antibody [EPR15620] ab187643).