Mouse Monoclonal CDC42 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Recombinant fragment - Rat, Human, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Rat Cell division control protein 42 homolog.
Images
![Western blot - Anti-CDC42 antibody [M430] (AB202280), expandable thumbnail](https://content.abcam.com/products/images/cdc42-antibody-m430-ab202280--western-blot-img124433.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CDC42 antibody [M430] (AB202280)")
![Western blot - Anti-CDC42 antibody [M430] (AB202280), expandable thumbnail](https://content.abcam.com/products/images/cdc42-antibody-m430-ab202280--western-blot-img41440.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CDC42 antibody [M430] (AB202280)")
![Immunocytochemistry/ Immunofluorescence - Anti-CDC42 antibody [M430] (AB202280), expandable thumbnail](https://content.abcam.com/products/images/cdc42-antibody-m430-ab202280--immunocytochemistry-immunofluorescence-img112491.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-CDC42 antibody [M430] (AB202280)")
Publications
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- iScience 26:1063802023Lateral adherens junctions mediate a supracellular actomyosin cortex in drosophila trachea.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRojalin Pradhan,Shubham Kumar,Renjith MathewPubMed 37009223
Key facts
- Isotype
- IgM
- Host species
- Mouse
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Full Length Protein corresponding to Rat Cell division control protein 42 homolog. Database link Q8CFN2
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Expected |
| Mouse | Tested | Expected |
| Rat | Expected | Tested |
| Chicken | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Cynomolgus monkey | Predicted | Predicted |
| Dog | Predicted | Predicted |
| Drosophila melanogaster | Predicted | Predicted |
| Pig | Predicted | Predicted |
| Recombinant fragment - Rat | Tested | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 | Notes - |
Species Recombinant fragment - Rat | Dilution info 1/500 | Notes - |
Species Human | Dilution info 1/500 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Cow, Dog, Pig, Drosophila melanogaster, Cynomolgus monkey | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/200 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Cow, Dog, Pig, Drosophila melanogaster, Cynomolgus monkey | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Rat | Dilution info - | Notes - |
Associated Products
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Target data
Function
Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Regulates the bipolar attachment of spindle microtubules to kinetochores before chromosome congression in metaphase (PubMed:15642749). Regulates cell migration (PubMed:17038317, PubMed:22843693). In neurons, plays a role in the extension and maintenance of the formation of filopodia, thin and actin-rich surface projections (PubMed:14978216). Required for DOCK10-mediated spine formation in Purkinje cells and hippocampal neurons. In podocytes, facilitates filopodia and podosomes formation upon DOCK11-activation (PubMed:33523862). Upon activation by CaMKII, modulates dendritic spine structural plasticity by relaying CaMKII transient activation to synapse-specific, long-term signaling (By similarity). Also plays a role in phagocytosis through organization of the F-actin cytoskeleton associated with forming phagocytic cups (PubMed:26465210). Upon activation by PLEKHG4B, involved in actin cytoskeletal remodeling during epithelial cell-cell junction formation (PubMed:33310911).
Alternative names
Cell division control protein 42 homolog, G25K GTP-binding protein, CDC42
Recommended products
Mouse Monoclonal CDC42 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Recombinant fragment - Rat, Human, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Rat Cell division control protein 42 homolog.
Key facts
- Isotype
- IgM
- Host species
- Mouse
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Rat Cell division control protein 42 homolog. Database link Q8CFN2
- Clone number
- M430
- Purification technique
- Affinity purification Immunogen
- Specificity
ab202280 does not recognize a full length human RhoA GST fusion protein.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-CDC42 antibody [M430] (ab202280), expandable thumbnail](https://content.abcam.com/products/images/cdc42-antibody-m430-ab202280--western-blot-img124433.jpg "Western blot - Anti-CDC42 antibody [M430] (ab202280)")
Western blot - Anti-CDC42 antibody [M430] (ab202280)
CDC42 Western blot staining using mouse Anti-CDC42 antibody
Lane 1: Wild-type HAP1 cell lysate (20 μg)
Lane 2: CDC42 knockout HAP1 cell lysate (20 μg)
Lane 3: MCF7 cell lysate (20 μg)
Lane 4: HepG2 cell lysate (20 μg)
Lanes 1 - 4: Merged signal (red and green). Green - ab202280 observed at 20 kDa. Red - loading control, Anti-GAPDH antibody [EPR16891] - Loading Control ab181602, observed at 37 kDa.ab202280 was shown to recognize CDC42 in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when CDC42 knockout samples were examined. Wild-type and CDC42 knockout samples were subjected to SDS-PAGE. ab202280 and Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 (loading control to GAPDH) were diluted at 1/500 and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-CDC42 antibody [M430] (ab202280)
Predicted band size: 21 kDa
![Western blot - Anti-CDC42 antibody [M430] (ab202280), expandable thumbnail](https://content.abcam.com/products/images/cdc42-antibody-m430-ab202280--western-blot-img41440.jpg "Western blot - Anti-CDC42 antibody [M430] (ab202280)")
Western blot - Anti-CDC42 antibody [M430] (ab202280)
CDC42 Western blot staining using mouse Anti-CDC42 antibody
All lanes: Western blot - Anti-CDC42 antibody [M430] (ab202280) at 1/500 dilution
Lane 1: Jurkat cell lysate
Lane 2: mouse brain lysate
Lane 3: Recombiant full length rat Cdc42 protein
Predicted band size: 21 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-CDC42 antibody [M430] (ab202280), expandable thumbnail](https://content.abcam.com/products/images/cdc42-antibody-m430-ab202280--immunocytochemistry-immunofluorescence-img112491.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CDC42 antibody [M430] (ab202280)")
Immunocytochemistry/ Immunofluorescence - Anti-CDC42 antibody [M430] (ab202280)
Immunofluorescence analysis of paraformaldehyde-fixed rat A7r5 cells, labeling CDC42 using ab202280 at a 1/200 dilution, in the absence (left, black protein block) or presence (right, red image) of full length Cdc42 recombinant protein. Goat anti-mouse conjugated to DyLight® 594 was used as secondary antibody.
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