AB202280

Anti-CDC42 antibody [M430]

KO Validated
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(2 Publications)

Mouse Monoclonal CDC42 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Recombinant fragment - Rat, Human, Rat samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Rat Cell division control protein 42 homolog.

View Alternative Names

Cell division control protein 42 homolog, G25K GTP-binding protein, CDC42

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-CDC42 antibody [M430] (AB202280)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CDC42 antibody [M430] (AB202280)

Immunofluorescence analysis of paraformaldehyde-fixed rat A7r5 cells, labeling CDC42 using ab202280 at a 1/200 dilution, in the absence (left, black protein block) or presence (right, red image) of full length Cdc42 recombinant protein. Goat anti-mouse conjugated to DyLight® 594 was used as secondary antibody.

Lab

Western blot - Anti-CDC42 antibody [M430] (AB202280)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : CDC42 knockout HAP1 cell lysate (20 μg)
Lane 3 : MCF7 cell lysate (20 μg)
Lane 4 : HepG2 cell lysate (20 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab202280 observed at 20 kDa. Red - loading control, ab181602, observed at 37 kDa.

ab202280 was shown to recognize CDC42 in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when CDC42 knockout samples were examined. Wild-type and CDC42 knockout samples were subjected to SDS-PAGE. ab202280 and ab181602 (loading control to GAPDH) were diluted at 1/500 and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye^® 800CW) preadsorbed ab216772 and Goat Anti-Rabbit IgG H&L (IRDye^® 680RD) preadsorbed ab216777 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-CDC42 antibody [M430] (ab202280)

Predicted band size: 21 kDa

false

Supplier Data

Western blot - Anti-CDC42 antibody [M430] (AB202280)

All lanes:

Western blot - Anti-CDC42 antibody [M430] (ab202280) at 1/500 dilution

Lane 1:

Jurkat cell lysate

Lane 2:

mouse brain lysate

Lane 3:

Recombiant full length rat Cdc42 protein

Predicted band size: 21 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

M430

Isotype

IgM

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Rat Cell division control protein 42 homolog.

Q8CFN2

Specificity

ab202280 does not recognize a full length human RhoA GST fusion protein.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200", "ICCIF-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cynomolgus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Drosophila melanogaster": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Recombinant fragment - Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Regulates the bipolar attachment of spindle microtubules to kinetochores before chromosome congression in metaphase (PubMed : 15642749). Regulates cell migration (PubMed : 17038317, PubMed : 22843693). In neurons, plays a role in the extension and maintenance of the formation of filopodia, thin and actin-rich surface projections (PubMed : 14978216). Required for DOCK10-mediated spine formation in Purkinje cells and hippocampal neurons. In podocytes, facilitates filopodia and podosomes formation upon DOCK11-activation (PubMed : 33523862). Upon activation by CaMKII, modulates dendritic spine structural plasticity by relaying CaMKII transient activation to synapse-specific, long-term signaling (By similarity). Also plays a role in phagocytosis through organization of the F-actin cytoskeleton associated with forming phagocytic cups (PubMed : 26465210). Upon activation by PLEKHG4B, involved in actin cytoskeletal remodeling during epithelial cell-cell junction formation (PubMed : 33310911).

See full target information CDC42

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine (Cambridge, Mass.) 31:291 PubMed41013205

2025

YULINK deficiency promotes cell death under glucose restriction in HCC cells in association with GLUT1-mediated glycolysis.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Chia Wu,Tsai-Hsien Hung,Wei-Ting Thomas Wang,Ming-Wei Kuo,Yuh-Jin Liang,Yur-Ren Kuo,Ming-Feng Hou,Chung-Sheng Lai,Alice L Yu,John Yu

iScience 26:106380 PubMed37009223

2023

Lateral adherens junctions mediate a supracellular actomyosin cortex in drosophila trachea.

Applications

Unspecified application

Species

Unspecified reactive species

Rojalin Pradhan,Shubham Kumar,Renjith Mathew

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CDC42 antibody [M430]

Immunocytochemistry/ Immunofluorescence - Anti-CDC42 antibody [M430] (AB202280)

Western blot - Anti-CDC42 antibody [M430] (AB202280)

Western blot - Anti-CDC42 antibody [M430] (AB202280)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (2)

Product promise