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AB96659

Anti-CDC42 binding protein kinase alpha antibody

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(5 Publications)

Rabbit Polyclonal CDC42 binding protein kinase alpha antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CDC42BPA aa 350-600.

View Alternative Names

KIAA0451, CDC42BPA, Serine/threonine-protein kinase MRCK alpha, CDC42-binding protein kinase alpha, DMPK-like alpha, Myotonic dystrophy kinase-related CDC42-binding kinase alpha, MRCK alpha, Myotonic dystrophy protein kinase-like alpha

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC42 binding protein kinase alpha antibody (AB96659)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC42 binding protein kinase alpha antibody (AB96659)

ab96659 staining CDC42 binding protein kinase alpha in SW480 xenograft at 1/100 dilution by Immunohistochemistry (Formalin/PFA-Fixed Paraffin-embedded sections)

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human CDC42BPA aa 350-600. The exact immunogen used to generate this antibody is proprietary information.

Q5VT25

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>Perform heat-mediated antigen retrieval with 10mM Citrate buffer or Tris-EDTA buffer (pH 8.0)</p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Serine/threonine-protein kinase which is an important downstream effector of CDC42 and plays a role in the regulation of cytoskeleton reorganization and cell migration (PubMed : 15723050, PubMed : 9092543, PubMed : 9418861). Regulates actin cytoskeletal reorganization via phosphorylation of PPP1R12C and MYL9/MLC2 (PubMed : 21457715). In concert with MYO18A and LURAP1, is involved in modulating lamellar actomyosin retrograde flow that is crucial to cell protrusion and migration (PubMed : 18854160). Phosphorylates : PPP1R12A, LIMK1 and LIMK2 (PubMed : 11340065, PubMed : 11399775). May play a role in TFRC-mediated iron uptake (PubMed : 20188707). In concert with FAM89B/LRAP25 mediates the targeting of LIMK1 to the lamellipodium resulting in its activation and subsequent phosphorylation of CFL1 which is important for lamellipodial F-actin regulation (By similarity). Triggers the formation of an extrusion apical actin ring required for epithelial extrusion of apoptotic cells (PubMed : 29162624).
See full target information CDC42BPA
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Animal nutrition (Zhongguo xu mu shou yi xue hui) 10:319-328 PubMed35891685

2022

MRCKα is a novel regulator of prolactin-induced lactogenesis in bovine mammary epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Fang Wang,Jürgen van Baal,Lu Ma,Xuejun Gao,Jan Dijkstra,Dengpan Bu

Science signaling 13: PubMed32071169

2020

Functional proteomics interrogation of the kinome identifies MRCKA as a therapeutic target in high-grade serous ovarian carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Alison M Kurimchak,Carlos Herrera-Montávez,Jennifer Brown,Katherine J Johnson,Valerie Sodi,Nishi Srivastava,Vikas Kumar,Safoora Deihimi,Shane O'Brien,Suraj Peri,Gina M Mantia-Smaldone,Angela Jain,Ryan M Winters,Kathy Q Cai,Jonathan Chernoff,Denise C Connolly,James S Duncan

Schizophrenia research 201:270-277 PubMed29759351

2018

Dysbindin-1 contributes to prefrontal cortical dendritic arbor pathology in schizophrenia.

Applications

Unspecified application

Species

Unspecified reactive species

Glenn T Konopaske,Darrick T Balu,Kendall T Presti,Grace Chan,Francine M Benes,Joseph T Coyle

Cancer research 78:2096-2114 PubMed29382705

2018

Discovery of Potent and Selective MRCK Inhibitors with Therapeutic Effect on Skin Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Mathieu Unbekandt,Simone Belshaw,Justin Bower,Maeve Clarke,Jacqueline Cordes,Diane Crighton,Daniel R Croft,Martin J Drysdale,Mathew J Garnett,Kathryn Gill,Christopher Gray,David A Greenhalgh,James A M Hall,Jennifer Konczal,Sergio Lilla,Duncan McArthur,Patricia McConnell,Laura McDonald,Lynn McGarry,Heather McKinnon,Carol McMenemy,Mokdad Mezna,Nicolas A Morrice,June Munro,Gregory Naylor,Nicola Rath,Alexander W Schüttelkopf,Mairi Sime,Michael F Olson

Oncology letters 10:2359-2365 PubMed26622852

2015

Afzelin exhibits anti-cancer activity against androgen-sensitive LNCaP and androgen-independent PC-3 prostate cancer cells through the inhibition of LIM domain kinase 1.

Applications

WB

Species

Human

Kai-Chang Zhu,Jian-Mei Sun,Jian-Guo Shen,Ji-Zhong Jin,Feng Liu,Xiao-Lin Xu,Lin Chen,Lin-Tao Liu,Jia-Ju Lv
View all publications
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Product promise

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