Rabbit Polyclonal CDC42 binding protein kinase alpha antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CDC42BPA aa 350-600.
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Publications
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- Animal nutrition (Zhongguo xu mu shou yi xue hui) 10:319-3282022MRCKα is a novel regulator of prolactin-induced lactogenesis in bovine mammary epithelial cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFang Wang,Jürgen van Baal,Lu Ma,Xuejun Gao,Jan Dijkstra,Dengpan BuPubMed 35891685
- Science signaling 13:2020Functional proteomics interrogation of the kinome identifies MRCKA as a therapeutic target in high-grade serous ovarian carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlison M Kurimchak,Carlos Herrera-Montávez,Jennifer Brown,Katherine J Johnson,Valerie Sodi,Nishi Srivastava,Vikas Kumar,Safoora Deihimi,Shane O'Brien,Suraj Peri,Gina M Mantia-Smaldone,Angela Jain,Ryan M Winters,Kathy Q Cai,Jonathan Chernoff,Denise C Connolly,James S DuncanPubMed 32071169
- Schizophrenia research 201:270-2772018Dysbindin-1 contributes to prefrontal cortical dendritic arbor pathology in schizophrenia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGlenn T Konopaske et. al.PubMed 29759351
- Cancer research 78:2096-21142018Discovery of Potent and Selective MRCK Inhibitors with Therapeutic Effect on Skin Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMathieu Unbekandt,Simone Belshaw,Justin Bower,Maeve Clarke,Jacqueline Cordes,Diane Crighton,Daniel R Croft,Martin J Drysdale,Mathew J Garnett,Kathryn Gill,Christopher Gray,David A Greenhalgh,James A M Hall,Jennifer Konczal,Sergio Lilla,Duncan McArthur,Patricia McConnell,Laura McDonald,Lynn McGarry,Heather McKinnon,Carol McMenemy,Mokdad Mezna,Nicolas A Morrice,June Munro,Gregory Naylor,Nicola Rath,Alexander W Schüttelkopf,Mairi Sime,Michael F OlsonPubMed 29382705
- Oncology letters 10:2359-23652015Afzelin exhibits anti-cancer activity against androgen-sensitive LNCaP and androgen-independent PC-3 prostate cancer cells through the inhibition of LIM domain kinase 1.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 59.99% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human CDC42BPA aa 350-600. The exact immunogen used to generate this antibody is proprietary information. Database link Q5VT25
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with 10mM Citrate buffer or Tris-EDTA buffer (pH 8.0) |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Serine/threonine-protein kinase which is an important downstream effector of CDC42 and plays a role in the regulation of cytoskeleton reorganization and cell migration (PubMed:15723050, PubMed:9092543, PubMed:9418861). Regulates actin cytoskeletal reorganization via phosphorylation of PPP1R12C and MYL9/MLC2 (PubMed:21457715). In concert with MYO18A and LURAP1, is involved in modulating lamellar actomyosin retrograde flow that is crucial to cell protrusion and migration (PubMed:18854160). Phosphorylates: PPP1R12A, LIMK1 and LIMK2 (PubMed:11340065, PubMed:11399775). May play a role in TFRC-mediated iron uptake (PubMed:20188707). In concert with FAM89B/LRAP25 mediates the targeting of LIMK1 to the lamellipodium resulting in its activation and subsequent phosphorylation of CFL1 which is important for lamellipodial F-actin regulation (By similarity). Triggers the formation of an extrusion apical actin ring required for epithelial extrusion of apoptotic cells (PubMed:29162624).
Alternative names
KIAA0451, CDC42BPA, Serine/threonine-protein kinase MRCK alpha, CDC42-binding protein kinase alpha, DMPK-like alpha, Myotonic dystrophy kinase-related CDC42-binding kinase alpha, MRCK alpha, Myotonic dystrophy protein kinase-like alpha
Recommended products
Rabbit Polyclonal CDC42 binding protein kinase alpha antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CDC42BPA aa 350-600.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 59.99% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human CDC42BPA aa 350-600. The exact immunogen used to generate this antibody is proprietary information. Database link Q5VT25
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Western blot - Anti-CDC42 binding protein kinase alpha antibody (ab96659)
7.5% SDS PAGE
All lanes: Western blot - Anti-CDC42 binding protein kinase alpha antibody (ab96659) at 1/1500 dilution
All lanes: H1299 whole cell lysate at 30 µg
Predicted band size: 197 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDC42 binding protein kinase alpha antibody (ab96659)
ab96659 staining CDC42 binding protein kinase alpha in SW480 xenograft at 1/100 dilution by Immunohistochemistry (Formalin/PFA-Fixed Paraffin-embedded sections)
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